Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus

Detalhes bibliográficos
Autor(a) principal: Vieira, Michele Juliane [UNIFESP]
Data de Publicação: 2014
Outros Autores: Perosa, Sandra Regina [UNIFESP], Argañaraz, Gustavo Adolfo [UNIFESP], Silva Junior, Jose Antonio [UNIFESP], Cavalheiro, Esper Abrão [UNIFESP], Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.6061/clinics/2014(09)08
http://repositorio.unifesp.br/handle/11600/8599
Resumo: OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules.METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry.RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin.CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
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spelling Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticusIndomethacinInflammatory MediatorsEpilepsyOBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules.METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry.RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin.CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.Universidade Federal de São Paulo (UNIFESP) Departamento de Neurologia/NeurocirurgiaSociedade Beneficente Israelita Brasileira Hospital Albert Einstein Neurofisiologia ClínicaUniversidade de Brasília Faculdade de Ciências da Saúde Laboratório de Patologia MolecularUniversidade Nove de Julho Departamento de Educação Física Programa de Pós-Graduação em Ciências da ReabilitaçãoUniversidade Federal de São Paulo (UNIFESP) Departamento de BioquímicaUNIFESP, Depto. de Neurologia/NeurocirurgiaUNIFESP, Depto. de BioquímicaSciELOFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ)Institutos Nacionais de Ciencia e Tecnologia (INCT)Programa de Apoio a Nucleos de Excelencia (PRONEX)Faculdade de Medicina / USPUniversidade Federal de São Paulo (UNIFESP)Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein Neurofisiologia ClínicaUniversidade de Brasília Faculdade de Ciências da Saúde Laboratório de Patologia MolecularUniversidade Nove de Julho Departamento de Educação Física Programa de Pós-Graduação em Ciências da ReabilitaçãoVieira, Michele Juliane [UNIFESP]Perosa, Sandra Regina [UNIFESP]Argañaraz, Gustavo Adolfo [UNIFESP]Silva Junior, Jose Antonio [UNIFESP]Cavalheiro, Esper Abrão [UNIFESP]Naffah-Mazzacoratti, Maria da Graca [UNIFESP]2015-06-14T13:47:18Z2015-06-14T13:47:18Z2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion621-626application/pdfhttp://dx.doi.org/10.6061/clinics/2014(09)08Clinics. Faculdade de Medicina / USP, v. 69, n. 9, p. 621-626, 2014.10.6061/clinics/2014(09)08S1807-59322014000900621.pdf1807-59321980-5322S1807-59322014000900621http://repositorio.unifesp.br/handle/11600/8599WOS:000343983100008engClinicsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T10:06:51Zoai:repositorio.unifesp.br/:11600/8599Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T10:06:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
spellingShingle Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
Vieira, Michele Juliane [UNIFESP]
Indomethacin
Inflammatory Mediators
Epilepsy
title_short Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_full Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_fullStr Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_full_unstemmed Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
title_sort Indomethacin can downregulate the levels of inflammatory mediators in the hippocampus of rats submitted to pilocarpine-induced status epilepticus
author Vieira, Michele Juliane [UNIFESP]
author_facet Vieira, Michele Juliane [UNIFESP]
Perosa, Sandra Regina [UNIFESP]
Argañaraz, Gustavo Adolfo [UNIFESP]
Silva Junior, Jose Antonio [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
author_role author
author2 Perosa, Sandra Regina [UNIFESP]
Argañaraz, Gustavo Adolfo [UNIFESP]
Silva Junior, Jose Antonio [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Sociedade Beneficente Israelita Brasileira Hospital Albert Einstein Neurofisiologia Clínica
Universidade de Brasília Faculdade de Ciências da Saúde Laboratório de Patologia Molecular
Universidade Nove de Julho Departamento de Educação Física Programa de Pós-Graduação em Ciências da Reabilitação
dc.contributor.author.fl_str_mv Vieira, Michele Juliane [UNIFESP]
Perosa, Sandra Regina [UNIFESP]
Argañaraz, Gustavo Adolfo [UNIFESP]
Silva Junior, Jose Antonio [UNIFESP]
Cavalheiro, Esper Abrão [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
dc.subject.por.fl_str_mv Indomethacin
Inflammatory Mediators
Epilepsy
topic Indomethacin
Inflammatory Mediators
Epilepsy
description OBJECTIVE: Refractory status epilepticus is one of the most life-threatening neurological emergencies and is characterized by high morbidity and mortality. Additionally, the use of anti-inflammatory drugs during this period is very controversial. Thus, this study has been designed to analyze the effect of a low dose of indomethacin (a COX inhibitor) on the expression of inflammatory molecules.METHOD: The hippocampus of rats submitted to pilocarpine-induced long-lasting status epilepticus was analyzed to determine the expression of inflammatory molecules with RT-PCR and immunohistochemistry.RESULTS: Compared with controls, reduced levels of the kinin B2 receptors IL1β and TNFα were found in the hippocampus of rats submitted to long-lasting status epilepticus and treated with indomethacin.CONCLUSIONS: These data show that low doses of indomethacin could be employed to minimize inflammation during long-lasting status epilepticus.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
2015-06-14T13:47:18Z
2015-06-14T13:47:18Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.6061/clinics/2014(09)08
Clinics. Faculdade de Medicina / USP, v. 69, n. 9, p. 621-626, 2014.
10.6061/clinics/2014(09)08
S1807-59322014000900621.pdf
1807-5932
1980-5322
S1807-59322014000900621
http://repositorio.unifesp.br/handle/11600/8599
WOS:000343983100008
url http://dx.doi.org/10.6061/clinics/2014(09)08
http://repositorio.unifesp.br/handle/11600/8599
identifier_str_mv Clinics. Faculdade de Medicina / USP, v. 69, n. 9, p. 621-626, 2014.
10.6061/clinics/2014(09)08
S1807-59322014000900621.pdf
1807-5932
1980-5322
S1807-59322014000900621
WOS:000343983100008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 621-626
application/pdf
dc.publisher.none.fl_str_mv Faculdade de Medicina / USP
publisher.none.fl_str_mv Faculdade de Medicina / USP
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268343631216640

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