Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion

Detalhes bibliográficos
Autor(a) principal: Nasser, José Augusto [UNIFESP]
Data de Publicação: 2006
Outros Autores: Falavigna, Asdrubal [UNIFESP], Ferraz, Fernando [UNIFESP], Duigou, Gregory, Bruce, Jeffrey
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0004-282X2006000500014
http://repositorio.unifesp.br/handle/11600/3245
Resumo: PURPOSE: To evaluate using transcription analysis the presence and importance of two genes: NM23-H1 and TIMP-1 on control of tumor cell invasion in diffuse astrocytomas (WHO II) and glioblastoma multiforme (WHO IV). METHOD: Northern blot analysis of NM23-H1 and TIMP-1 was performed. Eight diffuse astrocytomas and 19 glioblastomas (WHO IV) were analyzed to determine if TIMP-1 and NM23-H1 were candidates to inhibition of tumor cell invasion quantitated RNA levels. The samples were collected directly from operating room. Total cellular RNA was extracted from frozen tissue samples using guanidinium-isothiocyanate and cesium chloride gradients. Total RNA (10 mg per sample) from tumor tissue were size fractionated through 1% agarose-formaldehyde gel and transferred to nylon filters and then hybridized to 32P-labeled DNA probes and placed for autoradiography. Levels of specific RNAs were determined by computer-assisted laser densitometry. Blot filters were sequentially hybridized to nm23 and TIMP-1 probes in addition to GAPDH, as a control. Statistical analyses were carried out according to t-test for equality of means. RESULTS: NM23-H1 were detected in each sample, however it did not correlate with malignancy and invasiveness. On the other side TIMP-1 gene expression showed a clear correlation between low expression and invasiveness. CONCLUSION: The data suggest that TIMP-1 is an inhibitor of high grade gliomas invasion. NM23-H1 was present in the entire gliomas sample, but it did not vary in diffuse astrocytomas and glioblastomas.
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spelling Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasionAnálise transcricional dos genes TIMP-1 e NM23-H1 na invasão celular em astrocitoma difuso e glioblastoma multiformetumor cell invasionTIMP-1NM23-H1RNA levelsgliomasinvasão celular tumoralTIMP-1NM23-H1níveis de RNAgliomasPURPOSE: To evaluate using transcription analysis the presence and importance of two genes: NM23-H1 and TIMP-1 on control of tumor cell invasion in diffuse astrocytomas (WHO II) and glioblastoma multiforme (WHO IV). METHOD: Northern blot analysis of NM23-H1 and TIMP-1 was performed. Eight diffuse astrocytomas and 19 glioblastomas (WHO IV) were analyzed to determine if TIMP-1 and NM23-H1 were candidates to inhibition of tumor cell invasion quantitated RNA levels. The samples were collected directly from operating room. Total cellular RNA was extracted from frozen tissue samples using guanidinium-isothiocyanate and cesium chloride gradients. Total RNA (10 mg per sample) from tumor tissue were size fractionated through 1% agarose-formaldehyde gel and transferred to nylon filters and then hybridized to 32P-labeled DNA probes and placed for autoradiography. Levels of specific RNAs were determined by computer-assisted laser densitometry. Blot filters were sequentially hybridized to nm23 and TIMP-1 probes in addition to GAPDH, as a control. Statistical analyses were carried out according to t-test for equality of means. RESULTS: NM23-H1 were detected in each sample, however it did not correlate with malignancy and invasiveness. On the other side TIMP-1 gene expression showed a clear correlation between low expression and invasiveness. CONCLUSION: The data suggest that TIMP-1 is an inhibitor of high grade gliomas invasion. NM23-H1 was present in the entire gliomas sample, but it did not vary in diffuse astrocytomas and glioblastomas.OBJETIVO: Comparar através da análise da expressão dos níveis de RNA, a presença e a relevância dos genes NM23-H1 e TIMP-1 no controle da invasão celular tumoral dentro do tecido cerebral normal em: astrocitoma difuso (OMS II) e glioblastoma multiforme (OMS:IV). MÉTODO: Análise em Northern blot dos genes NM23-H1 e TIMP-1. Oito astrocitomas fibrilares difusos (OMS II) e 19 glioblastomas multiformes foram analisados para determinar se TIMP-1 e NM23-H1 estavam relacinados à inibição da invasão tumoral nas neoplasias do sistema nervoso central, quantificando os níveis de RNA dos respectivos genes extraídos diretamente dos tumores. 10 mg por amostra de RNA total foram fracionados de gel de formaldeído e transferidos para os filmes de hibridação. Níveis específicos de RNAs foram determinados na espectrofotometria. Valores das razões entre NM23-H1/GAPDH e TIMP-1/GAPDH foram submetidos à análise de variabilidade das médias. RESULTADOS: A análise da expressão do gene TIMP-1 mostrou supressão em tumores gliais malignos. CONCLUSÃO: Os resultados indicam que existe relação direta entre níveis baixos de TIMP-1 e malignidade dos gliomas. O gene NM23-H1 foi detectado em todas as amostras, mas não foi possível relacionar sua subexpressão ou superexpressão com algum fenótipo de invasividade.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaUniversidade Estácio de SáUniversidade Caxias do SulColumbia University College of Physicians and Surgeons Department of NeurosurgeryUNIFESP, EPMSciELOAcademia Brasileira de Neurologia - ABNEUROUniversidade Federal de São Paulo (UNIFESP)Universidade Estácio de SáUniversidade Caxias do SulColumbia University College of Physicians and Surgeons Department of NeurosurgeryNasser, José Augusto [UNIFESP]Falavigna, Asdrubal [UNIFESP]Ferraz, Fernando [UNIFESP]Duigou, GregoryBruce, Jeffrey2015-06-14T13:36:26Z2015-06-14T13:36:26Z2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion774-780application/pdfhttp://dx.doi.org/10.1590/S0004-282X2006000500014Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 64, n. 3b, p. 774-780, 2006.10.1590/S0004-282X2006000500014S0004-282X2006000500014.pdf0004-282XS0004-282X2006000500014http://repositorio.unifesp.br/handle/11600/3245engArquivos de Neuro-Psiquiatriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-10-10T10:29:57Zoai:repositorio.unifesp.br/:11600/3245Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-10-10T10:29:57Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
Análise transcricional dos genes TIMP-1 e NM23-H1 na invasão celular em astrocitoma difuso e glioblastoma multiforme
title Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
spellingShingle Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
Nasser, José Augusto [UNIFESP]
tumor cell invasion
TIMP-1
NM23-H1
RNA levels
gliomas
invasão celular tumoral
TIMP-1
NM23-H1
níveis de RNA
gliomas
title_short Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
title_full Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
title_fullStr Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
title_full_unstemmed Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
title_sort Transcription analysis of TIMP-1 and NM23-h1 genes in glioma cell invasion
author Nasser, José Augusto [UNIFESP]
author_facet Nasser, José Augusto [UNIFESP]
Falavigna, Asdrubal [UNIFESP]
Ferraz, Fernando [UNIFESP]
Duigou, Gregory
Bruce, Jeffrey
author_role author
author2 Falavigna, Asdrubal [UNIFESP]
Ferraz, Fernando [UNIFESP]
Duigou, Gregory
Bruce, Jeffrey
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estácio de Sá
Universidade Caxias do Sul
Columbia University College of Physicians and Surgeons Department of Neurosurgery
dc.contributor.author.fl_str_mv Nasser, José Augusto [UNIFESP]
Falavigna, Asdrubal [UNIFESP]
Ferraz, Fernando [UNIFESP]
Duigou, Gregory
Bruce, Jeffrey
dc.subject.por.fl_str_mv tumor cell invasion
TIMP-1
NM23-H1
RNA levels
gliomas
invasão celular tumoral
TIMP-1
NM23-H1
níveis de RNA
gliomas
topic tumor cell invasion
TIMP-1
NM23-H1
RNA levels
gliomas
invasão celular tumoral
TIMP-1
NM23-H1
níveis de RNA
gliomas
description PURPOSE: To evaluate using transcription analysis the presence and importance of two genes: NM23-H1 and TIMP-1 on control of tumor cell invasion in diffuse astrocytomas (WHO II) and glioblastoma multiforme (WHO IV). METHOD: Northern blot analysis of NM23-H1 and TIMP-1 was performed. Eight diffuse astrocytomas and 19 glioblastomas (WHO IV) were analyzed to determine if TIMP-1 and NM23-H1 were candidates to inhibition of tumor cell invasion quantitated RNA levels. The samples were collected directly from operating room. Total cellular RNA was extracted from frozen tissue samples using guanidinium-isothiocyanate and cesium chloride gradients. Total RNA (10 mg per sample) from tumor tissue were size fractionated through 1% agarose-formaldehyde gel and transferred to nylon filters and then hybridized to 32P-labeled DNA probes and placed for autoradiography. Levels of specific RNAs were determined by computer-assisted laser densitometry. Blot filters were sequentially hybridized to nm23 and TIMP-1 probes in addition to GAPDH, as a control. Statistical analyses were carried out according to t-test for equality of means. RESULTS: NM23-H1 were detected in each sample, however it did not correlate with malignancy and invasiveness. On the other side TIMP-1 gene expression showed a clear correlation between low expression and invasiveness. CONCLUSION: The data suggest that TIMP-1 is an inhibitor of high grade gliomas invasion. NM23-H1 was present in the entire gliomas sample, but it did not vary in diffuse astrocytomas and glioblastomas.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
2015-06-14T13:36:26Z
2015-06-14T13:36:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-282X2006000500014
Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 64, n. 3b, p. 774-780, 2006.
10.1590/S0004-282X2006000500014
S0004-282X2006000500014.pdf
0004-282X
S0004-282X2006000500014
http://repositorio.unifesp.br/handle/11600/3245
url http://dx.doi.org/10.1590/S0004-282X2006000500014
http://repositorio.unifesp.br/handle/11600/3245
identifier_str_mv Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 64, n. 3b, p. 774-780, 2006.
10.1590/S0004-282X2006000500014
S0004-282X2006000500014.pdf
0004-282X
S0004-282X2006000500014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos de Neuro-Psiquiatria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 774-780
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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