Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/37898 https://dx.doi.org/10.1111/j.2047-2927.2014.00219.x |
Resumo: | The role of oestrogens in epididymal function is still unclear. Knockout of the oestrogen receptor ESR1 (Esr1-/-) or treatment with the anti-oestrogen Fulvestrant affect epididymal milieu and sperm motility. We investigated the effect of in vivo treatment of rats with Fulvestrant on: (i) expression of genes that may be important for the architecture and function of the epididymal epithelium: prominins 1 and 2, metalloproteinase 7, claudin 7, beta-catenin and cadherin 13, and (ii) levels of oestradiol and testosterone, and expression of oestrogen and androgen receptors, in the initial segment (IS), caput, corpus and cauda epididymis. Fulvestrant (i) reduced gene expression of prominin 1 (variant 1) in the caput, reduced prominin 1 protein content in the caput epididymis and in the efferent ductules, and increased the localization of prominin 1 in microvilli of the caput and corpus; (ii) reduced gene expression of prominin 2 in the corpus and cauda epididymis; (iii) increased the metalloproteinase 7 content in the apical region of principal cells from IS/caput; (iv) reduced in the corpus epididymis, but increased in the efferent ductules, the cadherin 13 mRNA level; (v) reduced testosterone but increased oestradiol levels in the corpus and cauda; (vi) increased the androgen receptor protein content in all regions of the epididymis, and the oestrogen receptor GPER in the corpus and cauda epididymis. in conclusion, treatment with Fulvestrant induced regional-specific changes in hormonal and steroid receptor content, and affected expression of proteins important for epithelial organization and absorption/secretion. the mechanisms of oestrogen action may differ among epididymal regions, which may contribute to determine region-specific sperm functions. |
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Pereira, Marcos Fragoso de Noronha [UNIFESP]Fernandes, Sheilla Alessandra Ferreira [UNIFESP]Nascimento, Aline Rosa [UNIFESP]Siu, Erica Rosanna [UNIFESP]Hess, Rex A.Oliveira, Cleida Aparecida dePorto, Catarina Segreti [UNIFESP]Lazari, Maria de Fatima Magalhaes [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ IllinoisUniversidade Federal de Minas Gerais (UFMG)2016-01-24T14:37:29Z2016-01-24T14:37:29Z2014-07-01Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 4, p. 559-571, 2014.2047-2919https://repositorio.unifesp.br/handle/11600/37898https://dx.doi.org/10.1111/j.2047-2927.2014.00219.x10.1111/j.2047-2927.2014.00219.xWOS:000338038400010The role of oestrogens in epididymal function is still unclear. Knockout of the oestrogen receptor ESR1 (Esr1-/-) or treatment with the anti-oestrogen Fulvestrant affect epididymal milieu and sperm motility. We investigated the effect of in vivo treatment of rats with Fulvestrant on: (i) expression of genes that may be important for the architecture and function of the epididymal epithelium: prominins 1 and 2, metalloproteinase 7, claudin 7, beta-catenin and cadherin 13, and (ii) levels of oestradiol and testosterone, and expression of oestrogen and androgen receptors, in the initial segment (IS), caput, corpus and cauda epididymis. Fulvestrant (i) reduced gene expression of prominin 1 (variant 1) in the caput, reduced prominin 1 protein content in the caput epididymis and in the efferent ductules, and increased the localization of prominin 1 in microvilli of the caput and corpus; (ii) reduced gene expression of prominin 2 in the corpus and cauda epididymis; (iii) increased the metalloproteinase 7 content in the apical region of principal cells from IS/caput; (iv) reduced in the corpus epididymis, but increased in the efferent ductules, the cadherin 13 mRNA level; (v) reduced testosterone but increased oestradiol levels in the corpus and cauda; (vi) increased the androgen receptor protein content in all regions of the epididymis, and the oestrogen receptor GPER in the corpus and cauda epididymis. in conclusion, treatment with Fulvestrant induced regional-specific changes in hormonal and steroid receptor content, and affected expression of proteins important for epithelial organization and absorption/secretion. the mechanisms of oestrogen action may differ among epididymal regions, which may contribute to determine region-specific sperm functions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, BrazilUniv Illinois, Dept Comparat Biosci, Urbana, IL 61801 USAUniv Fed Minas Gerais, Dept Morphol, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, BrazilFAPESP: 2008/56564-1Web of Science559-571engWiley-BlackwellAndrologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessEpididymisOestrogensGene expressionRatAnimal modelsEffects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epitheliuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/378982022-10-10 18:18:13.998metadata only accessoai:repositorio.unifesp.br:11600/37898Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-10-10T21:18:13Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
title |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
spellingShingle |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium Pereira, Marcos Fragoso de Noronha [UNIFESP] Epididymis Oestrogens Gene expression Rat Animal models |
title_short |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
title_full |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
title_fullStr |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
title_full_unstemmed |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
title_sort |
Effects of the oestrogen receptor antagonist Fulvestrant on expression of genes that affect organization of the epididymal epithelium |
author |
Pereira, Marcos Fragoso de Noronha [UNIFESP] |
author_facet |
Pereira, Marcos Fragoso de Noronha [UNIFESP] Fernandes, Sheilla Alessandra Ferreira [UNIFESP] Nascimento, Aline Rosa [UNIFESP] Siu, Erica Rosanna [UNIFESP] Hess, Rex A. Oliveira, Cleida Aparecida de Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
author_role |
author |
author2 |
Fernandes, Sheilla Alessandra Ferreira [UNIFESP] Nascimento, Aline Rosa [UNIFESP] Siu, Erica Rosanna [UNIFESP] Hess, Rex A. Oliveira, Cleida Aparecida de Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Illinois Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Pereira, Marcos Fragoso de Noronha [UNIFESP] Fernandes, Sheilla Alessandra Ferreira [UNIFESP] Nascimento, Aline Rosa [UNIFESP] Siu, Erica Rosanna [UNIFESP] Hess, Rex A. Oliveira, Cleida Aparecida de Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
dc.subject.eng.fl_str_mv |
Epididymis Oestrogens Gene expression Rat Animal models |
topic |
Epididymis Oestrogens Gene expression Rat Animal models |
description |
The role of oestrogens in epididymal function is still unclear. Knockout of the oestrogen receptor ESR1 (Esr1-/-) or treatment with the anti-oestrogen Fulvestrant affect epididymal milieu and sperm motility. We investigated the effect of in vivo treatment of rats with Fulvestrant on: (i) expression of genes that may be important for the architecture and function of the epididymal epithelium: prominins 1 and 2, metalloproteinase 7, claudin 7, beta-catenin and cadherin 13, and (ii) levels of oestradiol and testosterone, and expression of oestrogen and androgen receptors, in the initial segment (IS), caput, corpus and cauda epididymis. Fulvestrant (i) reduced gene expression of prominin 1 (variant 1) in the caput, reduced prominin 1 protein content in the caput epididymis and in the efferent ductules, and increased the localization of prominin 1 in microvilli of the caput and corpus; (ii) reduced gene expression of prominin 2 in the corpus and cauda epididymis; (iii) increased the metalloproteinase 7 content in the apical region of principal cells from IS/caput; (iv) reduced in the corpus epididymis, but increased in the efferent ductules, the cadherin 13 mRNA level; (v) reduced testosterone but increased oestradiol levels in the corpus and cauda; (vi) increased the androgen receptor protein content in all regions of the epididymis, and the oestrogen receptor GPER in the corpus and cauda epididymis. in conclusion, treatment with Fulvestrant induced regional-specific changes in hormonal and steroid receptor content, and affected expression of proteins important for epithelial organization and absorption/secretion. the mechanisms of oestrogen action may differ among epididymal regions, which may contribute to determine region-specific sperm functions. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:29Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 4, p. 559-571, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/37898 https://dx.doi.org/10.1111/j.2047-2927.2014.00219.x |
dc.identifier.issn.none.fl_str_mv |
2047-2919 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.2047-2927.2014.00219.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000338038400010 |
identifier_str_mv |
Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 4, p. 559-571, 2014. 2047-2919 10.1111/j.2047-2927.2014.00219.x WOS:000338038400010 |
url |
https://repositorio.unifesp.br/handle/11600/37898 https://dx.doi.org/10.1111/j.2047-2927.2014.00219.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Andrology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
559-571 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764260658380800 |