Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/6643 http://dx.doi.org/10.1590/S0100-879X2011007500105 |
Resumo: | Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs. |
id |
UFSP_781659d3e4d13ed4f193dbdf2b831b49 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/6643 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Liberatore, A.m.a. [UNIFESP]Moreira, F.c. [UNIFESP]Gomes, Tania Aparecida Tardelli [UNIFESP]Díaz, José Luis Menchaca [UNIFESP]Koh, Ivan Hong Jun [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:43:17Z2015-06-14T13:43:17Z2011-10-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 10, p. 1018-1024, 2011.0100-879Xhttp://repositorio.unifesp.br/handle/11600/6643http://dx.doi.org/10.1590/S0100-879X2011007500105S0100-879X2011001000007.pdfS0100-879X201100100000710.1590/S0100-879X2011007500105WOS:000295721600007Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs.Universidade Federal de São Paulo (UNIFESP) Departamento de PediatriaUniversidade Federal de São Paulo (UNIFESP) Departamento de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP) Departamento de CirurgiaUNIFESP, Depto. de PediatriaUNIFESP, Depto. de MicrobiologiaUNIFESP, Depto. de CirurgiaSciELO1018-1024engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchEnteropathogenic Escherichia coli (EPEC)Atypical EPECBacterial translocationMicrocirculationTissue perfusionTypical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2011001000007.pdfapplication/pdf2898385${dspace.ui.url}/bitstream/11600/6643/1/S0100-879X2011001000007.pdfaecfa884b670fb117b58b67f53a4e8b3MD51open accessTEXTS0100-879X2011001000007.pdf.txtS0100-879X2011001000007.pdf.txtExtracted texttext/plain28171${dspace.ui.url}/bitstream/11600/6643/21/S0100-879X2011001000007.pdf.txt6a46f48a99321340d03b7cdbf71a3717MD521open accessTHUMBNAILS0100-879X2011001000007.pdf.jpgS0100-879X2011001000007.pdf.jpgIM Thumbnailimage/jpeg9600${dspace.ui.url}/bitstream/11600/6643/23/S0100-879X2011001000007.pdf.jpged72c8461d940acee5017681546c5212MD523open access11600/66432023-06-05 19:37:05.84open accessoai:repositorio.unifesp.br:11600/6643Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:37:05Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
title |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
spellingShingle |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats Liberatore, A.m.a. [UNIFESP] Enteropathogenic Escherichia coli (EPEC) Atypical EPEC Bacterial translocation Microcirculation Tissue perfusion |
title_short |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
title_full |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
title_fullStr |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
title_full_unstemmed |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
title_sort |
Typical and atypical enteropathogenic Escherichia coli bacterial translocation associated with tissue hypoperfusion in rats |
author |
Liberatore, A.m.a. [UNIFESP] |
author_facet |
Liberatore, A.m.a. [UNIFESP] Moreira, F.c. [UNIFESP] Gomes, Tania Aparecida Tardelli [UNIFESP] Díaz, José Luis Menchaca [UNIFESP] Koh, Ivan Hong Jun [UNIFESP] |
author_role |
author |
author2 |
Moreira, F.c. [UNIFESP] Gomes, Tania Aparecida Tardelli [UNIFESP] Díaz, José Luis Menchaca [UNIFESP] Koh, Ivan Hong Jun [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Liberatore, A.m.a. [UNIFESP] Moreira, F.c. [UNIFESP] Gomes, Tania Aparecida Tardelli [UNIFESP] Díaz, José Luis Menchaca [UNIFESP] Koh, Ivan Hong Jun [UNIFESP] |
dc.subject.eng.fl_str_mv |
Enteropathogenic Escherichia coli (EPEC) Atypical EPEC Bacterial translocation Microcirculation Tissue perfusion |
topic |
Enteropathogenic Escherichia coli (EPEC) Atypical EPEC Bacterial translocation Microcirculation Tissue perfusion |
description |
Although enteropathogenic Escherichia coli (EPEC) are well-recognized diarrheal agents, their ability to translocate and cause extraintestinal alterations is not known. We investigated whether a typical EPEC (tEPEC) and an atypical EPEC (aEPEC) strain translocate and cause microcirculation injury under conditions of intestinal bacterial overgrowth. Bacterial translocation (BT) was induced in female Wistar-EPM rats (200-250 g) by oroduodenal catheterization and inoculation of 10 mL 10(10) colony forming unit (CFU)/mL, with the bacteria being confined between the duodenum and ileum with ligatures. After 2 h, mesenteric lymph nodes (MLN), liver and spleen were cultured for translocated bacteria and BT-related microcirculation changes were monitored in mesenteric and abdominal organs by intravital microscopy and laser Doppler flow, respectively. tEPEC (N = 11) and aEPEC (N = 11) were recovered from MLN (100%), spleen (36.4 and 45.5%), and liver (45.5 and 72.7%) of the animals, respectively. Recovery of the positive control E. coli R-6 (N = 6) was 100% for all compartments. Bacteria were not recovered from extraintestinal sites of controls inoculated with non-pathogenic E. coli strains HB101 (N = 6) and HS (N = 10), or saline. Mesenteric microcirculation injuries were detected with both EPEC strains, but only aEPEC was similar to E. coli R-6 with regard to systemic tissue hypoperfusion. In conclusion, overgrowth of certain aEPEC strains may lead to BT and impairment of the microcirculation in systemic organs. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:43:17Z |
dc.date.available.fl_str_mv |
2015-06-14T13:43:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 10, p. 1018-1024, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/6643 http://dx.doi.org/10.1590/S0100-879X2011007500105 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2011001000007.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2011001000007 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2011007500105 |
dc.identifier.wos.none.fl_str_mv |
WOS:000295721600007 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 44, n. 10, p. 1018-1024, 2011. 0100-879X S0100-879X2011001000007.pdf S0100-879X2011001000007 10.1590/S0100-879X2011007500105 WOS:000295721600007 |
url |
http://repositorio.unifesp.br/handle/11600/6643 http://dx.doi.org/10.1590/S0100-879X2011007500105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1018-1024 |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/6643/1/S0100-879X2011001000007.pdf ${dspace.ui.url}/bitstream/11600/6643/21/S0100-879X2011001000007.pdf.txt ${dspace.ui.url}/bitstream/11600/6643/23/S0100-879X2011001000007.pdf.jpg |
bitstream.checksum.fl_str_mv |
aecfa884b670fb117b58b67f53a4e8b3 6a46f48a99321340d03b7cdbf71a3717 ed72c8461d940acee5017681546c5212 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764197875941376 |