Ações da eletroacupuntura (eea) aplicada ao ponto pc6 (nei guan) sobre a resposta central de estresse em ratos

• Autor(a) principal: Bassetto, Ricardo Morad [UNIFESP]
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=2483238; https://repositorio.unifesp.br/handle/11600/47876
• topic: Stress; Anxiety; Electroacupuncture; Neiguan (pc6); Paraventricular nucleus of the hypothalamus; Dorsal medial hypothalamus; Prefrontal cortex; Estresse; Ansiedade; Eletroacupuntura; Neiguan (pc6); Núcleo paraventricular do hipotálamo; Hipotálamo dorso medial; Córtex pré-frontal

The stress response plays an important biological role preparing the body to respond to physical and mental stressors. However, it might trigger and / or worsen nosological conditions for which there is individual predisposition, when in duration and intensity that exceed the capacity of psychobiological functional adaptation. Among psychiatric disorders, anxiety disorders maintain pathophysiological nexus with intense acute stress or chronic stress and, among other systems, the cardiovascular system is particularly susceptible to adaptive changes triggered by both the stress response and the anxious state. The objective of this study was to investigate the effectiveness of electroacupuncture (EEA) applied to the Neiguan point (PC6), classically described as an anxiolytic and cardiocirculatory regulator, and to points classically not described and without recognized therapeutic indications, located in the tail (Tail), in the modulation of the stress response, anxiety and superior control of cardiovascular functions. For this, thirty-two male Wistar rats, with about 8 and a half weeks of age and weighing between 240 and 300 g, were distributed into four groups: 1. eight rats not subjected to any procedure (control group); 2. eight rats subjected to immobilization (IMO) for 60 min on three consecutive days (IMO group); 3. eight rats subjected to IMO associated with the EEA in PC6 (PC6 group); 4. eight rats subjected to IMO associated with EEA in the tail (TAIL group). Behavioral parameters associated with anxiety, through the elevated plus maze test (EPM), and the neuronal activation levels through the immunoreactivity to Fos protein in areas associated with the stress response (paraventricular nucleus of the hypothalamus-PVH), anxiety (medial amygdalameAMI, central amygdala-ceAMI, hippocampal formation - dentate gyrus-GD, CA1,CA2, CA3 - and prefrontal cortex-CPF), and superior central control of the cardiovascular functions (lateral hypothalamic area-HIPOT LAT and dorsal medial hypothalamus-DMH) have been analyzed. The statistically significant data, p <0.05, generated in the present study show that, in rats subjected to immobilization, EEA, whether applied to PC6 or the tail, reduced the behavioral parameter indicative of anxiety, "grooming", to numbers equivalent to the control group, but there were no significant differences in other behavioral parameters associated with anxiety. The immunoreactivity to Fos protein showed a significant attenuation of neuronal activation which was triggered by IMO in rats that were treated with EEA, in all 8 studied encephalic areas, and this attenuation was significantly larger in the PVH, CPF and DMH of rats given EEA in the PC6 point when compared with rats that received EEA in the tail. These experimental data suggest a therapeutic potential for the EEA in the modulation of stress response, which can be related to the development of anxiety disorders and pathologies of the cardiovascular system, with relative specificity when applied to the PC6 point, for its significantly differentiated action in areas directly related to stress response (PVH), anxiety (CPF) and superior control of cardiovascular functions facing stress (DMH).