Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0001-37652009000300009 http://repositorio.unifesp.br/handle/11600/5259 |
Resumo: | Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host. |
id |
UFSP_8bc4f2696ddab17bcb28ebc917175b1a |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/5259 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cellsneural precursor cellsneurospherefibroblast growth factor 2epidermal growth factordifferentiationcélulas precursoras neuraisneuroesferafator de crescimento de fibroblasto 2fator de crescimento epidérmicodiferenciaçãoCell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.As terapias celulares para doenças neurológicas têm avançado e células precursoras neurais (NPC) surgem como candidatas ideais para o transplante de células neurais em muitas doenças. No entanto, trabalhos adicionais devem ser feitos para determinar o ambiente de cultivo ideal para a expansão in vitro das NPC, sem alterar sua plasticidade, e os mecanismos de sinalização celular do fator de crescimento epidérmico (EGF) e fator de crescimento de fibroblasto 2 (FGF-2) no crescimento, sobrevivência e diferenciação da neuroesfera. Nesse trabalho avaliamosNPCcultivadas na presença e na ausência de FGF-2 e EGF e mostramos que esses fatores de crescimento são responsáveis pela proliferação das NPC. Também foi demonstrado que a produção endógena de fatores de crescimento alterna de FGF-2 a fator de crescimento de insulina 1 (IGF-1) e fator de crescimento derivado de plaquetas b (PDGFb) após remoção de EGF e FGF-2. NPC de camundongo cultivadas em suspensão mostraram padrões de localização neuronal distintos (centro versus borda) tanto no grupo controle como no grupo sem EGF e FGF-2. Juntos, esses resultados mostram que a remoção de EGF e FGF-2 exerce importante ação na diferenciação de NPC e possivelmente contribui para melhor compreensão dos mecanismos envolvidos na diferenciação. Nossos achados sugerem que, privando as NPC de fatores de crescimento antes do transplante, talvez aumente as chances de que as células efetivamente se integrem ao hospedeiro.Universidade Federal de São Paulo (UNIFESP) Departamento de FisiologiaUniversidade Federal de São Paulo (UNIFESP) Departamento de BiofísicaUNIFESP, Depto. de FisiologiaUNIFESP, Depto. de BiofísicaSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Academia Brasileira de CiênciasUniversidade Federal de São Paulo (UNIFESP)Schwindt, Telma Tiemi [UNIFESP]Motta, Fabiana Louise Teixeira [UNIFESP]Barnabé, Gabriela Filoso [UNIFESP]Cristina G., Massant [UNIFESP]Guimarães, Alessander de Oliveira [UNIFESP]Calcagnotto, Maria Elisa [UNIFESP]Pesquero, João Bosco [UNIFESP]Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]2015-06-14T13:41:10Z2015-06-14T13:41:10Z2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion443-452application/pdfhttp://dx.doi.org/10.1590/S0001-37652009000300009Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 81, n. 3, p. 443-452, 2009.10.1590/S0001-37652009000300009S0001-37652009000300009.pdf0001-3765S0001-37652009000300009http://repositorio.unifesp.br/handle/11600/5259WOS:000269462300009engAnais da Academia Brasileira de Ciênciasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T09:32:57Zoai:repositorio.unifesp.br/:11600/5259Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T09:32:57Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
title |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
spellingShingle |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells Schwindt, Telma Tiemi [UNIFESP] neural precursor cells neurosphere fibroblast growth factor 2 epidermal growth factor differentiation células precursoras neurais neuroesfera fator de crescimento de fibroblasto 2 fator de crescimento epidérmico diferenciação |
title_short |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
title_full |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
title_fullStr |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
title_full_unstemmed |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
title_sort |
Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells |
author |
Schwindt, Telma Tiemi [UNIFESP] |
author_facet |
Schwindt, Telma Tiemi [UNIFESP] Motta, Fabiana Louise Teixeira [UNIFESP] Barnabé, Gabriela Filoso [UNIFESP] Cristina G., Massant [UNIFESP] Guimarães, Alessander de Oliveira [UNIFESP] Calcagnotto, Maria Elisa [UNIFESP] Pesquero, João Bosco [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] |
author_role |
author |
author2 |
Motta, Fabiana Louise Teixeira [UNIFESP] Barnabé, Gabriela Filoso [UNIFESP] Cristina G., Massant [UNIFESP] Guimarães, Alessander de Oliveira [UNIFESP] Calcagnotto, Maria Elisa [UNIFESP] Pesquero, João Bosco [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Schwindt, Telma Tiemi [UNIFESP] Motta, Fabiana Louise Teixeira [UNIFESP] Barnabé, Gabriela Filoso [UNIFESP] Cristina G., Massant [UNIFESP] Guimarães, Alessander de Oliveira [UNIFESP] Calcagnotto, Maria Elisa [UNIFESP] Pesquero, João Bosco [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] |
dc.subject.por.fl_str_mv |
neural precursor cells neurosphere fibroblast growth factor 2 epidermal growth factor differentiation células precursoras neurais neuroesfera fator de crescimento de fibroblasto 2 fator de crescimento epidérmico diferenciação |
topic |
neural precursor cells neurosphere fibroblast growth factor 2 epidermal growth factor differentiation células precursoras neurais neuroesfera fator de crescimento de fibroblasto 2 fator de crescimento epidérmico diferenciação |
description |
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 2015-06-14T13:41:10Z 2015-06-14T13:41:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0001-37652009000300009 Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 81, n. 3, p. 443-452, 2009. 10.1590/S0001-37652009000300009 S0001-37652009000300009.pdf 0001-3765 S0001-37652009000300009 http://repositorio.unifesp.br/handle/11600/5259 WOS:000269462300009 |
url |
http://dx.doi.org/10.1590/S0001-37652009000300009 http://repositorio.unifesp.br/handle/11600/5259 |
identifier_str_mv |
Anais da Academia Brasileira de Ciências. Academia Brasileira de Ciências, v. 81, n. 3, p. 443-452, 2009. 10.1590/S0001-37652009000300009 S0001-37652009000300009.pdf 0001-3765 S0001-37652009000300009 WOS:000269462300009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais da Academia Brasileira de Ciências |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
443-452 application/pdf |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268368532799488 |