A simple and cheaper in house varicella zoster virus antibody indirect ELISA

Detalhes bibliográficos
Autor(a) principal: Ono, Erika [UNIFESP]
Data de Publicação: 2004
Outros Autores: Lafer, Manuel Mindlin [UNIFESP], Weckx, Lily Yin [UNIFESP], Granato, Celso Francisco Hernandes [UNIFESP], De Moraes-Pinto, Maria Isabel [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0036-46652004000300008
http://repositorio.unifesp.br/handle/11600/2133
Resumo: We have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre. Also, the addition of an extra step with 8M urea allowed the assessment of VZV IgG avidity without excessive costs. The cost per sample to test VZV IgG was 2.7 times cheaper with our ELISA, allowing the testing of many samples without the burden of production of VZV antigen in the laboratory.
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spelling A simple and cheaper in house varicella zoster virus antibody indirect ELISADesenvolvimento de ELISA indireto simples e de baixo custo para detecção de anticorpos anti-varicela zosterVaricellaELISAAntibody avidityWe have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre. Also, the addition of an extra step with 8M urea allowed the assessment of VZV IgG avidity without excessive costs. The cost per sample to test VZV IgG was 2.7 times cheaper with our ELISA, allowing the testing of many samples without the burden of production of VZV antigen in the laboratory.Desenvolvemos um ensaio imunoenzimático (ELISA) indireto simples e econômico para detecção de anticorpos contra o vírus da varicela zoster (VVZ) que utiliza a vacina contendo o vírus vivo atenuado como antígeno para recobrir a placa. Este ELISA mostrou uma concordância de 94% quando comparado com um kit de ELISA comercial para anticorpos contra varicela. Além disso, nosso ELISA mostrou ser mais confiável que o kit quando amostras comprovadamente negativas foram testadas. O uso de um soro padrão de referência, calibrado em unidades internacionais por mililitro, possibilitou também que os resultados pudessem ser comparados com outros estudos. O acréscimo de uma etapa extra com solução de uréia 8M permitiu avaliação de avidez de IgG para VVZ sem custos excessivos. O custo por amostra para testar IgG contra VVZ com nosso ELISA foi 2,7 vezes mais barato quando comparado com o kit comercial.Federal University of São Paulo Division of Pediatric Infectious DiseasesFederal University of São Paulo Division of Adult Infectious DiseasesFleury Laboratory Immunology DepartmentUNIFESP, Division of Pediatric Infectious DiseasesUNIFESP, Division of Adult Infectious DiseasesSciELOInstituto de Medicina TropicalUniversidade Federal de São Paulo (UNIFESP)Fleury Laboratory Immunology DepartmentOno, Erika [UNIFESP]Lafer, Manuel Mindlin [UNIFESP]Weckx, Lily Yin [UNIFESP]Granato, Celso Francisco Hernandes [UNIFESP]De Moraes-Pinto, Maria Isabel [UNIFESP]2015-06-14T13:31:10Z2015-06-14T13:31:10Z2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion165-168application/pdfhttp://dx.doi.org/10.1590/S0036-46652004000300008Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical, v. 46, n. 3, p. 165-168, 2004.10.1590/S0036-46652004000300008S0036-46652004000300008.pdf0036-4665S0036-46652004000300008http://repositorio.unifesp.br/handle/11600/2133engRevista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T14:33:08Zoai:repositorio.unifesp.br/:11600/2133Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T14:33:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A simple and cheaper in house varicella zoster virus antibody indirect ELISA
Desenvolvimento de ELISA indireto simples e de baixo custo para detecção de anticorpos anti-varicela zoster
title A simple and cheaper in house varicella zoster virus antibody indirect ELISA
spellingShingle A simple and cheaper in house varicella zoster virus antibody indirect ELISA
Ono, Erika [UNIFESP]
Varicella
ELISA
Antibody avidity
title_short A simple and cheaper in house varicella zoster virus antibody indirect ELISA
title_full A simple and cheaper in house varicella zoster virus antibody indirect ELISA
title_fullStr A simple and cheaper in house varicella zoster virus antibody indirect ELISA
title_full_unstemmed A simple and cheaper in house varicella zoster virus antibody indirect ELISA
title_sort A simple and cheaper in house varicella zoster virus antibody indirect ELISA
author Ono, Erika [UNIFESP]
author_facet Ono, Erika [UNIFESP]
Lafer, Manuel Mindlin [UNIFESP]
Weckx, Lily Yin [UNIFESP]
Granato, Celso Francisco Hernandes [UNIFESP]
De Moraes-Pinto, Maria Isabel [UNIFESP]
author_role author
author2 Lafer, Manuel Mindlin [UNIFESP]
Weckx, Lily Yin [UNIFESP]
Granato, Celso Francisco Hernandes [UNIFESP]
De Moraes-Pinto, Maria Isabel [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fleury Laboratory Immunology Department
dc.contributor.author.fl_str_mv Ono, Erika [UNIFESP]
Lafer, Manuel Mindlin [UNIFESP]
Weckx, Lily Yin [UNIFESP]
Granato, Celso Francisco Hernandes [UNIFESP]
De Moraes-Pinto, Maria Isabel [UNIFESP]
dc.subject.por.fl_str_mv Varicella
ELISA
Antibody avidity
topic Varicella
ELISA
Antibody avidity
description We have developed a cheaper an simple in house indirect ELISA that uses the live attenuated VZV vaccine as a coating antigen. The alternative ELISA had an agreement of 94% when compared with a commercial VZV ELISA kit. Moreover, our ELISA proved to be more reliable than the kit when assessing true negative samples. By adding a standard serum, we were able to produce results in international units per millilitre. Also, the addition of an extra step with 8M urea allowed the assessment of VZV IgG avidity without excessive costs. The cost per sample to test VZV IgG was 2.7 times cheaper with our ELISA, allowing the testing of many samples without the burden of production of VZV antigen in the laboratory.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
2015-06-14T13:31:10Z
2015-06-14T13:31:10Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0036-46652004000300008
Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical, v. 46, n. 3, p. 165-168, 2004.
10.1590/S0036-46652004000300008
S0036-46652004000300008.pdf
0036-4665
S0036-46652004000300008
http://repositorio.unifesp.br/handle/11600/2133
url http://dx.doi.org/10.1590/S0036-46652004000300008
http://repositorio.unifesp.br/handle/11600/2133
identifier_str_mv Revista do Instituto de Medicina Tropical de São Paulo. Instituto de Medicina Tropical, v. 46, n. 3, p. 165-168, 2004.
10.1590/S0036-46652004000300008
S0036-46652004000300008.pdf
0036-4665
S0036-46652004000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 165-168
application/pdf
dc.publisher.none.fl_str_mv Instituto de Medicina Tropical
publisher.none.fl_str_mv Instituto de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268371475103744

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