Papel da fissão mitocondrial na resposta à privação alimentar em caenorhabditis elegans

• Autor(a) principal: Nunes, Danilo Rosa [UNIFESP]
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7676359; https://repositorio.unifesp.br/handle/11600/59621
• topic: Restrição Calórica; Dinâmica Mitocondrial; C. Elegans

Objective: To investigate the impact of the absence of mitochondrial fission on the response to bacterial deprivation in C. elegans. Methods: Wild type worms and drp-1 mutants were grown to adulthood and transferred to NGM plates containing (ad libi- tum condition –AL) or not containing (bacterial deprivation condition -BD) an E. coli lawn. Animals were collected after different time points and mitochondrial parameters such as morphology, oxygen consumption, citrate synthase activity and ATP synthase levels were evaluated. The impact of BD and drp-1 mutation on lifespan and health- span were also assessed. Results: drp-1 mutation did not affect BD-induced exten- sion of life span. BD attenuated the age-related deterioration of body movement in wild type worms but failed to do so in drp-1 mutants, suggesting that mitochondrial fission is necessary for the benefits of BD in the body muscles. Muscle mitochondria seem to be more elongated in drp-1 mutants when compared to wild type animals, and this parameter is not affected by 24 h BD in the studied strains. In spite of the lack of effect of BD on morphology, mitochondrial oxygen consumption rates in live worms was drastically reduced by the nutritional intervention in both strains. This de- crease in respiration is not due to decreased mitochondrial mass, as revealed by the quantification of citrate synthase activity as well as by western immunoblotting for ATP synthase. Conclusion: Decreased mitochondrial fission does not interfere in the effects of BD on life span and mitochondrial activity, but seems to impair the benefits afforded by BD on the age-induced deterioration of mobility.