Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis

Detalhes bibliográficos
Autor(a) principal: Leme, Marcelo Betim Paes
Data de Publicação: 2006
Outros Autores: Waitzberg, Angela Flávia Logullo [UNIFESP], Artigiani Neto, Ricardo [UNIFESP], Linhares, Marcelo Moura [UNIFESP], Matos, Delcio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502006000600007
http://repositorio.unifesp.br/handle/11600/3387
Resumo: PURPOSE: To quantify the degree of angiogenesis by conventional method (microvessel density, MVD) and computerized method (endothelial area, EA), and to evaluate their relationships with the prognosis of patients operated on for colorectal adenocarcinoma. METHODS: Tumoral angiogenesis was studied by means of an immunohistochemical technique, using CD 34, on 126 patients; to quantify the angiogenesis, MVD (defined as number of microvessels per mm²) and EA measurement (defined as the area occupied by EA in the microscope field). A computerized method, IMAGELab software was utilized to quantify endothelial area. RESULTS: The mean number of microvessels was 128.6 MV/mm² (SD = 44.5) and the mean EA was 4.3% (SD = 2.1). The Pearson method demonstrated a low correlation coefficient between MVD and EA (r = 0.429). No relationship between MVD and EA was observed with regard to relapse-free interval and overall survival. CONCLUSION: The histological analysis of angiogenesis expression in patients with colorectal adenocarcinoma can be performed either by computer-assisted image analysis of endothelial area or by conventional microvessels counting. Both methods did not show any significant relationship between these angiogenesis parameters with relapse-free interval and overall survival.
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spelling Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysisInvestigação da expressão da angiogênese e sua relação com o prognóstico do câncer colorretal pela análise convencional e informatizada da imagem histopatológicaColonic NeoplasmsImage Processing, Computer-AssistedPrognosisNeoplasias do ColoProcessamento de Imagem Assistida por ComputadorPrognósticoPURPOSE: To quantify the degree of angiogenesis by conventional method (microvessel density, MVD) and computerized method (endothelial area, EA), and to evaluate their relationships with the prognosis of patients operated on for colorectal adenocarcinoma. METHODS: Tumoral angiogenesis was studied by means of an immunohistochemical technique, using CD 34, on 126 patients; to quantify the angiogenesis, MVD (defined as number of microvessels per mm²) and EA measurement (defined as the area occupied by EA in the microscope field). A computerized method, IMAGELab software was utilized to quantify endothelial area. RESULTS: The mean number of microvessels was 128.6 MV/mm² (SD = 44.5) and the mean EA was 4.3% (SD = 2.1). The Pearson method demonstrated a low correlation coefficient between MVD and EA (r = 0.429). No relationship between MVD and EA was observed with regard to relapse-free interval and overall survival. CONCLUSION: The histological analysis of angiogenesis expression in patients with colorectal adenocarcinoma can be performed either by computer-assisted image analysis of endothelial area or by conventional microvessels counting. Both methods did not show any significant relationship between these angiogenesis parameters with relapse-free interval and overall survival.OBJETIVO: Quantificar a intensidade da angiogênese pelo método convencional (densidade microvasal, DMV) e pelo método informatizado, área endothelial (AE) e avaliar a sua correlação com o prognóstico de doentes operados por adenocarcinoma colorretal. MÉTODOS: A angiogênese tumoral foi investigada por meio de técnica imuno-histoquímica, utilizando-se CD-34, em 126 doentes; para quantificar a angiogênese, a microdensidade vascular, definida como o número de microvasos por mm² e a medida da área endotelial, definida como a área ocupada pelo endotélio vascular identificada no campo microscópico foram empregadas. Um programa computadorizado, o IMAGELab foi empregado para a quantificação da área endothelial. RESULTADOS: A media do número de microvasos foi de 128,6 MV/mm ² (d esvio padrão de 44,5). O método do coeficiente de Pearson demonstrou uma baixa correlação entre a DMV e a AE (r=0,429). Nenhuma correlação entre a DMV e AE com o intervalo livre de doença e tempo global de sobrevida foi observada. CONCLUSÃO: A análise histológica da expressão angiogênica em doentes com adenocarcinoma colorretal pode ser realizada tanto da forma informatizada, na quantificação da área endotelial, como pela convencional, na contagem dos microvasos. Ambos métodos não demonstraram relação estatisticamente significante entre estes parâmetros de angiogênese e o intervalo livre de doença e a sobrevida global.University Foundation Osvaldo Aranha Department of SurgeryUniversity Center of Volta RedondaUniversidade Federal de São Paulo (UNIFESP) Department of PathologyUNIFESP Pathology Post-Graduate ProgramUNIFESP Gastroenterological Surgery DivisionUNIFESP Gastroenterological Surgery Division Post-Graduate ProgramUNIFESP, Department of PathologyUNIFESP, Pathology Post-Graduate ProgramUNIFESP, Gastroenterological Surgery DivisionUNIFESP, Gastroenterological Surgery Division Post-Graduate ProgramSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversity Foundation Osvaldo Aranha Department of SurgeryUniversity Center of Volta RedondaUniversidade Federal de São Paulo (UNIFESP)Leme, Marcelo Betim PaesWaitzberg, Angela Flávia Logullo [UNIFESP]Artigiani Neto, Ricardo [UNIFESP]Linhares, Marcelo Moura [UNIFESP]Matos, Delcio [UNIFESP]2015-06-14T13:36:34Z2015-06-14T13:36:34Z2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion392-397application/pdfhttp://dx.doi.org/10.1590/S0102-86502006000600007Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 6, p. 392-397, 2006.10.1590/S0102-86502006000600007S0102-86502006000600007.pdf0102-8650S0102-86502006000600007http://repositorio.unifesp.br/handle/11600/3387engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T18:14:17Zoai:repositorio.unifesp.br/:11600/3387Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T18:14:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
Investigação da expressão da angiogênese e sua relação com o prognóstico do câncer colorretal pela análise convencional e informatizada da imagem histopatológica
title Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
spellingShingle Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
Leme, Marcelo Betim Paes
Colonic Neoplasms
Image Processing, Computer-Assisted
Prognosis
Neoplasias do Colo
Processamento de Imagem Assistida por Computador
Prognóstico
title_short Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
title_full Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
title_fullStr Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
title_full_unstemmed Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
title_sort Assessment of angiogenesis expression and its relationship with prognosis of colorectal cancer by conventional and computer-assisted histopathological image analysis
author Leme, Marcelo Betim Paes
author_facet Leme, Marcelo Betim Paes
Waitzberg, Angela Flávia Logullo [UNIFESP]
Artigiani Neto, Ricardo [UNIFESP]
Linhares, Marcelo Moura [UNIFESP]
Matos, Delcio [UNIFESP]
author_role author
author2 Waitzberg, Angela Flávia Logullo [UNIFESP]
Artigiani Neto, Ricardo [UNIFESP]
Linhares, Marcelo Moura [UNIFESP]
Matos, Delcio [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv University Foundation Osvaldo Aranha Department of Surgery
University Center of Volta Redonda
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Leme, Marcelo Betim Paes
Waitzberg, Angela Flávia Logullo [UNIFESP]
Artigiani Neto, Ricardo [UNIFESP]
Linhares, Marcelo Moura [UNIFESP]
Matos, Delcio [UNIFESP]
dc.subject.por.fl_str_mv Colonic Neoplasms
Image Processing, Computer-Assisted
Prognosis
Neoplasias do Colo
Processamento de Imagem Assistida por Computador
Prognóstico
topic Colonic Neoplasms
Image Processing, Computer-Assisted
Prognosis
Neoplasias do Colo
Processamento de Imagem Assistida por Computador
Prognóstico
description PURPOSE: To quantify the degree of angiogenesis by conventional method (microvessel density, MVD) and computerized method (endothelial area, EA), and to evaluate their relationships with the prognosis of patients operated on for colorectal adenocarcinoma. METHODS: Tumoral angiogenesis was studied by means of an immunohistochemical technique, using CD 34, on 126 patients; to quantify the angiogenesis, MVD (defined as number of microvessels per mm²) and EA measurement (defined as the area occupied by EA in the microscope field). A computerized method, IMAGELab software was utilized to quantify endothelial area. RESULTS: The mean number of microvessels was 128.6 MV/mm² (SD = 44.5) and the mean EA was 4.3% (SD = 2.1). The Pearson method demonstrated a low correlation coefficient between MVD and EA (r = 0.429). No relationship between MVD and EA was observed with regard to relapse-free interval and overall survival. CONCLUSION: The histological analysis of angiogenesis expression in patients with colorectal adenocarcinoma can be performed either by computer-assisted image analysis of endothelial area or by conventional microvessels counting. Both methods did not show any significant relationship between these angiogenesis parameters with relapse-free interval and overall survival.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
2015-06-14T13:36:34Z
2015-06-14T13:36:34Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502006000600007
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 6, p. 392-397, 2006.
10.1590/S0102-86502006000600007
S0102-86502006000600007.pdf
0102-8650
S0102-86502006000600007
http://repositorio.unifesp.br/handle/11600/3387
url http://dx.doi.org/10.1590/S0102-86502006000600007
http://repositorio.unifesp.br/handle/11600/3387
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 6, p. 392-397, 2006.
10.1590/S0102-86502006000600007
S0102-86502006000600007.pdf
0102-8650
S0102-86502006000600007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 392-397
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268412903292928

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