Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.niox.2013.12.012 http://repositorio.unifesp.br/handle/11600/37439 |
Resumo: | This study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved. |
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Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stressKefirOxidative stressNitric oxideDiabetes and renal functionThis study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved.Universidade Federal de São Paulo, Dept Med, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, BrazilUniv São Paulo, Coll Publ Hlth, Dept Nutr, São Paulo, BrazilUniv São Paulo, Dept Biochem & Pharmaceut Technol, São Paulo, BrazilUniv São Paulo, Dept Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Apoio a Universidade Federal de São Paulo (FAP-Unifesp)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Punaro, Giovana Rita [UNIFESP]Maciel, Fabiane Romano [UNIFESP]Rodrigues, Adelson Marçal [UNIFESP]Rogero, Marcelo MacedoBogsan, Cristina Stewart BittencourtOliveira, Marice Nogueira deIhara, Silvia Saiuli Miki [UNIFESP]Araujo, Sergio R. R. [UNIFESP]Sanches, Talita Rojas CunhaAndrade, Lucia C.Higa, Elisa Mieko Suemitsu [UNIFESP]2016-01-24T14:35:19Z2016-01-24T14:35:19Z2014-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion53-60application/pdfhttp://dx.doi.org/10.1016/j.niox.2013.12.012Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014.10.1016/j.niox.2013.12.012WOS000333139200007.pdf1089-8603http://repositorio.unifesp.br/handle/11600/37439WOS:000333139200007engNitric Oxide-biology and Chemistryinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T05:55:20Zoai:repositorio.unifesp.br/:11600/37439Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T05:55:20Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
title |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
spellingShingle |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress Punaro, Giovana Rita [UNIFESP] Kefir Oxidative stress Nitric oxide Diabetes and renal function |
title_short |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
title_full |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
title_fullStr |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
title_full_unstemmed |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
title_sort |
Kefir administration reduced progression of renal injury in STZ-diabetic rats by lowering oxidative stress |
author |
Punaro, Giovana Rita [UNIFESP] |
author_facet |
Punaro, Giovana Rita [UNIFESP] Maciel, Fabiane Romano [UNIFESP] Rodrigues, Adelson Marçal [UNIFESP] Rogero, Marcelo Macedo Bogsan, Cristina Stewart Bittencourt Oliveira, Marice Nogueira de Ihara, Silvia Saiuli Miki [UNIFESP] Araujo, Sergio R. R. [UNIFESP] Sanches, Talita Rojas Cunha Andrade, Lucia C. Higa, Elisa Mieko Suemitsu [UNIFESP] |
author_role |
author |
author2 |
Maciel, Fabiane Romano [UNIFESP] Rodrigues, Adelson Marçal [UNIFESP] Rogero, Marcelo Macedo Bogsan, Cristina Stewart Bittencourt Oliveira, Marice Nogueira de Ihara, Silvia Saiuli Miki [UNIFESP] Araujo, Sergio R. R. [UNIFESP] Sanches, Talita Rojas Cunha Andrade, Lucia C. Higa, Elisa Mieko Suemitsu [UNIFESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Punaro, Giovana Rita [UNIFESP] Maciel, Fabiane Romano [UNIFESP] Rodrigues, Adelson Marçal [UNIFESP] Rogero, Marcelo Macedo Bogsan, Cristina Stewart Bittencourt Oliveira, Marice Nogueira de Ihara, Silvia Saiuli Miki [UNIFESP] Araujo, Sergio R. R. [UNIFESP] Sanches, Talita Rojas Cunha Andrade, Lucia C. Higa, Elisa Mieko Suemitsu [UNIFESP] |
dc.subject.por.fl_str_mv |
Kefir Oxidative stress Nitric oxide Diabetes and renal function |
topic |
Kefir Oxidative stress Nitric oxide Diabetes and renal function |
description |
This study aimed at assessing the effects of Kefir, a probiotic fermented milk, on oxidative stress in diabetic animals. the induction of diabetes was achieved in adult male Wistar rats using streptozotocin (STZ). the animals were distributed into four groups as follows: control (CTL); control Kefir (CTLK); diabetic (DM) and diabetic Kefir (DMK). Starting on the 5th day of diabetes, Kefir was administered by daily gavage at a dose of 1.8 mL/day for 8 weeks. Before and after Kefir treatment, the rats were placed in individual metabolic cages to obtain blood and urine samples to evaluate urea, creatinine, proteinuria, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and C-reactive protein (CRP). After sacrificing the animals, the renal cortex was removed for histology, oxidative stress and NOS evaluation. When compared to CTL rats, DM rats showed increased levels of glycemia, plasmatic urea, proteinuria, renal NO, superoxide anion, TBARS, and plasmatic CRP; also demonstrated a reduction in urinary urea, creatinine, and NO. However, DMK rats showed a significant improvement in most of these parameters. Despite the lack of differences observed in the expression of endothelial NO synthase (eNOS), the expression of inducible NO synthase (iNOS) was significantly lower in the DMK group when compared to DM rats, as assessed by Western blot analysis. Moreover, the DMK group presented a significant reduction of glycogen accumulation within the renal tubules when compared to the DM group. These results indicate that Kefir treatment may contribute to better control of glycemia and oxidative stress, which is associated with the amelioration of renal function, suggesting its use as a non-pharmacological adjuvant to delay the progression of diabetic complications. (C) 2014 Elsevier Inc. All rights reserved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-15 2016-01-24T14:35:19Z 2016-01-24T14:35:19Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.niox.2013.12.012 Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014. 10.1016/j.niox.2013.12.012 WOS000333139200007.pdf 1089-8603 http://repositorio.unifesp.br/handle/11600/37439 WOS:000333139200007 |
url |
http://dx.doi.org/10.1016/j.niox.2013.12.012 http://repositorio.unifesp.br/handle/11600/37439 |
identifier_str_mv |
Nitric Oxide-biology and Chemistry. San Diego: Academic Press Inc Elsevier Science, v. 37, p. 53-60, 2014. 10.1016/j.niox.2013.12.012 WOS000333139200007.pdf 1089-8603 WOS:000333139200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nitric Oxide-biology and Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
53-60 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268361876439040 |