Características clínicas e frequência de polimorfismos em TLR4 em pacientes brasileiros com espondilite anquilosante

• Autor(a) principal: Machado, Natalia Pereira [UNIFESP]
• Data de Publicação: 2016
• Outros Autores: Nogueira, Eliana [UNIFESP], Oseki, Karen [UNIFESP], Cruz Ebbing, Pamela Carolina [UNIFESP], Taemi Origassa, Clarice Silvia [UNIFESP], Mohovic, Tatiane [UNIFESP], Saraiva Camara, Niels Olsen [UNIFESP], Pinheiro, Marcelo de Medeiros [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng; por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1016/j.rbr.2016.05.004; http://repositorio.unifesp.br/handle/11600/51055
• topic: Ankylosing spondylitis; TLR-4 polymorphisms; HLA-B27; Espondilite anquilosante; Polimorfismos em TLR-4; HLA-B27

Objectives: Innate immunity is involved in the physiopathology of ankylosing spondylitis (AS), with the participation of Gram-negative bacteria, modulation of human leukocyte antigen (HLA) B27 and the involvement of pattern recognition receptors, such as Toll-like receptors (TLRs). The aim of this study was to investigate the clinical characteristics and frequency of TLR4 polymorphisms (Asp299Gly and Thr 399Ile) in a cohort of Brazilian patients with AS. Methods: A cross-sectional study was carried out involving 200 patients with a diagnosis of AS and a healthy control group of 200 individuals. Disease activity, severity and functional capacity were measured. The study of TLR4 polymorphisms was performed using the restriction fragment length polymorphism method. HLA-B27 was analyzed by conventional polymerase chain reaction. The IBM SPSS Statistics 20 program was used for the statistical analysis, with p-values less than 0.05 considered significant. Results: Mean age and disease duration were 43.1 +/- 12.7 and 16.6 +/- 9.2 years, respectively. The sample was predominantly male (71%) and non-Caucasian (52%). A total of 66% of the group of patients were positive for HLA-B27. The sample of patients was characterized by moderate functional impairment and a high degree of disease activity. No significant association was found between the two TLR4 polymorphisms and susceptibility to AS. Conclusions: TLR4 polymorphisms 399 and 299 were not more frequent in patients with AS in comparison to the health controls and none of the clinical variables were associated with these polymorphisms.(C) 2016 Published by Elsevier Editora Ltda.