Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.4103/1673-5374.224378 https://repositorio.unifesp.br/handle/11600/53863 |
Resumo: | Studies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MG |
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Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regenerationnerve regenerationmesenchymal stem cellsadipose-derived mesenchmal stem cellssciatic nerveMatrigelsciatic functional indexneural regenerationStudies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MGsciatic nerve injury + intravenous transplantation of MG vehicle), ADSCs (sciatic nerve injury + intravenous MG containing ADSCs), and BMSCs (sciatic nerve injury + intravenous MG containing BMSCs) groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury, increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios (axonal diameter/fiber diameter ratios) in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment.Univ Fed Sao Paulo, Dept Orthoped & Traumatol, Div Hand & Upper Limb Surg, Sao Paulo, BrazilUniv Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, BrazilUniv Fed Sao Paulo, Div Neurosci, Dept Neurol & Neurosurg, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Div Hand & Upper Limb Surg, Sao Paulo, BrazilUniv Fed Sao Paulo, Div Neurosci, Dept Neurol & Neurosurg, Sao Paulo, BrazilWeb of ScienceFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)CAPESMedknow Publications & Media Pvt Ltd2020-07-02T18:52:04Z2020-07-02T18:52:04Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion100-104application/pdfhttp://dx.doi.org/10.4103/1673-5374.224378Neural Regeneration Research. Mumbai, v. 13, n. 1, p. 100-104, 2018.10.4103/1673-5374.224378WOS000425047900016.pdf1673-5374https://repositorio.unifesp.br/handle/11600/53863WOS:000425047900016engNeural Regeneration ResearchMumbaiinfo:eu-repo/semantics/openAccessFernandes, Marcela [UNIFESP]Valente, Sandra Gomes [UNIFESP]Sabongi, Rodrigo Guerra [UNIFESP]Gomes dos Santos, Joao Baptista [UNIFESP]Leite, Vilnei Mattioli [UNIFESP]Ulrich, HenningNery, Arthur Andradeda Silva Fernandes, Maria Jose [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-11T04:04:38Zoai:repositorio.unifesp.br/:11600/53863Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-11T04:04:38Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| title |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| spellingShingle |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration Fernandes, Marcela [UNIFESP] nerve regeneration mesenchymal stem cells adipose-derived mesenchmal stem cells sciatic nerve Matrigel sciatic functional index neural regeneration |
| title_short |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| title_full |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| title_fullStr |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| title_full_unstemmed |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| title_sort |
Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration |
| author |
Fernandes, Marcela [UNIFESP] |
| author_facet |
Fernandes, Marcela [UNIFESP] Valente, Sandra Gomes [UNIFESP] Sabongi, Rodrigo Guerra [UNIFESP] Gomes dos Santos, Joao Baptista [UNIFESP] Leite, Vilnei Mattioli [UNIFESP] Ulrich, Henning Nery, Arthur Andrade da Silva Fernandes, Maria Jose [UNIFESP] |
| author_role |
author |
| author2 |
Valente, Sandra Gomes [UNIFESP] Sabongi, Rodrigo Guerra [UNIFESP] Gomes dos Santos, Joao Baptista [UNIFESP] Leite, Vilnei Mattioli [UNIFESP] Ulrich, Henning Nery, Arthur Andrade da Silva Fernandes, Maria Jose [UNIFESP] |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fernandes, Marcela [UNIFESP] Valente, Sandra Gomes [UNIFESP] Sabongi, Rodrigo Guerra [UNIFESP] Gomes dos Santos, Joao Baptista [UNIFESP] Leite, Vilnei Mattioli [UNIFESP] Ulrich, Henning Nery, Arthur Andrade da Silva Fernandes, Maria Jose [UNIFESP] |
| dc.subject.por.fl_str_mv |
nerve regeneration mesenchymal stem cells adipose-derived mesenchmal stem cells sciatic nerve Matrigel sciatic functional index neural regeneration |
| topic |
nerve regeneration mesenchymal stem cells adipose-derived mesenchmal stem cells sciatic nerve Matrigel sciatic functional index neural regeneration |
| description |
Studies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MG |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2020-07-02T18:52:04Z 2020-07-02T18:52:04Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4103/1673-5374.224378 Neural Regeneration Research. Mumbai, v. 13, n. 1, p. 100-104, 2018. 10.4103/1673-5374.224378 WOS000425047900016.pdf 1673-5374 https://repositorio.unifesp.br/handle/11600/53863 WOS:000425047900016 |
| url |
http://dx.doi.org/10.4103/1673-5374.224378 https://repositorio.unifesp.br/handle/11600/53863 |
| identifier_str_mv |
Neural Regeneration Research. Mumbai, v. 13, n. 1, p. 100-104, 2018. 10.4103/1673-5374.224378 WOS000425047900016.pdf 1673-5374 WOS:000425047900016 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Neural Regeneration Research |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
100-104 application/pdf |
| dc.coverage.none.fl_str_mv |
Mumbai |
| dc.publisher.none.fl_str_mv |
Medknow Publications & Media Pvt Ltd |
| publisher.none.fl_str_mv |
Medknow Publications & Media Pvt Ltd |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268323513237504 |