Desenvolvimento e Validação de Um Método Imunofluorométrico Para a Pesquisa de Anticorpos Antiperoxidase Tiroidiana no Soro

• Autor(a) principal: Kasamatsu, Teresa S. [UNIFESP]
• Data de Publicação: 2002
• Outros Autores: Maciel, Rui Monteiro de Barros [UNIFESP], Vieira, José Gilberto H. [UNIFESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0004-27302002000200009; http://repositorio.unifesp.br/handle/11600/1402
• topic: Anti-thyroid antibodies; Anti-thyroperoxidase antibodies; Anti-thyroglobulin antibodies; Autoimune thyroid diseases; Anticorpos antitiroidianos; Anticorpos antiperoxidase tiroidiana; Anticorpos antitiroglobulina; Doenças auto-imunes da tiróide

We developed an assay in which highly purified human TPO is adsorbed to microtiter plate wells; after incubation with the study serum samples, presence of specific antibodies is revealed by a monoclonal europium-labeled antibody against human IgG. The sensitivity of the test is 0.6U/mL, and the reproducibility quite acceptable. We analyzed serum samples from: 188 normal subjects of both sexes, 74 of whom >65 years of age; 54 patients with Graves' disease (GD) and 13 with Hashimoto thyroiditis (HT). Based on 97 routine samples, the method was compared to a commercial RIA, and a high concordance was found, with a kappa index of 0.959. The definition of a cut-off value for the presence of anti-TPO antibodies was based on a ROC curve and was set at 10U/mL. Positive anti-TPO values were found in 9.6% of the normal subjects up to 64 years of age, and in 16.5% of those >65 years. Patients with GD presented 85.2% of positivity, and those with HT showed 100% positivity. Thus, we described a sensitive and specific easy to perform assay, for detection of autoantibodies against thyroid peroxidase in patients with thyroid autoimmune diseases.