Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://www.irog.net/download/?magazine=91 http://repositorio.unifesp.br/handle/11600/43599 |
Resumo: | Purpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection. |
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Repositório Institucional da UNIFESP |
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Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3topoisomerase II alphacaspase-3HPVneoplasiaPurpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.Univ Fed Sao Paulo, UNIFESP EPM, Dept Gynecol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Pathol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Gynecol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Pathol, Sao Paulo, BrazilWeb of ScienceI R O G Canada, IncUniversidade Federal de São Paulo (UNIFESP)Coelho, Raquel Autran [UNIFESP]Focchi, Gustavo Rubino de Azevedo [UNIFESP]Nogueira-de-Souza, Naiara Corrêa [UNIFESP]Sartori, Marair Gracio Ferreira [UNIFESP]Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]Ribalta, Julisa Chamorro Lascasas [UNIFESP]2018-06-15T17:22:25Z2018-06-15T17:22:25Z2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion499-501http://www.irog.net/download/?magazine=91European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008.0392-2936http://repositorio.unifesp.br/handle/11600/43599WOS:000259328800017engEuropean Journal Of Gynaecological Oncologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:52:08Zoai:repositorio.unifesp.br/:11600/43599Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:52:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
title |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
spellingShingle |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 Coelho, Raquel Autran [UNIFESP] topoisomerase II alpha caspase-3 HPV neoplasia |
title_short |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
title_full |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
title_fullStr |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
title_full_unstemmed |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
title_sort |
Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3 |
author |
Coelho, Raquel Autran [UNIFESP] |
author_facet |
Coelho, Raquel Autran [UNIFESP] Focchi, Gustavo Rubino de Azevedo [UNIFESP] Nogueira-de-Souza, Naiara Corrêa [UNIFESP] Sartori, Marair Gracio Ferreira [UNIFESP] Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP] Ribalta, Julisa Chamorro Lascasas [UNIFESP] |
author_role |
author |
author2 |
Focchi, Gustavo Rubino de Azevedo [UNIFESP] Nogueira-de-Souza, Naiara Corrêa [UNIFESP] Sartori, Marair Gracio Ferreira [UNIFESP] Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP] Ribalta, Julisa Chamorro Lascasas [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Coelho, Raquel Autran [UNIFESP] Focchi, Gustavo Rubino de Azevedo [UNIFESP] Nogueira-de-Souza, Naiara Corrêa [UNIFESP] Sartori, Marair Gracio Ferreira [UNIFESP] Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP] Ribalta, Julisa Chamorro Lascasas [UNIFESP] |
dc.subject.por.fl_str_mv |
topoisomerase II alpha caspase-3 HPV neoplasia |
topic |
topoisomerase II alpha caspase-3 HPV neoplasia |
description |
Purpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2018-06-15T17:22:25Z 2018-06-15T17:22:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.irog.net/download/?magazine=91 European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008. 0392-2936 http://repositorio.unifesp.br/handle/11600/43599 WOS:000259328800017 |
url |
http://www.irog.net/download/?magazine=91 http://repositorio.unifesp.br/handle/11600/43599 |
identifier_str_mv |
European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008. 0392-2936 WOS:000259328800017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal Of Gynaecological Oncology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
499-501 |
dc.publisher.none.fl_str_mv |
I R O G Canada, Inc |
publisher.none.fl_str_mv |
I R O G Canada, Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268298516234240 |