Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/0074-02760160196 https://repositorio.unifesp.br/handle/11600/55167 |
Resumo: | BACKGROUND In high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally. OBJECTIVE To evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories. METHODS A total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, Sao Paulo and Canoas). FINDINGS Seventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/ culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95% |
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Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Braziltuberculosisreverse-hybridisation assayIS6110molecular diagnosisDetect-TBBACKGROUND In high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally. OBJECTIVE To evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories. METHODS A total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, Sao Paulo and Canoas). FINDINGS Seventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/ culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95%73.7-91.6) and 93.1% (CI 95%89.195.8), respectively. When compared to bacteriological and clinical diagnostic criteria, the sensitivity and specificity of Detect-TB assay was 74.3% (CI 95%63.3-82.9) and 92.9% (CI 95%88.7-95.6), respectively. Among the three sites - Caxias do Sul, Sao Paulo and Canoas - the sensitivity and specificity were respectively 94.7% and 97.8%71.4% and 93.9%, 82.1% and 88.9%. MAIN CONCLUSIONS These findings suggest that the Detect-TB assay could be applied routinely in reference laboratories across different regions in Brazil.Fundacao Estadual Prod & Pesquisa Saude, Ctr Desenvolvimento Cient & Tecnol, Porto Alegre, RS, BrazilUniv Caxias Do Sul, Lab Pesquisa HIV AIDS, Caxias Do Sul, RS, BrazilUniv Caxias Do Sul, Lab Microbiol Clin, Caxias Do Sul, RS, BrazilUniv Fed Sao Paulo, Lab Especial Microbiol Clin, Sao Paulo, SP, BrazilUniv Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Fac Med, Ctr Pesquisa TB,Lab Genet & Biol Mol, Rio De Janeiro, RJ, BrazilLabtest, Belo Horizonte, MG, BrazilUniv Luterana Brasil, Canoas, RS, BrazilRede Brasileira Pesquisa TB, Rio De Janeiro, RJ, BrazilUniv Fed Sao Paulo, Lab Especial Microbiol Clin, Sao Paulo, SP, BrazilWeb of ScienceCNPq/INCTFINEPLabtestFEPPSCNPq/INCT: 573548/2008-0Fundaco Oswaldo Cruz2020-07-17T14:03:06Z2020-07-17T14:03:06Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion94-99application/pdfhttp://dx.doi.org/10.1590/0074-02760160196Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj, v. 112, n. 2, p. 94-99, 2017.10.1590/0074-02760160196WOS000394155800002.pdf0074-0276https://repositorio.unifesp.br/handle/11600/55167WOS:000394155800002engMemorias Do Instituto Oswaldo CruzRio De Janeiro, Rjinfo:eu-repo/semantics/openAccessVerza, MirelaSchmid, Karen BarrosBarcellos, Regina BonesLinck, NataliBello, Graziele LimaScapin, DanielSperhacke, Rosa DeaNunes Silva, Marcia SusanaWollheim, ClaudiaCelle Rivero, Martha Gabriela [UNIFESP]Kritski, Afranio LineuRezende, Leonides, Jr.Oliveira, Martha MariaDalla Costa, Elis ReginaRosa Rossetti, Maria Luciareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-09T14:46:52Zoai:repositorio.unifesp.br/:11600/55167Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-09T14:46:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| title |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| spellingShingle |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil Verza, Mirela tuberculosis reverse-hybridisation assay IS6110 molecular diagnosis Detect-TB |
| title_short |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| title_full |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| title_fullStr |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| title_full_unstemmed |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| title_sort |
Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil |
| author |
Verza, Mirela |
| author_facet |
Verza, Mirela Schmid, Karen Barros Barcellos, Regina Bones Linck, Natali Bello, Graziele Lima Scapin, Daniel Sperhacke, Rosa Dea Nunes Silva, Marcia Susana Wollheim, Claudia Celle Rivero, Martha Gabriela [UNIFESP] Kritski, Afranio Lineu Rezende, Leonides, Jr. Oliveira, Martha Maria Dalla Costa, Elis Regina Rosa Rossetti, Maria Lucia |
| author_role |
author |
| author2 |
Schmid, Karen Barros Barcellos, Regina Bones Linck, Natali Bello, Graziele Lima Scapin, Daniel Sperhacke, Rosa Dea Nunes Silva, Marcia Susana Wollheim, Claudia Celle Rivero, Martha Gabriela [UNIFESP] Kritski, Afranio Lineu Rezende, Leonides, Jr. Oliveira, Martha Maria Dalla Costa, Elis Regina Rosa Rossetti, Maria Lucia |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Verza, Mirela Schmid, Karen Barros Barcellos, Regina Bones Linck, Natali Bello, Graziele Lima Scapin, Daniel Sperhacke, Rosa Dea Nunes Silva, Marcia Susana Wollheim, Claudia Celle Rivero, Martha Gabriela [UNIFESP] Kritski, Afranio Lineu Rezende, Leonides, Jr. Oliveira, Martha Maria Dalla Costa, Elis Regina Rosa Rossetti, Maria Lucia |
| dc.subject.por.fl_str_mv |
tuberculosis reverse-hybridisation assay IS6110 molecular diagnosis Detect-TB |
| topic |
tuberculosis reverse-hybridisation assay IS6110 molecular diagnosis Detect-TB |
| description |
BACKGROUND In high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally. OBJECTIVE To evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories. METHODS A total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, Sao Paulo and Canoas). FINDINGS Seventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/ culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95% |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2020-07-17T14:03:06Z 2020-07-17T14:03:06Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760160196 Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj, v. 112, n. 2, p. 94-99, 2017. 10.1590/0074-02760160196 WOS000394155800002.pdf 0074-0276 https://repositorio.unifesp.br/handle/11600/55167 WOS:000394155800002 |
| url |
http://dx.doi.org/10.1590/0074-02760160196 https://repositorio.unifesp.br/handle/11600/55167 |
| identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj, v. 112, n. 2, p. 94-99, 2017. 10.1590/0074-02760160196 WOS000394155800002.pdf 0074-0276 WOS:000394155800002 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Memorias Do Instituto Oswaldo Cruz |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
94-99 application/pdf |
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Rio De Janeiro, Rj |
| dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
| publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268403389562880 |