Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways

Detalhes bibliográficos
Autor(a) principal: Dorsa, Karina Kores [UNIFESP]
Data de Publicação: 2010
Outros Autores: Santos, Michelle Venâncio Dos [UNIFESP], Dias-da-Silva, Magnus Régios [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0004-27302010000400007
http://repositorio.unifesp.br/handle/11600/5754
Resumo: OBJECTIVE: We sought to identify glycolysis, glycogenolysis, lipolysis, Krebs cycle, respiratory chain, and oxidative phosphorylation enzymes simultaneously regulated by T3 and cAMP. MATERIALS AND METHODS: We performed in silico analysis of 56 promoters to search for cis-cAMP (CREB) and cis-thyroid (TRE) response elements, considering UCP1, SERCA2 and glyceraldehyde 3-phosphate dehydrogenase as reference. Only regulatory regions with prior in vitro validation were selected. RESULTS: 29/56 enzymes presented potential TREs in their regulatory sequence, and some scored over 0.80 (better predictive value 1): citrate synthase, phosphoglucose isomerase, succinate dehydrogenases A/C, UCP3, UCP2, UCP4, UCP5, phosphoglycerate mutase, glyceraldehyde 3-P dehydrogenase, glucokinase, malate dehydrogenase, acyl-CoA transferase (thiolase), cytochrome a3, and lactate dehydrogenase. Moreover, some enzymes have not yet been described in the literature as genomically regulated by T3. CONCLUSION: Our results point to other enzymes which may possibly be regulated by T3 and CREB, and speculate their joint roles in contributing to the optimal thermogenic acclimation.
id UFSP_8dabcc3da3dc1245e306d55ccc71cbf8
oai_identifier_str oai:repositorio.unifesp.br/:11600/5754
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathwaysAmpliando a participação dos genes responsivos a T3 e cAMP na regulação da termogênese gerada pelas vias de oxidação biológicaThermogenesisT3RCREBgenomic regulationfuel oxidationTermogêneseT3RCREBregulação gênicaoxidação biológicaOBJECTIVE: We sought to identify glycolysis, glycogenolysis, lipolysis, Krebs cycle, respiratory chain, and oxidative phosphorylation enzymes simultaneously regulated by T3 and cAMP. MATERIALS AND METHODS: We performed in silico analysis of 56 promoters to search for cis-cAMP (CREB) and cis-thyroid (TRE) response elements, considering UCP1, SERCA2 and glyceraldehyde 3-phosphate dehydrogenase as reference. Only regulatory regions with prior in vitro validation were selected. RESULTS: 29/56 enzymes presented potential TREs in their regulatory sequence, and some scored over 0.80 (better predictive value 1): citrate synthase, phosphoglucose isomerase, succinate dehydrogenases A/C, UCP3, UCP2, UCP4, UCP5, phosphoglycerate mutase, glyceraldehyde 3-P dehydrogenase, glucokinase, malate dehydrogenase, acyl-CoA transferase (thiolase), cytochrome a3, and lactate dehydrogenase. Moreover, some enzymes have not yet been described in the literature as genomically regulated by T3. CONCLUSION: Our results point to other enzymes which may possibly be regulated by T3 and CREB, and speculate their joint roles in contributing to the optimal thermogenic acclimation.OBJETIVO: Identificar enzimas das vias da glicólise, glicogenólise, lipólise, ciclo de Krebs, cadeia respiratória e fosforilação oxidativa possivelmente reguladas por T3 e cAMP. MATERIAIS E MÉTODOS: Analisamos 56 genes metabólicos in silico mediante a identificação dos elementos cis de regulação gênica responsivos ao T3 e cAMP (TREs, thyroid response elements e CREs, cAMP response elements), utilizando como referência o promotor da UCP1, SERCA2 e gliceraldeído 3-fosfato desidrogenase. Selecionamos somente os promotores com estudo funcional prévio in vitro. RESULTADOS: 29/56 enzimas apresentaram TREs em suas regiões regulatórias, parte com escore > 0,80 (melhor valor preditivo 1): citrato sintase, fosfoglucose isomerase, succinato desidrogenase A/C, UCP3, UCP2, UCP4, UCP5, fosfoglicerato mutase, gliceraldeído 3-P desidrogenase, glucoquinase, malato desidrogenase, acil-CoA transferase (tiolase), citocromo a3, e lactato desidrogenase; parte desconhecida como regulada por T3. CONCLUSÃO: Os resultados do presente estudo apontam para novos genes regulados por T3 e cAMP e, por conseguinte, sua contribuição na regulação da termogênese.Universidade Federal de São Paulo (UNIFESP)UNIFESP, EPMSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de Endocrinologia e MetabologiaUniversidade Federal de São Paulo (UNIFESP)Dorsa, Karina Kores [UNIFESP]Santos, Michelle Venâncio Dos [UNIFESP]Dias-da-Silva, Magnus Régios [UNIFESP]2015-06-14T13:41:41Z2015-06-14T13:41:41Z2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion381-389application/pdfhttp://dx.doi.org/10.1590/S0004-27302010000400007Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 54, n. 4, p. 381-389, 2010.10.1590/S0004-27302010000400007S0004-27302010000400007.pdf0004-2730S0004-27302010000400007http://repositorio.unifesp.br/handle/11600/5754WOS:000279409500007engArquivos Brasileiros de Endocrinologia & Metabologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T15:10:34Zoai:repositorio.unifesp.br/:11600/5754Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T15:10:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
Ampliando a participação dos genes responsivos a T3 e cAMP na regulação da termogênese gerada pelas vias de oxidação biológica
title Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
spellingShingle Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
Dorsa, Karina Kores [UNIFESP]
Thermogenesis
T3R
CREB
genomic regulation
fuel oxidation
Termogênese
T3R
CREB
regulação gênica
oxidação biológica
title_short Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
title_full Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
title_fullStr Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
title_full_unstemmed Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
title_sort Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
author Dorsa, Karina Kores [UNIFESP]
author_facet Dorsa, Karina Kores [UNIFESP]
Santos, Michelle Venâncio Dos [UNIFESP]
Dias-da-Silva, Magnus Régios [UNIFESP]
author_role author
author2 Santos, Michelle Venâncio Dos [UNIFESP]
Dias-da-Silva, Magnus Régios [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Dorsa, Karina Kores [UNIFESP]
Santos, Michelle Venâncio Dos [UNIFESP]
Dias-da-Silva, Magnus Régios [UNIFESP]
dc.subject.por.fl_str_mv Thermogenesis
T3R
CREB
genomic regulation
fuel oxidation
Termogênese
T3R
CREB
regulação gênica
oxidação biológica
topic Thermogenesis
T3R
CREB
genomic regulation
fuel oxidation
Termogênese
T3R
CREB
regulação gênica
oxidação biológica
description OBJECTIVE: We sought to identify glycolysis, glycogenolysis, lipolysis, Krebs cycle, respiratory chain, and oxidative phosphorylation enzymes simultaneously regulated by T3 and cAMP. MATERIALS AND METHODS: We performed in silico analysis of 56 promoters to search for cis-cAMP (CREB) and cis-thyroid (TRE) response elements, considering UCP1, SERCA2 and glyceraldehyde 3-phosphate dehydrogenase as reference. Only regulatory regions with prior in vitro validation were selected. RESULTS: 29/56 enzymes presented potential TREs in their regulatory sequence, and some scored over 0.80 (better predictive value 1): citrate synthase, phosphoglucose isomerase, succinate dehydrogenases A/C, UCP3, UCP2, UCP4, UCP5, phosphoglycerate mutase, glyceraldehyde 3-P dehydrogenase, glucokinase, malate dehydrogenase, acyl-CoA transferase (thiolase), cytochrome a3, and lactate dehydrogenase. Moreover, some enzymes have not yet been described in the literature as genomically regulated by T3. CONCLUSION: Our results point to other enzymes which may possibly be regulated by T3 and CREB, and speculate their joint roles in contributing to the optimal thermogenic acclimation.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
2015-06-14T13:41:41Z
2015-06-14T13:41:41Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-27302010000400007
Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 54, n. 4, p. 381-389, 2010.
10.1590/S0004-27302010000400007
S0004-27302010000400007.pdf
0004-2730
S0004-27302010000400007
http://repositorio.unifesp.br/handle/11600/5754
WOS:000279409500007
url http://dx.doi.org/10.1590/S0004-27302010000400007
http://repositorio.unifesp.br/handle/11600/5754
identifier_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia. Sociedade Brasileira de Endocrinologia e Metabologia, v. 54, n. 4, p. 381-389, 2010.
10.1590/S0004-27302010000400007
S0004-27302010000400007.pdf
0004-2730
S0004-27302010000400007
WOS:000279409500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 381-389
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
publisher.none.fl_str_mv Sociedade Brasileira de Endocrinologia e Metabologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268292286644224

Registros relacionados