Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress

Detalhes bibliográficos
Autor(a) principal: Punaro, Giovana Rita [UNIFESP]
Data de Publicação: 2014
Outros Autores: Maciel, Fabiane Romano [UNIFESP], Rodrigues, Adelson Marçal [UNIFESP], Rogero, Marcelo Macedo, Bogsan, Cristina Stewart Bittencourt, Oliveira, Marice Nogueira de, Ihara, Silvia Saiuli Miki [UNIFESP], Araujo, Sergio R. R. [UNIFESP], Sanches, Talita Rojas Cunha, Andrade, Lucia C., Higa, Elisa Mieko Suemitsu [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.niox.2013.12.012
http://repositorio.unifesp.br/handle/11600/37439
Resumo: This study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved.
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spelling Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stressKefirOxidative stressNitric oxideDiabetes and renal functionThis study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved.Universidade Federal de São Paulo, Dept Med, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, BrazilUniv São Paulo, Coll Publ Hlth, Dept Nutr, São Paulo, BrazilUniv São Paulo, Dept Biochem & Pharmaceut Technol, São Paulo, BrazilUniv São Paulo, Dept Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Apoio a Universidade Federal de São Paulo (FAP-Unifesp)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Punaro, Giovana Rita [UNIFESP]Maciel, Fabiane Romano [UNIFESP]Rodrigues, Adelson Marçal [UNIFESP]Rogero, Marcelo MacedoBogsan, Cristina Stewart BittencourtOliveira, Marice Nogueira deIhara, Silvia Saiuli Miki [UNIFESP]Araujo, Sergio R. R. [UNIFESP]Sanches, Talita Rojas CunhaAndrade, Lucia C.Higa, Elisa Mieko Suemitsu [UNIFESP]2016-01-24T14:35:19Z2016-01-24T14:35:19Z2014-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion53-60application/pdfhttp://dx.doi.org/10.1016/j.niox.2013.12.012Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014.10.1016/j.niox.2013.12.012WOS000333139200007.pdf1089-8603http://repositorio.unifesp.br/handle/11600/37439WOS:000333139200007engNitric Oxide-biology and Chemistryinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T05:55:20Zoai:repositorio.unifesp.br/:11600/37439Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T05:55:20Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
title Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
spellingShingle Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
Punaro, Giovana Rita [UNIFESP]
Kefir
Oxidative stress
Nitric oxide
Diabetes and renal function
title_short Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
title_full Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
title_fullStr Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
title_full_unstemmed Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
title_sort Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
author Punaro, Giovana Rita [UNIFESP]
author_facet Punaro, Giovana Rita [UNIFESP]
Maciel, Fabiane Romano [UNIFESP]
Rodrigues, Adelson Marçal [UNIFESP]
Rogero, Marcelo Macedo
Bogsan, Cristina Stewart Bittencourt
Oliveira, Marice Nogueira de
Ihara, Silvia Saiuli Miki [UNIFESP]
Araujo, Sergio R. R. [UNIFESP]
Sanches, Talita Rojas Cunha
Andrade, Lucia C.
Higa, Elisa Mieko Suemitsu [UNIFESP]
author_role author
author2 Maciel, Fabiane Romano [UNIFESP]
Rodrigues, Adelson Marçal [UNIFESP]
Rogero, Marcelo Macedo
Bogsan, Cristina Stewart Bittencourt
Oliveira, Marice Nogueira de
Ihara, Silvia Saiuli Miki [UNIFESP]
Araujo, Sergio R. R. [UNIFESP]
Sanches, Talita Rojas Cunha
Andrade, Lucia C.
Higa, Elisa Mieko Suemitsu [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Punaro, Giovana Rita [UNIFESP]
Maciel, Fabiane Romano [UNIFESP]
Rodrigues, Adelson Marçal [UNIFESP]
Rogero, Marcelo Macedo
Bogsan, Cristina Stewart Bittencourt
Oliveira, Marice Nogueira de
Ihara, Silvia Saiuli Miki [UNIFESP]
Araujo, Sergio R. R. [UNIFESP]
Sanches, Talita Rojas Cunha
Andrade, Lucia C.
Higa, Elisa Mieko Suemitsu [UNIFESP]
dc.subject.por.fl_str_mv Kefir
Oxidative stress
Nitric oxide
Diabetes and renal function
topic Kefir
Oxidative stress
Nitric oxide
Diabetes and renal function
description This study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-15
2016-01-24T14:35:19Z
2016-01-24T14:35:19Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.niox.2013.12.012
Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014.
10.1016/j.niox.2013.12.012
WOS000333139200007.pdf
1089-8603
http://repositorio.unifesp.br/handle/11600/37439
WOS:000333139200007
url http://dx.doi.org/10.1016/j.niox.2013.12.012
http://repositorio.unifesp.br/handle/11600/37439
identifier_str_mv Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014.
10.1016/j.niox.2013.12.012
WOS000333139200007.pdf
1089-8603
WOS:000333139200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nitric Oxide-biology and Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 53-60
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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