Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28606 http://dx.doi.org/10.1099/jmm.0.46218-0 |
Resumo: | Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. in both patients, using genetic analysis procedures such as PCR-restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA MYCOBACTERIA) and/or sequencing analysis of the 16S-23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species. |
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Suffys, P.Silva Rocha, A. daBrandao, A.Vanderborght, B.Mijs, W.Jannes, G.Mello, FCQPedro, HSPFonseca, LDSOliveira, R. S. deLeao, S. C.Saad, MHFFiocruz MSUniversidade Federal do Rio de Janeiro (UFRJ)Innogenet NVAdolfo Lutz InstUniversidade Federal de São Paulo (UNIFESP)2016-01-24T12:38:14Z2016-01-24T12:38:14Z2006-01-01Journal of Medical Microbiology. Reading: Soc General Microbiology, v. 55, n. 1, p. 127-131, 2006.0022-2615http://repositorio.unifesp.br/handle/11600/28606http://dx.doi.org/10.1099/jmm.0.46218-010.1099/jmm.0.46218-0WOS:000234357200019Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. in both patients, using genetic analysis procedures such as PCR-restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA MYCOBACTERIA) and/or sequencing analysis of the 16S-23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species.Fiocruz MS, Inst Oswaldo Cruz, Dept Mycobacterioses, Rio de Janeiro, BrazilFiocruz MS, Inst Oswaldo Cruz, Dept Trop Med, Rio de Janeiro, BrazilUFRJ, Univ Hosp Clementino Fraga Filho, Rio de Janeiro, BrazilUFRJ, Inst Microbiol, Rio de Janeiro, BrazilInnogenet NV, B-9052 Ghent, BelgiumAdolfo Lutz Inst, São Paulo, BrazilEscola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilEscola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of Science127-131engSoc General MicrobiologyJournal of Medical MicrobiologyDetection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/286062023-01-27 22:45:17.056metadata only accessoai:repositorio.unifesp.br:11600/28606Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-28T01:45:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
title |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
spellingShingle |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods Suffys, P. |
title_short |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
title_full |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
title_fullStr |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
title_full_unstemmed |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
title_sort |
Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods |
author |
Suffys, P. |
author_facet |
Suffys, P. Silva Rocha, A. da Brandao, A. Vanderborght, B. Mijs, W. Jannes, G. Mello, FCQ Pedro, HSP Fonseca, LDS Oliveira, R. S. de Leao, S. C. Saad, MHF |
author_role |
author |
author2 |
Silva Rocha, A. da Brandao, A. Vanderborght, B. Mijs, W. Jannes, G. Mello, FCQ Pedro, HSP Fonseca, LDS Oliveira, R. S. de Leao, S. C. Saad, MHF |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Fiocruz MS Universidade Federal do Rio de Janeiro (UFRJ) Innogenet NV Adolfo Lutz Inst Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Suffys, P. Silva Rocha, A. da Brandao, A. Vanderborght, B. Mijs, W. Jannes, G. Mello, FCQ Pedro, HSP Fonseca, LDS Oliveira, R. S. de Leao, S. C. Saad, MHF |
description |
Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. in both patients, using genetic analysis procedures such as PCR-restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA MYCOBACTERIA) and/or sequencing analysis of the 16S-23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:38:14Z |
dc.date.available.fl_str_mv |
2016-01-24T12:38:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Medical Microbiology. Reading: Soc General Microbiology, v. 55, n. 1, p. 127-131, 2006. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28606 http://dx.doi.org/10.1099/jmm.0.46218-0 |
dc.identifier.issn.none.fl_str_mv |
0022-2615 |
dc.identifier.doi.none.fl_str_mv |
10.1099/jmm.0.46218-0 |
dc.identifier.wos.none.fl_str_mv |
WOS:000234357200019 |
identifier_str_mv |
Journal of Medical Microbiology. Reading: Soc General Microbiology, v. 55, n. 1, p. 127-131, 2006. 0022-2615 10.1099/jmm.0.46218-0 WOS:000234357200019 |
url |
http://repositorio.unifesp.br/handle/11600/28606 http://dx.doi.org/10.1099/jmm.0.46218-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Medical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
127-131 |
dc.publisher.none.fl_str_mv |
Soc General Microbiology |
publisher.none.fl_str_mv |
Soc General Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764276313620480 |