Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/s12866-017-1063-x https://repositorio.unifesp.br/handle/11600/53453 |
Resumo: | Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants'Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant. |
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Moreira, Leandro M.Soares, Marcia R.Facincani, Agda P.Ferreira, Cristiano B.Ferreira, Rafael M.Ferro, Maria I. T.Gozzo, Fabio C.Felestrino, Erica B.Assis, Renata A. B.Garcia, Camila Carriao M.Setubal, Joao C. [UNIFESP]Ferro, Jesus A.de Oliveira, Julio C. F. [UNIFESP]2020-06-26T16:30:16Z2020-06-26T16:30:16Z2017http://dx.doi.org/10.1186/s12866-017-1063-xBmc Microbiology. London, v. 17, p. -, 2017.1471-2180https://repositorio.unifesp.br/handle/11600/53453WOS000405421700003.pdf10.1186/s12866-017-1063-xWOS:000405421700003Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants'Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Fundacao de Amparo a Pesquisa do Estado de Sao PauloFundacao de Amparo a Pesquisa do Estado de Minas GeraisBIGA grant-CAPESUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol DECBI, Ouro Preto, MG, BrazilUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, BrazilUniv Fed Rio de Janeiro, Inst Quim, Dept Bioquim DBq, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, UNESP, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, BrazilUniv Estadual Campinas, UNICAMP, Inst Quim, Campinas, SP, BrazilUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, BrazilVirginia Tech, Biocomplex Inst, Blacksburg, VA USAUniv Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, BrazilCAPESFAPESP: 04/02006-7Fundacao de Amparo a Pesquisa do Estado de Minas Gerais: CBB-APQ-04425-10BIGA grant-CAPES: 3385/2013Web of Science-engBiomed Central LtdBmc MicrobiologyXanthomonasadaptation mechanismsBiofilmIron uptake and metabolismPlants'Oxidative burstLPS modulationProteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon17info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000405421700003.pdfapplication/pdf2430075${dspace.ui.url}/bitstream/11600/53453/1/WOS000405421700003.pdfed62c64453d6cd5e68fa601681c87abcMD51open accessTEXTWOS000405421700003.pdf.txtWOS000405421700003.pdf.txtExtracted texttext/plain103012${dspace.ui.url}/bitstream/11600/53453/8/WOS000405421700003.pdf.txtcb76b2de9ca4dbf65594cd0c42136202MD58open accessTHUMBNAILWOS000405421700003.pdf.jpgWOS000405421700003.pdf.jpgIM Thumbnailimage/jpeg6374${dspace.ui.url}/bitstream/11600/53453/10/WOS000405421700003.pdf.jpgad77e17da196c3b7934bcabf488c21e9MD510open access11600/534532023-06-05 19:21:24.261open accessoai:repositorio.unifesp.br:11600/53453Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:21:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
title |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
spellingShingle |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection Moreira, Leandro M. Xanthomonas adaptation mechanisms Biofilm Iron uptake and metabolism Plants'Oxidative burst LPS modulation |
title_short |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
title_full |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
title_fullStr |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
title_full_unstemmed |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
title_sort |
Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp citri during Citrus sinensis infection |
author |
Moreira, Leandro M. |
author_facet |
Moreira, Leandro M. Soares, Marcia R. Facincani, Agda P. Ferreira, Cristiano B. Ferreira, Rafael M. Ferro, Maria I. T. Gozzo, Fabio C. Felestrino, Erica B. Assis, Renata A. B. Garcia, Camila Carriao M. Setubal, Joao C. [UNIFESP] Ferro, Jesus A. de Oliveira, Julio C. F. [UNIFESP] |
author_role |
author |
author2 |
Soares, Marcia R. Facincani, Agda P. Ferreira, Cristiano B. Ferreira, Rafael M. Ferro, Maria I. T. Gozzo, Fabio C. Felestrino, Erica B. Assis, Renata A. B. Garcia, Camila Carriao M. Setubal, Joao C. [UNIFESP] Ferro, Jesus A. de Oliveira, Julio C. F. [UNIFESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Moreira, Leandro M. Soares, Marcia R. Facincani, Agda P. Ferreira, Cristiano B. Ferreira, Rafael M. Ferro, Maria I. T. Gozzo, Fabio C. Felestrino, Erica B. Assis, Renata A. B. Garcia, Camila Carriao M. Setubal, Joao C. [UNIFESP] Ferro, Jesus A. de Oliveira, Julio C. F. [UNIFESP] |
dc.subject.eng.fl_str_mv |
Xanthomonas adaptation mechanisms Biofilm Iron uptake and metabolism Plants'Oxidative burst LPS modulation |
topic |
Xanthomonas adaptation mechanisms Biofilm Iron uptake and metabolism Plants'Oxidative burst LPS modulation |
description |
Background: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection. Results: For that, a 2D-based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants'Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants. Conclusions: This is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:16Z |
dc.date.available.fl_str_mv |
2020-06-26T16:30:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.].fl_str_mv |
http://dx.doi.org/10.1186/s12866-017-1063-x |
dc.identifier.citation.fl_str_mv |
Bmc Microbiology. London, v. 17, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53453 |
dc.identifier.issn.none.fl_str_mv |
1471-2180 |
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WOS000405421700003.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/s12866-017-1063-x |
dc.identifier.wos.none.fl_str_mv |
WOS:000405421700003 |
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http://dx.doi.org/10.1186/s12866-017-1063-x https://repositorio.unifesp.br/handle/11600/53453 |
identifier_str_mv |
Bmc Microbiology. London, v. 17, p. -, 2017. 1471-2180 WOS000405421700003.pdf 10.1186/s12866-017-1063-x WOS:000405421700003 |
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eng |
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eng |
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Bmc Microbiology |
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- |
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London |
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Biomed Central Ltd |
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Biomed Central Ltd |
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