Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

Detalhes bibliográficos
Autor(a) principal: Nascimento, Ana P. B.
Data de Publicação: 2016
Outros Autores: Ortiz, Mauro F., Martins, Willames Marcos Brasileiro da Silva [UNIFESP], Morais, Guilherme L., Fehlberg, Lorena Cristina Corrêa [UNIFESP], Almeida, Luiz G. P., Ciapina, Luciane P., Gales, Ana Cristina [UNIFESP], Vasconcelos, Ana T. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2016.01946
https://repositorio.unifesp.br/handle/11600/56558
Resumo: Carbapenems represent the mainstay therapy for the treatment of serious P aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-beta-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the bla(spM-1) gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together,these factors could contribute to the marked resistance and persistence of the SPM-1-producing P aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.
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spelling Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitalsdrug resistancecomparative genomicspathogenic bacteriaantimicrobial resistancecarbapenemaseGram-negative bacilliCarbapenems represent the mainstay therapy for the treatment of serious P aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-beta-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the bla(spM-1) gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together,these factors could contribute to the marked resistance and persistence of the SPM-1-producing P aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.Lab Nacl Comp Cient, Lab Bioinformat, Petropolis, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Internal Med, Lab Alerta,Div Infect Dis, Sao Paulo, BrazilLaboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CNPq: 305535/2014-5CNPq: 302768/2011-4CNPq: 312864/2015-9FAPERJ: E-26/202.903/2016Frontiers Media Sa2020-07-31T12:47:04Z2020-07-31T12:47:04Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.3389/fmicb.2016.01946Frontiers In Microbiology. Lausanne, v. 7, p. -, 2016.10.3389/fmicb.2016.01946WOS000389268100001.pdf1664-302Xhttps://repositorio.unifesp.br/handle/11600/56558WOS:000389268100001engFrontiers In MicrobiologyLausanneinfo:eu-repo/semantics/openAccessNascimento, Ana P. B.Ortiz, Mauro F.Martins, Willames Marcos Brasileiro da Silva [UNIFESP]Morais, Guilherme L.Fehlberg, Lorena Cristina Corrêa [UNIFESP]Almeida, Luiz G. P.Ciapina, Luciane P.Gales, Ana Cristina [UNIFESP]Vasconcelos, Ana T. R.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-11T11:59:03Zoai:repositorio.unifesp.br/:11600/56558Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-11T11:59:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
title Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
spellingShingle Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
Nascimento, Ana P. B.
drug resistance
comparative genomics
pathogenic bacteria
antimicrobial resistance
carbapenemase
Gram-negative bacilli
title_short Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
title_full Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
title_fullStr Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
title_full_unstemmed Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
title_sort Intraclonal Genome Stability of the Metallo-beta-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals
author Nascimento, Ana P. B.
author_facet Nascimento, Ana P. B.
Ortiz, Mauro F.
Martins, Willames Marcos Brasileiro da Silva [UNIFESP]
Morais, Guilherme L.
Fehlberg, Lorena Cristina Corrêa [UNIFESP]
Almeida, Luiz G. P.
Ciapina, Luciane P.
Gales, Ana Cristina [UNIFESP]
Vasconcelos, Ana T. R.
author_role author
author2 Ortiz, Mauro F.
Martins, Willames Marcos Brasileiro da Silva [UNIFESP]
Morais, Guilherme L.
Fehlberg, Lorena Cristina Corrêa [UNIFESP]
Almeida, Luiz G. P.
Ciapina, Luciane P.
Gales, Ana Cristina [UNIFESP]
Vasconcelos, Ana T. R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento, Ana P. B.
Ortiz, Mauro F.
Martins, Willames Marcos Brasileiro da Silva [UNIFESP]
Morais, Guilherme L.
Fehlberg, Lorena Cristina Corrêa [UNIFESP]
Almeida, Luiz G. P.
Ciapina, Luciane P.
Gales, Ana Cristina [UNIFESP]
Vasconcelos, Ana T. R.
dc.subject.por.fl_str_mv drug resistance
comparative genomics
pathogenic bacteria
antimicrobial resistance
carbapenemase
Gram-negative bacilli
topic drug resistance
comparative genomics
pathogenic bacteria
antimicrobial resistance
carbapenemase
Gram-negative bacilli
description Carbapenems represent the mainstay therapy for the treatment of serious P aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-beta-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the bla(spM-1) gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together,these factors could contribute to the marked resistance and persistence of the SPM-1-producing P aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-07-31T12:47:04Z
2020-07-31T12:47:04Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2016.01946
Frontiers In Microbiology. Lausanne, v. 7, p. -, 2016.
10.3389/fmicb.2016.01946
WOS000389268100001.pdf
1664-302X
https://repositorio.unifesp.br/handle/11600/56558
WOS:000389268100001
url http://dx.doi.org/10.3389/fmicb.2016.01946
https://repositorio.unifesp.br/handle/11600/56558
identifier_str_mv Frontiers In Microbiology. Lausanne, v. 7, p. -, 2016.
10.3389/fmicb.2016.01946
WOS000389268100001.pdf
1664-302X
WOS:000389268100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers In Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.coverage.none.fl_str_mv Lausanne
dc.publisher.none.fl_str_mv Frontiers Media Sa
publisher.none.fl_str_mv Frontiers Media Sa
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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