Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia

Detalhes bibliográficos
Autor(a) principal: Paula, Thais Serzedello de [UNIFESP]
Data de Publicação: 2006
Outros Autores: Bertolla, Ricardo Pimenta [UNIFESP], Spaine, Deborah Montagnini [UNIFESP], Cunha, Maria Adelaide [UNIFESP], Schor, Nestor [UNIFESP], Cedenho, Agnaldo Pereira [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.fertnstert.2006.01.047
http://repositorio.unifesp.br/handle/11600/29096
Resumo: Objective: To verify of the increase in sperm apoptotic DNA fragmentation during cryopreservation is greater in oligozoospermic patients than in normozoospermic controls.Design: Controlled prospective study.Setting: patients in an academic research environment.Patient(s): Forty-seven patients with oligozoospermia (concentration < 10 x 10(6) sperm/mL) and 30 normozoospermic men.Intervention(s): Sperm cryopreservation using a standard protocol with a test-yolk buffer, glycerol as the cryoprotectant.Main Outcome Measure(s): Rate of apoptotic sperm DNA fragmentation as assessed by the terminal deoxynucleotidyl-mediated deoxyuridine triphosphate nickend labeling (TUNEL) assay, graded in apoptotic or nonapoptotic, before and after cryopreservation.Result(s): An increase in apoptotic DNA fragmentation was observed in all the groups, regardless of sperm concentration. Normozoospermic men presented a smaller rate of apoptotic DNA fragmentation than oligozoospermic patients, both in pre- and postcryopreservation samples. the increase in DNA fragmentation was similar in both groups.Conclusion(s): Cryopreservation induces apoptotic sperm DNA fragmentation in men, regardless of sperm concentration. Men with oligozoospermia present with higher precryopreservation and postcryopreservation apoptotic sperm DNA fragmentation. the increase in DNA fragmentation is similar in both groups.
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spelling Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermiaoligozoospermiainferfilityspermDNA damageTUNEL assaycryopreservationObjective: To verify of the increase in sperm apoptotic DNA fragmentation during cryopreservation is greater in oligozoospermic patients than in normozoospermic controls.Design: Controlled prospective study.Setting: patients in an academic research environment.Patient(s): Forty-seven patients with oligozoospermia (concentration < 10 x 10(6) sperm/mL) and 30 normozoospermic men.Intervention(s): Sperm cryopreservation using a standard protocol with a test-yolk buffer, glycerol as the cryoprotectant.Main Outcome Measure(s): Rate of apoptotic sperm DNA fragmentation as assessed by the terminal deoxynucleotidyl-mediated deoxyuridine triphosphate nickend labeling (TUNEL) assay, graded in apoptotic or nonapoptotic, before and after cryopreservation.Result(s): An increase in apoptotic DNA fragmentation was observed in all the groups, regardless of sperm concentration. Normozoospermic men presented a smaller rate of apoptotic DNA fragmentation than oligozoospermic patients, both in pre- and postcryopreservation samples. the increase in DNA fragmentation was similar in both groups.Conclusion(s): Cryopreservation induces apoptotic sperm DNA fragmentation in men, regardless of sperm concentration. Men with oligozoospermia present with higher precryopreservation and postcryopreservation apoptotic sperm DNA fragmentation. the increase in DNA fragmentation is similar in both groups.Universidade Federal de São Paulo, Dept Surg, Div Urol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Urol, BR-04038032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04038032 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Paula, Thais Serzedello de [UNIFESP]Bertolla, Ricardo Pimenta [UNIFESP]Spaine, Deborah Montagnini [UNIFESP]Cunha, Maria Adelaide [UNIFESP]Schor, Nestor [UNIFESP]Cedenho, Agnaldo Pereira [UNIFESP]2016-01-24T12:41:24Z2016-01-24T12:41:24Z2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion597-600http://dx.doi.org/10.1016/j.fertnstert.2006.01.047Fertility and Sterility. New York: Elsevier B.V., v. 86, n. 3, p. 597-600, 2006.10.1016/j.fertnstert.2006.01.0470015-0282http://repositorio.unifesp.br/handle/11600/29096WOS:000240998000019engFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-11-04T15:40:32Zoai:repositorio.unifesp.br/:11600/29096Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-11-04T15:40:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
title Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
spellingShingle Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
Paula, Thais Serzedello de [UNIFESP]
oligozoospermia
inferfility
sperm
DNA damage
TUNEL assay
cryopreservation
title_short Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
title_full Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
title_fullStr Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
title_full_unstemmed Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
title_sort Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermia
author Paula, Thais Serzedello de [UNIFESP]
author_facet Paula, Thais Serzedello de [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Cunha, Maria Adelaide [UNIFESP]
Schor, Nestor [UNIFESP]
Cedenho, Agnaldo Pereira [UNIFESP]
author_role author
author2 Bertolla, Ricardo Pimenta [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Cunha, Maria Adelaide [UNIFESP]
Schor, Nestor [UNIFESP]
Cedenho, Agnaldo Pereira [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Paula, Thais Serzedello de [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Cunha, Maria Adelaide [UNIFESP]
Schor, Nestor [UNIFESP]
Cedenho, Agnaldo Pereira [UNIFESP]
dc.subject.por.fl_str_mv oligozoospermia
inferfility
sperm
DNA damage
TUNEL assay
cryopreservation
topic oligozoospermia
inferfility
sperm
DNA damage
TUNEL assay
cryopreservation
description Objective: To verify of the increase in sperm apoptotic DNA fragmentation during cryopreservation is greater in oligozoospermic patients than in normozoospermic controls.Design: Controlled prospective study.Setting: patients in an academic research environment.Patient(s): Forty-seven patients with oligozoospermia (concentration < 10 x 10(6) sperm/mL) and 30 normozoospermic men.Intervention(s): Sperm cryopreservation using a standard protocol with a test-yolk buffer, glycerol as the cryoprotectant.Main Outcome Measure(s): Rate of apoptotic sperm DNA fragmentation as assessed by the terminal deoxynucleotidyl-mediated deoxyuridine triphosphate nickend labeling (TUNEL) assay, graded in apoptotic or nonapoptotic, before and after cryopreservation.Result(s): An increase in apoptotic DNA fragmentation was observed in all the groups, regardless of sperm concentration. Normozoospermic men presented a smaller rate of apoptotic DNA fragmentation than oligozoospermic patients, both in pre- and postcryopreservation samples. the increase in DNA fragmentation was similar in both groups.Conclusion(s): Cryopreservation induces apoptotic sperm DNA fragmentation in men, regardless of sperm concentration. Men with oligozoospermia present with higher precryopreservation and postcryopreservation apoptotic sperm DNA fragmentation. the increase in DNA fragmentation is similar in both groups.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
2016-01-24T12:41:24Z
2016-01-24T12:41:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fertnstert.2006.01.047
Fertility and Sterility. New York: Elsevier B.V., v. 86, n. 3, p. 597-600, 2006.
10.1016/j.fertnstert.2006.01.047
0015-0282
http://repositorio.unifesp.br/handle/11600/29096
WOS:000240998000019
url http://dx.doi.org/10.1016/j.fertnstert.2006.01.047
http://repositorio.unifesp.br/handle/11600/29096
identifier_str_mv Fertility and Sterility. New York: Elsevier B.V., v. 86, n. 3, p. 597-600, 2006.
10.1016/j.fertnstert.2006.01.047
0015-0282
WOS:000240998000019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility and Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 597-600
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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