Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/7732 http://dx.doi.org/10.1590/0037-8682-1699-2013 |
Resumo: | INTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. |
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Watanabe, Aripuanã Sakurada Aranha [UNIFESP]Carraro, Emerson [UNIFESP]Camargo, Clarice Neves [UNIFESP]Puerari, DianeGuatura, SandraGranato, Celso Francisco Hernandes [UNIFESP]Bellei, Nancy Cristina Junqueira [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade do Centro-Oeste Departamento de farmacologia Clínica2015-06-14T13:45:24Z2015-06-14T13:45:24Z2013-04-09Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013.0037-8682http://repositorio.unifesp.br/handle/11600/7732http://dx.doi.org/10.1590/0037-8682-1699-2013S0037-86822013000200161.pdfS0037-8682201300020016110.1590/0037-8682-1699-2013INTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service.Universidade Federal de São Paulo (UNIFESP) Departamento de Medicina Disciplina de InfectologiaUniversidade do Centro-Oeste Departamento de farmacologia ClínicaUNIFESP, Depto. de Medicina Disciplina de InfectologiaSciELO161-165engSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina TropicalHAdVRespiratory infectionDFANested PCRImmunocompromised patients and immunocompetent patientsHuman adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0037-86822013000200161.pdfapplication/pdf1025854${dspace.ui.url}/bitstream/11600/7732/1/S0037-86822013000200161.pdf5bdf6c4f17a1b93766ae716825d55fb2MD51open accessTEXTS0037-86822013000200161.pdf.txtS0037-86822013000200161.pdf.txtExtracted texttext/plain25501${dspace.ui.url}/bitstream/11600/7732/2/S0037-86822013000200161.pdf.txtdf3204ae4b4d7d518fbca5e8d9217caeMD52open access11600/77322021-10-05 21:50:00.251open accessoai:repositorio.unifesp.br:11600/7732Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T00:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
title |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
spellingShingle |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection Watanabe, Aripuanã Sakurada Aranha [UNIFESP] HAdV Respiratory infection DFA Nested PCR Immunocompromised patients and immunocompetent patients |
title_short |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
title_full |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
title_fullStr |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
title_full_unstemmed |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
title_sort |
Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection |
author |
Watanabe, Aripuanã Sakurada Aranha [UNIFESP] |
author_facet |
Watanabe, Aripuanã Sakurada Aranha [UNIFESP] Carraro, Emerson [UNIFESP] Camargo, Clarice Neves [UNIFESP] Puerari, Diane Guatura, Sandra Granato, Celso Francisco Hernandes [UNIFESP] Bellei, Nancy Cristina Junqueira [UNIFESP] |
author_role |
author |
author2 |
Carraro, Emerson [UNIFESP] Camargo, Clarice Neves [UNIFESP] Puerari, Diane Guatura, Sandra Granato, Celso Francisco Hernandes [UNIFESP] Bellei, Nancy Cristina Junqueira [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade do Centro-Oeste Departamento de farmacologia Clínica |
dc.contributor.author.fl_str_mv |
Watanabe, Aripuanã Sakurada Aranha [UNIFESP] Carraro, Emerson [UNIFESP] Camargo, Clarice Neves [UNIFESP] Puerari, Diane Guatura, Sandra Granato, Celso Francisco Hernandes [UNIFESP] Bellei, Nancy Cristina Junqueira [UNIFESP] |
dc.subject.eng.fl_str_mv |
HAdV Respiratory infection DFA Nested PCR Immunocompromised patients and immunocompetent patients |
topic |
HAdV Respiratory infection DFA Nested PCR Immunocompromised patients and immunocompetent patients |
description |
INTRODUCTION: Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through different methods: direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset. METHODS: Samples (n=643) were collected from different risk groups during from 2001 to 2010: 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program. RESULTS: Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR: 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection. CONCLUSIONS: Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-04-09 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:45:24Z |
dc.date.available.fl_str_mv |
2015-06-14T13:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/7732 http://dx.doi.org/10.1590/0037-8682-1699-2013 |
dc.identifier.issn.none.fl_str_mv |
0037-8682 |
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S0037-86822013000200161.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0037-86822013000200161 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0037-8682-1699-2013 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 2, p. 161-165, 2013. 0037-8682 S0037-86822013000200161.pdf S0037-86822013000200161 10.1590/0037-8682-1699-2013 |
url |
http://repositorio.unifesp.br/handle/11600/7732 http://dx.doi.org/10.1590/0037-8682-1699-2013 |
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eng |
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161-165 |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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