PRAME gene expression profile in medulloblastoma

Detalhes bibliográficos
Autor(a) principal: Vulcani-Freitas, Tânia Maria [UNIFESP]
Data de Publicação: 2011
Outros Autores: Saba-silva, Nasjla [UNIFESP], Cappellano, Andréa [UNIFESP], Cavalheiro, Sergio [UNIFESP], Toledo, Silvia Regina Caminada de [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0004-282X2011000100003
http://repositorio.unifesp.br/handle/11600/6321
Resumo: Medulloblastoma is the most common malignant tumors of central nervous system in the childhood. The treatment is severe, harmful and, thus, has a dismal prognosis. As PRAME is present in various cancers, including meduloblastoma, and has limited expression in normal tissues, this antigen can be an ideal vaccine target for tumor immunotherapy. In order to find a potential molecular target, we investigated PRAME expression in medulloblastoma fragments and we compare the results with the clinical features of each patient. Analysis of gene expression was performed by real-time quantitative PCR from 37 tumor samples. The Mann-Whitney test was used to analysis the relationship between gene expression and clinical characteristics. Kaplan-Meier curves were used to evaluate survival. PRAME was overexpressed in 84% samples. But no statistical association was found between clinical features and PRAME overexpression. Despite that PRAME gene could be a strong candidate for immunotherapy since it is highly expressed in medulloblastomas.
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spelling PRAME gene expression profile in medulloblastomaPerfil de expressão do gene PRAME em meduloblastomaPRAMEgene expressionmedulloblastomaimmunotherapyPRAMEexpressão gênicameduloblastomaimunoterapiaMedulloblastoma is the most common malignant tumors of central nervous system in the childhood. The treatment is severe, harmful and, thus, has a dismal prognosis. As PRAME is present in various cancers, including meduloblastoma, and has limited expression in normal tissues, this antigen can be an ideal vaccine target for tumor immunotherapy. In order to find a potential molecular target, we investigated PRAME expression in medulloblastoma fragments and we compare the results with the clinical features of each patient. Analysis of gene expression was performed by real-time quantitative PCR from 37 tumor samples. The Mann-Whitney test was used to analysis the relationship between gene expression and clinical characteristics. Kaplan-Meier curves were used to evaluate survival. PRAME was overexpressed in 84% samples. But no statistical association was found between clinical features and PRAME overexpression. Despite that PRAME gene could be a strong candidate for immunotherapy since it is highly expressed in medulloblastomas.Meduloblastoma é o tumor maligno mais comum em sistema nervoso central na infância. O tratamento é agressivo e o prognóstico é restrito. Como PRAME está presente em vários tumores, incluindo meduloblastoma, e possui baixa expressão em tecidos normais, este antígeno pode ser ideal na imunoterapia. A fim de encontrar um potencial alvo molecular, investigamos a expressão PRAME em fragmentos de meduloblastoma e comparamos os resultados com as características clínicas de cada paciente. Análise da expressão do gene foi realizada por PCR real-time quantitativo em 37 amostras de tumor. O teste de Mann-Whitney foi utilizado para análise da relação entre a expressão do gene e características clínicas e teste de Kaplan-Meier para avaliar a sobrevida. PRAME teve superexpresssão em 84% amostras, mas não houve nenhuma relação estatística entre as características clínicas e superexpressão de PRAME. Apesar disso, o gene PRAME poderia ser um forte candidato para a imunoterapia, pois é altamente expresso em meduloblastomas.Federal University of São Paulo University of São Paulo Pediatric Oncology Institute (GRAACC)Federal University of São Paulo Neurology and Neurosurgery DepartmentFederal University of São Paulo Morphology and Genetic DepartmentUNIFESP, University of São Paulo Pediatric Oncology Institute (GRAACC)UNIFESP, Neurology and Neurosurgery DepartmentUNIFESP, Morphology and Genetic DepartmentSciELOAcademia Brasileira de Neurologia - ABNEUROUniversidade Federal de São Paulo (UNIFESP)Vulcani-Freitas, Tânia Maria [UNIFESP]Saba-silva, Nasjla [UNIFESP]Cappellano, Andréa [UNIFESP]Cavalheiro, Sergio [UNIFESP]Toledo, Silvia Regina Caminada de [UNIFESP]2015-06-14T13:42:56Z2015-06-14T13:42:56Z2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9-12application/pdfhttp://dx.doi.org/10.1590/S0004-282X2011000100003Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 9-12, 2011.10.1590/S0004-282X2011000100003S0004-282X2011000100003.pdf0004-282XS0004-282X2011000100003http://repositorio.unifesp.br/handle/11600/6321WOS:000287809600003engArquivos de Neuro-Psiquiatriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T17:47:12Zoai:repositorio.unifesp.br/:11600/6321Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T17:47:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv PRAME gene expression profile in medulloblastoma
Perfil de expressão do gene PRAME em meduloblastoma
title PRAME gene expression profile in medulloblastoma
spellingShingle PRAME gene expression profile in medulloblastoma
Vulcani-Freitas, Tânia Maria [UNIFESP]
PRAME
gene expression
medulloblastoma
immunotherapy
PRAME
expressão gênica
meduloblastoma
imunoterapia
title_short PRAME gene expression profile in medulloblastoma
title_full PRAME gene expression profile in medulloblastoma
title_fullStr PRAME gene expression profile in medulloblastoma
title_full_unstemmed PRAME gene expression profile in medulloblastoma
title_sort PRAME gene expression profile in medulloblastoma
author Vulcani-Freitas, Tânia Maria [UNIFESP]
author_facet Vulcani-Freitas, Tânia Maria [UNIFESP]
Saba-silva, Nasjla [UNIFESP]
Cappellano, Andréa [UNIFESP]
Cavalheiro, Sergio [UNIFESP]
Toledo, Silvia Regina Caminada de [UNIFESP]
author_role author
author2 Saba-silva, Nasjla [UNIFESP]
Cappellano, Andréa [UNIFESP]
Cavalheiro, Sergio [UNIFESP]
Toledo, Silvia Regina Caminada de [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Vulcani-Freitas, Tânia Maria [UNIFESP]
Saba-silva, Nasjla [UNIFESP]
Cappellano, Andréa [UNIFESP]
Cavalheiro, Sergio [UNIFESP]
Toledo, Silvia Regina Caminada de [UNIFESP]
dc.subject.por.fl_str_mv PRAME
gene expression
medulloblastoma
immunotherapy
PRAME
expressão gênica
meduloblastoma
imunoterapia
topic PRAME
gene expression
medulloblastoma
immunotherapy
PRAME
expressão gênica
meduloblastoma
imunoterapia
description Medulloblastoma is the most common malignant tumors of central nervous system in the childhood. The treatment is severe, harmful and, thus, has a dismal prognosis. As PRAME is present in various cancers, including meduloblastoma, and has limited expression in normal tissues, this antigen can be an ideal vaccine target for tumor immunotherapy. In order to find a potential molecular target, we investigated PRAME expression in medulloblastoma fragments and we compare the results with the clinical features of each patient. Analysis of gene expression was performed by real-time quantitative PCR from 37 tumor samples. The Mann-Whitney test was used to analysis the relationship between gene expression and clinical characteristics. Kaplan-Meier curves were used to evaluate survival. PRAME was overexpressed in 84% samples. But no statistical association was found between clinical features and PRAME overexpression. Despite that PRAME gene could be a strong candidate for immunotherapy since it is highly expressed in medulloblastomas.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2015-06-14T13:42:56Z
2015-06-14T13:42:56Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-282X2011000100003
Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 9-12, 2011.
10.1590/S0004-282X2011000100003
S0004-282X2011000100003.pdf
0004-282X
S0004-282X2011000100003
http://repositorio.unifesp.br/handle/11600/6321
WOS:000287809600003
url http://dx.doi.org/10.1590/S0004-282X2011000100003
http://repositorio.unifesp.br/handle/11600/6321
identifier_str_mv Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 9-12, 2011.
10.1590/S0004-282X2011000100003
S0004-282X2011000100003.pdf
0004-282X
S0004-282X2011000100003
WOS:000287809600003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos de Neuro-Psiquiatria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9-12
application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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