High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570 |
Resumo: | Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs. |
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High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazilocular toxoplasmosisToxoplasma gondiigenotypestoxoplasmose ocularToxoplasma gondiigenótiposToxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.Toxoplasmose é a causa mais comum de uveíte infecciosa no Brasil, com maior freqüência no sul do país. Coletamos amostras de diafragma e língua de porcos em pequenos e grandes abatedouros e utilizamos biologia molecular para determinar a taxa de infecção e ''DNA genotyping'' para tipar os parasitas. Dezessete das 50 amostras de diafragma (34%) e 33 das 50 amostras de língua (66%) foram positivas na reação de PCR para T. gondii. A análise restritiva e o sequenciamento do DNA em quatro amostras revelaram que todas apresentam genótipo tipo I no SAG2. No entanto, quando outros loci não ligados foram analisados, estas mesmas amostras se mostraram como tipo III nos marcadores BTUB, SAG3 e GRA6. Uma das amostras (8T) mostrava-se como tipo II no SAG3, indicando um perfil misto. Estas amostras demonstraram não só uma alta taxa de infecção, mas também genótipos incomuns que não foram observados com freqüência em estudos prévios. Nosso trabalho sugere que genótipos incomuns de T. gondii podem ser encontrados no Brasil, até mesmo em porcos domesticados.Universidade Federal de São Paulo (UNIFESP) Departamento de OftalmologiaUniversity of Maryland School of MedicineUniversidade de São Paulo ICB Departamento de ImunologiaClínica SilveiraNIH National Eye InstituteWashington University School of Medicine Department of Molecular BiologyUNIFESP, Depto. de OftalmologiaSciELOAcademia Brasileira de CiênciasUniversidade Federal de São Paulo (UNIFESP)University of Maryland School of MedicineUniversidade de São Paulo (USP)Clínica SilveiraNIH National Eye InstituteWashington University School of Medicine Department of Molecular BiologyBelfort, Rubens Neto [UNIFESP]Nussenblatt, VeroniqueRizzo, Luiz VicenteMuccioli, Cristina [UNIFESP]Silveira, Claudio Alberto Magalhaes [UNIFESP]Nussenblatt, RobertKhan, AssisSibley, L. DavidBelfort, Rubens Junior [UNIFESP]2015-06-14T13:36:45Z2015-06-14T13:36:45Z2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion111-114application/pdfhttp://dx.doi.org/10.1590/S0001-37652007000100013Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007.10.1590/S0001-37652007000100013S0001-37652007000100013.pdf0001-3765S0001-37652007000100013http://repositorio.unifesp.br/handle/11600/3570engAnais da Academia Brasileira de Ciênciasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T13:49:34Zoai:repositorio.unifesp.br/:11600/3570Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T13:49:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| title |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| spellingShingle |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil Belfort, Rubens Neto [UNIFESP] ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos |
| title_short |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| title_full |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| title_fullStr |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| title_full_unstemmed |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| title_sort |
High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil |
| author |
Belfort, Rubens Neto [UNIFESP] |
| author_facet |
Belfort, Rubens Neto [UNIFESP] Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP] |
| author_role |
author |
| author2 |
Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP] |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) University of Maryland School of Medicine Universidade de São Paulo (USP) Clínica Silveira NIH National Eye Institute Washington University School of Medicine Department of Molecular Biology |
| dc.contributor.author.fl_str_mv |
Belfort, Rubens Neto [UNIFESP] Nussenblatt, Veronique Rizzo, Luiz Vicente Muccioli, Cristina [UNIFESP] Silveira, Claudio Alberto Magalhaes [UNIFESP] Nussenblatt, Robert Khan, Assis Sibley, L. David Belfort, Rubens Junior [UNIFESP] |
| dc.subject.por.fl_str_mv |
ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos |
| topic |
ocular toxoplasmosis Toxoplasma gondii genotypes toxoplasmose ocular Toxoplasma gondii genótipos |
| description |
Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-03-01 2015-06-14T13:36:45Z 2015-06-14T13:36:45Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
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http://dx.doi.org/10.1590/S0001-37652007000100013 Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007. 10.1590/S0001-37652007000100013 S0001-37652007000100013.pdf 0001-3765 S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570 |
| url |
http://dx.doi.org/10.1590/S0001-37652007000100013 http://repositorio.unifesp.br/handle/11600/3570 |
| identifier_str_mv |
Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 79, n. 1, p. 111-114, 2007. 10.1590/S0001-37652007000100013 S0001-37652007000100013.pdf 0001-3765 S0001-37652007000100013 |
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eng |
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eng |
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Anais da Academia Brasileira de Ciências |
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info:eu-repo/semantics/openAccess |
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openAccess |
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111-114 application/pdf |
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Academia Brasileira de Ciências |
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Academia Brasileira de Ciências |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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