Study of Heparin in Intestinal Ischemia and Reperfusion in Rats: Morphologic and Functional Evaluation

• Autor(a) principal: Ghadie, Majid Mohamad [UNIFESP]
• Data de Publicação: 2012
• Outros Autores: Miranda-Ferreira, Regiane [UNIFESP], Taha, Nabiha Saadi Abrahão [UNIFESP], Maroso, A. S., Moreti, R. J. Z., Andraus, M. P., Zempulski, P., Monteiro, Hugo Pequeno [UNIFESP], Simões, Manuel de Jesus [UNIFESP], Fagundes, Djalma José [UNIFESP], Caricati-Neto, Afonso [UNIFESP], Taha, Murched Omar [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/35307; http://dx.doi.org/10.1016/j.transproceed.2012.07.055

To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R (120 min). After I or I/R, we mounted 2-cm jejunal segment in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl, using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the I + HEP and the I/R + HEP groups, but reduced in the I + SS and the I/R + SS groups. the jejunal enteric nerves were damaged in the I + SS and the I/R + SS, but not in the I + HEP and the I/R + HEP cohorts. These results suggested that HEP attenuated intestinal dysfunction caused by I and I/R.