Estudo das alterações moleculares no plasma seminal associadas ao tabagismo

• Autor(a) principal: Antoniassi, Mariana Pereira [UNIFESP]
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300001443c
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7289666; https://repositorio.unifesp.br/handle/11600/52883
• topic: Smoking; Male infertility; Inflammation; Proteins; Heat-shock proteins; Tabagismo; Infertilidade masculina; Inflamação; Proteínas; Heat-shock proteins

Objective: The composition of seminal plasma may reflect significant testicular changes caused by smoking. Thus, the analysis of the protein composition of the seminal plasma becomes a resource to evaluate these alterations. Therefore, the objetive of this study was to evaluate seminal plasma proteins in smokers related to inflammation, inflammasome complex and heatshock proteins. Methods: This study was divided into three studies. In study 1, seminal samples from 59 controls and 39 smokers were used. After semen collection and liquefaction, an aliquot was used for the seminal analysis and the remaining volume was centrifuged for seminal plasma separation. Seminal plasma was then used to evaluate the seminal levels of the proteins suggested, by Western blotting: S100A9 for the smoking group and SCGB2A1 for the control group. In study 2, seminal samples from 37 control patients and 41 smokers were used. After semen collection and liquefaction, an aliquot was used for the seminal analysis and the remaining volume was centrifuged for seminal plasma separation. Seminal plasma was then utilized to evaluate the seminal levels of the inflammasome complex proteins by Western blotting (ASC, NLRP3, Pannexin1, P2X7) and cytokines by ELISA (Caspase1, IL18 and IL1β). In study 3, seminal samples from 30 control patients and 31 smokers were used. After semen collection and liquefaction, an aliquot was used for the seminal analysis and the remaining volume was centrifuged for seminal plasma separation. Seminal plasma was then utilized to evaluate the expression of heat shock proteins (HSP) by the Multiplex assay. Results: In study 1, seminal levels of S100A9 and SCGB2A1 proteins was increased in the smoking group. In study 2, seminal levels of ASC protein and IL18 cytokine was increased in the smoking group. The NLRP3, Pannexin1, P2X7 proteins, as well as the Caspase1 and IL1β cytokines did not differ between the groups. In study 3, the smoking group showed a decrease in the seminal levels of HSP 90 alpha. Conclusion: The seminal plasma proteins of smokers reflect the inflammatory profile present in these patients.