Urinary beta-trace protein gene expression analysis in type 2 diabetes mellitus patients

• Autor(a) principal: Bacci, Marcelo Rodrigues
• Data de Publicação: 2017
• Outros Autores: Aguiar Alves, Beatriz da Costa, Cavallari, Marina Romera, Azzalis, Ligia Ajaime [UNIFESP], de Rozier-Alves, Ross Martin, Perez, Matheus Moreira, Chehter, Ethel Zimberg, Pereira, Edimar Cristiano [UNIFESP], Affonso Fonseca, Fernando Luiz [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng; por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S1679-45082017AO4012; https://repositorio.unifesp.br/handle/11600/57175
• topic: Beta-trace protein; Prostaglandins D/biosynthesis; Diabetes mellitus, type 2; Gene expression; Biomarkers/urine; Proteína beta-traço; Prostaglandinas D/biossíntese; Diabetes mellitus tipo 2; Expressão gênica; Biomarcadores/urina

Objective: To evaluate the gene expression of beta-trace protein in urine of diabetic patients, with no reduction in glomerular filtration rate, which was defined as below 60mL/min/1.73m(2). Methods: Type 2 diabetes mellitus patients were recruited, and a group of non-diabetic individuals served as control. Beta-trace protein gene expression was analyzed by quantitative PCR. Blood samples were collected to establish glucose levels and baseline kidney function. Accuracy was analyzed using ROC curves. Results: Ninety type 2 diabetes mellitus patients and 20 non-diabetic individuals were recruited. The area under the curve was 0.601, sensitivity of 20% and specificity of 89.47%. Among diabetic participants, 18% showed an expression above the cutoff point. Conclusion: These results of accuracy of beta-trace protein gene expression in urine of diabetic patients are promising, although they did not achieve a higher area under the curve level.