Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3

Detalhes bibliográficos
Autor(a) principal: Coelho, Raquel Autran [UNIFESP]
Data de Publicação: 2008
Outros Autores: Focchi, Gustavo Rubino de Azevedo [UNIFESP], Nogueira-de-Souza, Naiara Corrêa [UNIFESP], Sartori, Marair Gracio Ferreira [UNIFESP], Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP], Ribalta, Julisa Chamorro Lascasas [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://www.irog.net/download/?magazine=91
http://repositorio.unifesp.br/handle/11600/43599
Resumo: Purpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.
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spelling Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3topoisomerase II alphacaspase-3HPVneoplasiaPurpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.Univ Fed Sao Paulo, UNIFESP EPM, Dept Gynecol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Pathol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Gynecol, Sao Paulo, BrazilUniv Fed Sao Paulo, UNIFESP EPM, Dept Pathol, Sao Paulo, BrazilWeb of ScienceI R O G Canada, IncUniversidade Federal de São Paulo (UNIFESP)Coelho, Raquel Autran [UNIFESP]Focchi, Gustavo Rubino de Azevedo [UNIFESP]Nogueira-de-Souza, Naiara Corrêa [UNIFESP]Sartori, Marair Gracio Ferreira [UNIFESP]Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]Ribalta, Julisa Chamorro Lascasas [UNIFESP]2018-06-15T17:22:25Z2018-06-15T17:22:25Z2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion499-501http://www.irog.net/download/?magazine=91European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008.0392-2936http://repositorio.unifesp.br/handle/11600/43599WOS:000259328800017engEuropean Journal Of Gynaecological Oncologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:52:08Zoai:repositorio.unifesp.br/:11600/43599Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:52:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
title Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
spellingShingle Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
Coelho, Raquel Autran [UNIFESP]
topoisomerase II alpha
caspase-3
HPV
neoplasia
title_short Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
title_full Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
title_fullStr Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
title_full_unstemmed Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
title_sort Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3
author Coelho, Raquel Autran [UNIFESP]
author_facet Coelho, Raquel Autran [UNIFESP]
Focchi, Gustavo Rubino de Azevedo [UNIFESP]
Nogueira-de-Souza, Naiara Corrêa [UNIFESP]
Sartori, Marair Gracio Ferreira [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Ribalta, Julisa Chamorro Lascasas [UNIFESP]
author_role author
author2 Focchi, Gustavo Rubino de Azevedo [UNIFESP]
Nogueira-de-Souza, Naiara Corrêa [UNIFESP]
Sartori, Marair Gracio Ferreira [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Ribalta, Julisa Chamorro Lascasas [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Coelho, Raquel Autran [UNIFESP]
Focchi, Gustavo Rubino de Azevedo [UNIFESP]
Nogueira-de-Souza, Naiara Corrêa [UNIFESP]
Sartori, Marair Gracio Ferreira [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Ribalta, Julisa Chamorro Lascasas [UNIFESP]
dc.subject.por.fl_str_mv topoisomerase II alpha
caspase-3
HPV
neoplasia
topic topoisomerase II alpha
caspase-3
HPV
neoplasia
description Purpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2018-06-15T17:22:25Z
2018-06-15T17:22:25Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.irog.net/download/?magazine=91
European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008.
0392-2936
http://repositorio.unifesp.br/handle/11600/43599
WOS:000259328800017
url http://www.irog.net/download/?magazine=91
http://repositorio.unifesp.br/handle/11600/43599
identifier_str_mv European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008.
0392-2936
WOS:000259328800017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal Of Gynaecological Oncology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 499-501
dc.publisher.none.fl_str_mv I R O G Canada, Inc
publisher.none.fl_str_mv I R O G Canada, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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