Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil

Detalhes bibliográficos
Autor(a) principal: Herkert, Patricia Fernanda
Data de Publicação: 2018
Outros Autores: Meis, Jacques F., Salvador, Gabriel Lucca de Oliveira, Gomes, Renata Rodrigues, Vicente, Vania Aparecida, Muro, Marisol Dominguez, Pinheiro, Rosangela Lameira, Colombo, Arnaldo Lopes [UNIFESP], Schwarzbold, Alexandre Vargas, Oliveira, Carla Sakuma de, Ferreira, Marcelo Simao, Queiroz-Telles, Flavio, Hagen, Ferry
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1099/jmm.0.000698
https://repositorio.unifesp.br/handle/11600/55714
Resumo: Purpose. Cryptococcosis is acquired from the environment by the inhalation of Cryptococcus cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a Cryptococcus neoformans species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of C. neoformans sensu lato isolates from southern Brazil. Methodology. We studied 219 C. neoformans sensu lato isolates with mating-and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing. Results/Key findings. Among the isolates, 136 (69 %) were from HIV-positive patients. Only C. neoformans mating-type a and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (n=172
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spelling Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern BrazilCryptococcosisCryptococcus neoformansamplified fragment length polymorphismmicrosatellite typingmating-typeantifungal susceptibilityPurpose. Cryptococcosis is acquired from the environment by the inhalation of Cryptococcus cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a Cryptococcus neoformans species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of C. neoformans sensu lato isolates from southern Brazil. Methodology. We studied 219 C. neoformans sensu lato isolates with mating-and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing. Results/Key findings. Among the isolates, 136 (69 %) were from HIV-positive patients. Only C. neoformans mating-type a and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (n=17278.5 %), AFLP1A/VNII (n=198.7 %), AFLP1B/VNII (n=41.8 %) and a new AFLP pattern AFLP1C (n=2310.5 %). All isolates were susceptible to tested antifungals and no correlation between antifungal susceptibility and genotypes was observed. Through microsatellite analysis, most isolates clustered in a major microsatellite complex and Simpson's diversity index of this population was D=0.9856. Conclusion. The majority of C. neoformans sensu stricto infections occurred in HIV-positive patients. C. neoformans AFLP1/VNI was the most frequent genotype and all antifungal drugs had high in vitro activity against this species. Microsatellite analyses showed a high genetic diversity within the regional C. neoformans sensu stricto population, and correlation between environmental and clinical isolates, as well as a temporal and geographic relationship.Univ Fed Parana, Dept Basic Pathol, Postgrad Program Microbiol Parasitol & Pathol Bio, Curitiba, Parana, BrazilCanisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, NetherlandsCAPES Fdn, Minist Educ Brazil, Brasilia, DF, BrazilCtr Expertise Mycol, Radboudumc CWZ, Nijmegen, NetherlandsUniv Fed Parana, Dept Internal Med, Curitiba, Parana, BrazilState Univ Parana, Dept Biol Sci, Campus Paranagua, Paranagua, PR, BrazilUniv Fed Parana, Hosp Clin, Lab Mycol, Curitiba, Parana, BrazilUniv Fed Sao Paulo, Hosp Sao Paulo, Sao Paulo, BrazilUniv Fed Santa Maria, Hosp Univ, Santa Maria, RS, BrazilUniv Estadual Oeste Parana, Hosp Univ Oeste Parana, Cascavel, BrazilUniv Fed Uberlandia, Uberlandia, MG, BrazilUniv Fed Parana, Hosp Clin, Comunnitarian Hlth Dept, Curitiba, Parana, BrazilWesterdijk Fungal Biodivers Inst, Dept Med Mycol, Utrecht, NetherlandsUniv Fed Sao Paulo, Hosp Sao Paulo, Sao Paulo, BrazilWeb of ScienceCoordenacao de Aperfeicoamento de Pessoal de Nivel SuperiorConselho Nacional de Desenvolvimento Cientifico e Tecnologico, BrazilMicrobiology Soc2020-07-20T16:31:07Z2020-07-20T16:31:07Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion560-569http://dx.doi.org/10.1099/jmm.0.000698Journal Of Medical Microbiology. London, v. 67, n. 4, p. 560-569, 2018.10.1099/jmm.0.0006980022-2615https://repositorio.unifesp.br/handle/11600/55714WOS:000429658500014engJournal Of Medical MicrobiologyLondoninfo:eu-repo/semantics/openAccessHerkert, Patricia FernandaMeis, Jacques F.Salvador, Gabriel Lucca de OliveiraGomes, Renata RodriguesVicente, Vania AparecidaMuro, Marisol DominguezPinheiro, Rosangela LameiraColombo, Arnaldo Lopes [UNIFESP]Schwarzbold, Alexandre VargasOliveira, Carla Sakuma deFerreira, Marcelo SimaoQueiroz-Telles, FlavioHagen, Ferryreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-08T12:05:22Zoai:repositorio.unifesp.br/:11600/55714Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-08T12:05:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
title Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
spellingShingle Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
Herkert, Patricia Fernanda
Cryptococcosis
Cryptococcus neoformans
amplified fragment length polymorphism
microsatellite typing
mating-type
antifungal susceptibility
title_short Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
title_full Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
title_fullStr Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
title_full_unstemmed Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
title_sort Molecular characterization and antifungal susceptibility testing of Cryptococcus neoformans sensu stricto from southern Brazil
author Herkert, Patricia Fernanda
author_facet Herkert, Patricia Fernanda
Meis, Jacques F.
Salvador, Gabriel Lucca de Oliveira
Gomes, Renata Rodrigues
Vicente, Vania Aparecida
Muro, Marisol Dominguez
Pinheiro, Rosangela Lameira
Colombo, Arnaldo Lopes [UNIFESP]
Schwarzbold, Alexandre Vargas
Oliveira, Carla Sakuma de
Ferreira, Marcelo Simao
Queiroz-Telles, Flavio
Hagen, Ferry
author_role author
author2 Meis, Jacques F.
Salvador, Gabriel Lucca de Oliveira
Gomes, Renata Rodrigues
Vicente, Vania Aparecida
Muro, Marisol Dominguez
Pinheiro, Rosangela Lameira
Colombo, Arnaldo Lopes [UNIFESP]
Schwarzbold, Alexandre Vargas
Oliveira, Carla Sakuma de
Ferreira, Marcelo Simao
Queiroz-Telles, Flavio
Hagen, Ferry
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Herkert, Patricia Fernanda
Meis, Jacques F.
Salvador, Gabriel Lucca de Oliveira
Gomes, Renata Rodrigues
Vicente, Vania Aparecida
Muro, Marisol Dominguez
Pinheiro, Rosangela Lameira
Colombo, Arnaldo Lopes [UNIFESP]
Schwarzbold, Alexandre Vargas
Oliveira, Carla Sakuma de
Ferreira, Marcelo Simao
Queiroz-Telles, Flavio
Hagen, Ferry
dc.subject.por.fl_str_mv Cryptococcosis
Cryptococcus neoformans
amplified fragment length polymorphism
microsatellite typing
mating-type
antifungal susceptibility
topic Cryptococcosis
Cryptococcus neoformans
amplified fragment length polymorphism
microsatellite typing
mating-type
antifungal susceptibility
description Purpose. Cryptococcosis is acquired from the environment by the inhalation of Cryptococcus cells and may establish from an asymptomatic latent infection into pneumonia or meningoencephalitis. The genetic diversity of a Cryptococcus neoformans species complex has been investigated by several molecular tools, such as multi-locus sequence typing, amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism and microsatellite analysis. This study aimed to investigate the genotype distributions and antifungal susceptibility profiles of C. neoformans sensu lato isolates from southern Brazil. Methodology. We studied 219 C. neoformans sensu lato isolates with mating-and serotyping, AFLP fingerprinting, microsatellite typing and antifungal susceptibility testing. Results/Key findings. Among the isolates, 136 (69 %) were from HIV-positive patients. Only C. neoformans mating-type a and serotype A were observed. AFLP fingerprinting analysis divided the isolates into AFLP1/VNI (n=172
publishDate 2018
dc.date.none.fl_str_mv 2018
2020-07-20T16:31:07Z
2020-07-20T16:31:07Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1099/jmm.0.000698
Journal Of Medical Microbiology. London, v. 67, n. 4, p. 560-569, 2018.
10.1099/jmm.0.000698
0022-2615
https://repositorio.unifesp.br/handle/11600/55714
WOS:000429658500014
url http://dx.doi.org/10.1099/jmm.0.000698
https://repositorio.unifesp.br/handle/11600/55714
identifier_str_mv Journal Of Medical Microbiology. London, v. 67, n. 4, p. 560-569, 2018.
10.1099/jmm.0.000698
0022-2615
WOS:000429658500014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medical Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 560-569
dc.coverage.none.fl_str_mv London
dc.publisher.none.fl_str_mv Microbiology Soc
publisher.none.fl_str_mv Microbiology Soc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268444212723712

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