Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0102-86502011000200006 http://repositorio.unifesp.br/handle/11600/6396 |
Resumo: | PURPOSE: To evaluate the effects of mechanical ventilation (MV) of high-oxygen concentration in pulmonary dysfunction in adult and elderly rats. METHODS: Twenty-eight adult (A) and elderly (E), male rats were ventilated for 1 hour (G-AV1 and G-EV1) or for 3 hours (G-AV3 and G-EV3). A and E groups received a tidal volume of 7 mL/kg, a positive end-expiratory pressure of 5 cm H2O, respiratory rate of 70 cycles per minute, and an inspiratory fraction of oxygen of 1. We evaluated total protein content and malondialdehyde in bronchoalveolar lavages (BAL) and performed lung histomorphometrical analyses. RESULTS: In G-EV1 animals, total protein in BAL was higher (33.0±1.9 µg/mL) compared with G-AV1 (23.0±2.0 µg/mL). Upon 180 minutes of MV, malondialdehyde levels increased in elderly (G-EV3) compared with adult (G-AV3) groups. Malondialdehyde and total proteins in BAL after 3 hours of MV were higher in elderly group than in adults. In G-EV3 group we observed alveolar septa dilatation and significative increase in neutrofiles number in relation to adult group at 60 and 180 minutes on MV. CONCLUSION: A higher fraction of inspired oxygen in short courses of mechanical ventilation ameliorates the parameters studied in elderly lungs. |
| id |
UFSP_89702357f02b5cb76caa96c97e91be17 |
|---|---|
| oai_identifier_str |
oai:repositorio.unifesp.br/:11600/6396 |
| network_acronym_str |
UFSP |
| network_name_str |
Repositório Institucional da UNIFESP |
| repository_id_str |
3465 |
| spelling |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungsVentilação mecânica de curta duração com altos níveis de O2 em pulmões de ratos idososAgingLung injuryVentilator-Induced Lung InjuryNeutrophil InfiltrationOxidative StressRatsEnvelhecimentoLesão PulmonarLesão Pulmonar Induzida por Ventilação MecânicaInfiltração de NeutrófilosEstresse OxidativoRatosPURPOSE: To evaluate the effects of mechanical ventilation (MV) of high-oxygen concentration in pulmonary dysfunction in adult and elderly rats. METHODS: Twenty-eight adult (A) and elderly (E), male rats were ventilated for 1 hour (G-AV1 and G-EV1) or for 3 hours (G-AV3 and G-EV3). A and E groups received a tidal volume of 7 mL/kg, a positive end-expiratory pressure of 5 cm H2O, respiratory rate of 70 cycles per minute, and an inspiratory fraction of oxygen of 1. We evaluated total protein content and malondialdehyde in bronchoalveolar lavages (BAL) and performed lung histomorphometrical analyses. RESULTS: In G-EV1 animals, total protein in BAL was higher (33.0±1.9 µg/mL) compared with G-AV1 (23.0±2.0 µg/mL). Upon 180 minutes of MV, malondialdehyde levels increased in elderly (G-EV3) compared with adult (G-AV3) groups. Malondialdehyde and total proteins in BAL after 3 hours of MV were higher in elderly group than in adults. In G-EV3 group we observed alveolar septa dilatation and significative increase in neutrofiles number in relation to adult group at 60 and 180 minutes on MV. CONCLUSION: A higher fraction of inspired oxygen in short courses of mechanical ventilation ameliorates the parameters studied in elderly lungs.OBJETIVO: Avaliar os efeitos da ventilação mecânica com alta concentração de oxigênio em animais adultos e idosos. MÉTODOS: Vinte e oito ratos machos (adultos e idosos; n=7 por grupo) foram divididos em ventilados por 1 hora (G-AV1 e G-EV1) e ventilados por 3 horas (G-AV3 e G-EV3). Os grupos receberam volume corrente de 7 mL/kg, pressão positive ao final da expiração de 5 cmH2O, frequência respiratória de 70 ciclos por minuto e fração inspirada de oxigênio de 100%. Ao final do experimento avaliamos no lavado brônquio-alveolar as proteínas totais, malondialdeído, celularidade e a histomorfometria do parênquima pulmonar. RESULTADOS: Em animais idosos ventilados por 1 hora (G-EV1) as proteínas totais no lavado brônquio-alveolar aumentaram (33.0±1.9 µg/mL) quando comparados com os adultos (G-AV1; 23.0±2.0 µg/mL). Após 180 minutos de ventilação mecânica os níveis de malondialdeído e as proteínas totais estavam elevadas nos animais idosos (G-EV3) quando comparadas com os adultos (G-AV3; p<0.001). No grupo de animais idosos (G-EV3) observamos ainda dilatação dos septos alveolares e aumento significativo no número de neutrófilos em relação aos adultos ventilados, tanto aos 60 quanto aos 180 minutos de ventilação mecânica (p<0.001). CONCLUSÃO: A ventilação mecânica com alta fração inspirada de oxigênio por um curto período de tempo favoreceu os parâmetros pulmonares estudados nos animais idosos.Universidade Federal de São Paulo (UNIFESP) Department of Preventive MedicineUniversidade Federal de São Paulo (UNIFESP) Department of Biological ScienceUNIFESP Division of Anesthesiology, Pain and Intensive TherapyPaulista State University Department of Production Support and Animal HealthUniversidade Federal de São Paulo (UNIFESP) Department of Morphology and Genetics Histology DivisionUNIFESP Division of Geriatrics and GerontologyUNIFESP, Department of Preventive MedicineUNIFESP, Department of Biological ScienceUNIFESP, Division of Anesthesiology, Pain and Intensive TherapyUNIFESP, Department of Morphology and Genetics Histology DivisionUNIFESP, Division of Geriatrics and GerontologySciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (UNESP)Cavassani, Sâmia Santos [UNIFESP]Junqueira, Virginia Berlanga Campos [UNIFESP]Moraes, Juliana Britto [UNIFESP]Luzo, Karen Kristina [UNIFESP]Silva, Claudeny Matos Assis [UNIFESP]Barros, Maciel [UNIFESP]Marinho, MárciaSimões, Ricardo Santos [UNIFESP]Oliveira-Júnior, Itamar Souza [UNIFESP]2015-06-14T13:43:00Z2015-06-14T13:43:00Z2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion107-113application/pdfhttp://dx.doi.org/10.1590/S0102-86502011000200006Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 2, p. 107-113, 2011.10.1590/S0102-86502011000200006S0102-86502011000200006.pdf0102-8650S0102-86502011000200006http://repositorio.unifesp.br/handle/11600/6396WOS:000288866800006engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-27T08:34:41Zoai:repositorio.unifesp.br/:11600/6396Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-27T08:34:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs Ventilação mecânica de curta duração com altos níveis de O2 em pulmões de ratos idosos |
| title |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| spellingShingle |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs Cavassani, Sâmia Santos [UNIFESP] Aging Lung injury Ventilator-Induced Lung Injury Neutrophil Infiltration Oxidative Stress Rats Envelhecimento Lesão Pulmonar Lesão Pulmonar Induzida por Ventilação Mecânica Infiltração de Neutrófilos Estresse Oxidativo Ratos |
| title_short |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| title_full |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| title_fullStr |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| title_full_unstemmed |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| title_sort |
Short courses of mechanical ventilation with high-O2 levels in elderly rat lungs |
| author |
Cavassani, Sâmia Santos [UNIFESP] |
| author_facet |
Cavassani, Sâmia Santos [UNIFESP] Junqueira, Virginia Berlanga Campos [UNIFESP] Moraes, Juliana Britto [UNIFESP] Luzo, Karen Kristina [UNIFESP] Silva, Claudeny Matos Assis [UNIFESP] Barros, Maciel [UNIFESP] Marinho, Márcia Simões, Ricardo Santos [UNIFESP] Oliveira-Júnior, Itamar Souza [UNIFESP] |
| author_role |
author |
| author2 |
Junqueira, Virginia Berlanga Campos [UNIFESP] Moraes, Juliana Britto [UNIFESP] Luzo, Karen Kristina [UNIFESP] Silva, Claudeny Matos Assis [UNIFESP] Barros, Maciel [UNIFESP] Marinho, Márcia Simões, Ricardo Santos [UNIFESP] Oliveira-Júnior, Itamar Souza [UNIFESP] |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (UNESP) |
| dc.contributor.author.fl_str_mv |
Cavassani, Sâmia Santos [UNIFESP] Junqueira, Virginia Berlanga Campos [UNIFESP] Moraes, Juliana Britto [UNIFESP] Luzo, Karen Kristina [UNIFESP] Silva, Claudeny Matos Assis [UNIFESP] Barros, Maciel [UNIFESP] Marinho, Márcia Simões, Ricardo Santos [UNIFESP] Oliveira-Júnior, Itamar Souza [UNIFESP] |
| dc.subject.por.fl_str_mv |
Aging Lung injury Ventilator-Induced Lung Injury Neutrophil Infiltration Oxidative Stress Rats Envelhecimento Lesão Pulmonar Lesão Pulmonar Induzida por Ventilação Mecânica Infiltração de Neutrófilos Estresse Oxidativo Ratos |
| topic |
Aging Lung injury Ventilator-Induced Lung Injury Neutrophil Infiltration Oxidative Stress Rats Envelhecimento Lesão Pulmonar Lesão Pulmonar Induzida por Ventilação Mecânica Infiltração de Neutrófilos Estresse Oxidativo Ratos |
| description |
PURPOSE: To evaluate the effects of mechanical ventilation (MV) of high-oxygen concentration in pulmonary dysfunction in adult and elderly rats. METHODS: Twenty-eight adult (A) and elderly (E), male rats were ventilated for 1 hour (G-AV1 and G-EV1) or for 3 hours (G-AV3 and G-EV3). A and E groups received a tidal volume of 7 mL/kg, a positive end-expiratory pressure of 5 cm H2O, respiratory rate of 70 cycles per minute, and an inspiratory fraction of oxygen of 1. We evaluated total protein content and malondialdehyde in bronchoalveolar lavages (BAL) and performed lung histomorphometrical analyses. RESULTS: In G-EV1 animals, total protein in BAL was higher (33.0±1.9 µg/mL) compared with G-AV1 (23.0±2.0 µg/mL). Upon 180 minutes of MV, malondialdehyde levels increased in elderly (G-EV3) compared with adult (G-AV3) groups. Malondialdehyde and total proteins in BAL after 3 hours of MV were higher in elderly group than in adults. In G-EV3 group we observed alveolar septa dilatation and significative increase in neutrofiles number in relation to adult group at 60 and 180 minutes on MV. CONCLUSION: A higher fraction of inspired oxygen in short courses of mechanical ventilation ameliorates the parameters studied in elderly lungs. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-04-01 2015-06-14T13:43:00Z 2015-06-14T13:43:00Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502011000200006 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 2, p. 107-113, 2011. 10.1590/S0102-86502011000200006 S0102-86502011000200006.pdf 0102-8650 S0102-86502011000200006 http://repositorio.unifesp.br/handle/11600/6396 WOS:000288866800006 |
| url |
http://dx.doi.org/10.1590/S0102-86502011000200006 http://repositorio.unifesp.br/handle/11600/6396 |
| identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 2, p. 107-113, 2011. 10.1590/S0102-86502011000200006 S0102-86502011000200006.pdf 0102-8650 S0102-86502011000200006 WOS:000288866800006 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
107-113 application/pdf |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268347397701632 |