Integration of collagen matrices into the urethra when implanted as onlay graft
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36254 http://dx.doi.org/10.1590/S1677-5538.IBJU.2013.03.16 |
Resumo: | Objective: To assess the integration of decellularized heterologous collagen matrices into the urethra, when implanted with no cells or when seeded with autologous smooth muscle cells.Materials and Methods: Eighteen New Zealand rabbits were randomly assigned to two groups: Group I (n = 9) - animals undergoing urethral segment resection with interposition of a patch of heterologous collagen matrix seeded with autologous smooth muscle cells; Group II (n = 9) - animals undergoing resection of a urethral segment with interposition of a decellularized heterologous collagen matrix patch. Two animals from each group were sacrificed on postoperative days seven, fourteen and twenty-eight; three animals from each group were sacrificed at the end of three postoperative months. At the end of the third month one animal from each group underwent urethroscopy for urethral integrity assessment and one animal from each group had its microcirculation image captured by a SDF device (Side-stream Dark Field - Microscan Analysis Software). One animal from each group in each euthanasia period underwent cystourethrography so as the urethra could be viewed at flow time. the matrices integration was assessed through histological examination using hematoxylin and eosin (H&E), Masson trichrome (MT), Picrosirius red and Von Willebrand staining. in a blind study with two pathologists all the slides were studied.Results: the matrices whether seeded or not with autologous muscle cells were able to restore the architecture of the urethra, but were eliminated from the first week on, before incorporation. Microcirculation of the neourethra, at the end of the third month, showed the same characteristics as a normal urethra in both groups of animals.Conclusion: Natural heterologous matrices implanted in the urethra as onlay graft were not incorporated into its walls but were able to fully restore the cell architecture of the organ, regardless of being seeded or not with autologous muscle cells. |
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Repositório Institucional da UNIFESP |
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3465 |
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Sayeg, Kleber [UNIFESP]Freitas-Filho, Luiz G. [UNIFESP]Waitzberg, Angela Flavia Logullo [UNIFESP]Arias, Victor Eduardo Arrua [UNIFESP]Laks, Marcus [UNIFESP]Egydio, Fernanda Mattos [UNIFESP]Oliveira, Andreia Silva [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:31:38Z2016-01-24T14:31:38Z2013-05-01International Braz J Urol. Rio de Janeiro: Brazilian Soc Urol, v. 39, n. 3, p. 414-423, 2013.1677-5538http://repositorio.unifesp.br/handle/11600/36254http://dx.doi.org/10.1590/S1677-5538.IBJU.2013.03.16S1677-5538.IBJU.2013.03.1610.1590/S1677-5538.IBJU.2013.03.16WOS:000323502700018Objective: To assess the integration of decellularized heterologous collagen matrices into the urethra, when implanted with no cells or when seeded with autologous smooth muscle cells.Materials and Methods: Eighteen New Zealand rabbits were randomly assigned to two groups: Group I (n = 9) - animals undergoing urethral segment resection with interposition of a patch of heterologous collagen matrix seeded with autologous smooth muscle cells; Group II (n = 9) - animals undergoing resection of a urethral segment with interposition of a decellularized heterologous collagen matrix patch. Two animals from each group were sacrificed on postoperative days seven, fourteen and twenty-eight; three animals from each group were sacrificed at the end of three postoperative months. At the end of the third month one animal from each group underwent urethroscopy for urethral integrity assessment and one animal from each group had its microcirculation image captured by a SDF device (Side-stream Dark Field - Microscan Analysis Software). One animal from each group in each euthanasia period underwent cystourethrography so as the urethra could be viewed at flow time. the matrices integration was assessed through histological examination using hematoxylin and eosin (H&E), Masson trichrome (MT), Picrosirius red and Von Willebrand staining. in a blind study with two pathologists all the slides were studied.Results: the matrices whether seeded or not with autologous muscle cells were able to restore the architecture of the urethra, but were eliminated from the first week on, before incorporation. Microcirculation of the neourethra, at the end of the third month, showed the same characteristics as a normal urethra in both groups of animals.Conclusion: Natural heterologous matrices implanted in the urethra as onlay graft were not incorporated into its walls but were able to fully restore the cell architecture of the organ, regardless of being seeded or not with autologous muscle cells.Universidade Federal de São Paulo, Dept Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Nephrol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilWeb of Science414-423engBrazilian Soc UrolInternational Braz J UrolUrethraSurgical Procedures, OperativeTissue EngineeringMicrocirculationIntegration of collagen matrices into the urethra when implanted as onlay graftinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/362542022-09-27 11:31:15.845metadata only accessoai:repositorio.unifesp.br:11600/36254Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T14:31:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Integration of collagen matrices into the urethra when implanted as onlay graft |
title |
Integration of collagen matrices into the urethra when implanted as onlay graft |
spellingShingle |
Integration of collagen matrices into the urethra when implanted as onlay graft Sayeg, Kleber [UNIFESP] Urethra Surgical Procedures, Operative Tissue Engineering Microcirculation |
title_short |
Integration of collagen matrices into the urethra when implanted as onlay graft |
title_full |
Integration of collagen matrices into the urethra when implanted as onlay graft |
title_fullStr |
Integration of collagen matrices into the urethra when implanted as onlay graft |
title_full_unstemmed |
Integration of collagen matrices into the urethra when implanted as onlay graft |
title_sort |
Integration of collagen matrices into the urethra when implanted as onlay graft |
author |
Sayeg, Kleber [UNIFESP] |
author_facet |
Sayeg, Kleber [UNIFESP] Freitas-Filho, Luiz G. [UNIFESP] Waitzberg, Angela Flavia Logullo [UNIFESP] Arias, Victor Eduardo Arrua [UNIFESP] Laks, Marcus [UNIFESP] Egydio, Fernanda Mattos [UNIFESP] Oliveira, Andreia Silva [UNIFESP] |
author_role |
author |
author2 |
Freitas-Filho, Luiz G. [UNIFESP] Waitzberg, Angela Flavia Logullo [UNIFESP] Arias, Victor Eduardo Arrua [UNIFESP] Laks, Marcus [UNIFESP] Egydio, Fernanda Mattos [UNIFESP] Oliveira, Andreia Silva [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Sayeg, Kleber [UNIFESP] Freitas-Filho, Luiz G. [UNIFESP] Waitzberg, Angela Flavia Logullo [UNIFESP] Arias, Victor Eduardo Arrua [UNIFESP] Laks, Marcus [UNIFESP] Egydio, Fernanda Mattos [UNIFESP] Oliveira, Andreia Silva [UNIFESP] |
dc.subject.eng.fl_str_mv |
Urethra Surgical Procedures, Operative Tissue Engineering Microcirculation |
topic |
Urethra Surgical Procedures, Operative Tissue Engineering Microcirculation |
description |
Objective: To assess the integration of decellularized heterologous collagen matrices into the urethra, when implanted with no cells or when seeded with autologous smooth muscle cells.Materials and Methods: Eighteen New Zealand rabbits were randomly assigned to two groups: Group I (n = 9) - animals undergoing urethral segment resection with interposition of a patch of heterologous collagen matrix seeded with autologous smooth muscle cells; Group II (n = 9) - animals undergoing resection of a urethral segment with interposition of a decellularized heterologous collagen matrix patch. Two animals from each group were sacrificed on postoperative days seven, fourteen and twenty-eight; three animals from each group were sacrificed at the end of three postoperative months. At the end of the third month one animal from each group underwent urethroscopy for urethral integrity assessment and one animal from each group had its microcirculation image captured by a SDF device (Side-stream Dark Field - Microscan Analysis Software). One animal from each group in each euthanasia period underwent cystourethrography so as the urethra could be viewed at flow time. the matrices integration was assessed through histological examination using hematoxylin and eosin (H&E), Masson trichrome (MT), Picrosirius red and Von Willebrand staining. in a blind study with two pathologists all the slides were studied.Results: the matrices whether seeded or not with autologous muscle cells were able to restore the architecture of the urethra, but were eliminated from the first week on, before incorporation. Microcirculation of the neourethra, at the end of the third month, showed the same characteristics as a normal urethra in both groups of animals.Conclusion: Natural heterologous matrices implanted in the urethra as onlay graft were not incorporated into its walls but were able to fully restore the cell architecture of the organ, regardless of being seeded or not with autologous muscle cells. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:31:38Z |
dc.date.available.fl_str_mv |
2016-01-24T14:31:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
International Braz J Urol. Rio de Janeiro: Brazilian Soc Urol, v. 39, n. 3, p. 414-423, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/36254 http://dx.doi.org/10.1590/S1677-5538.IBJU.2013.03.16 |
dc.identifier.issn.none.fl_str_mv |
1677-5538 |
dc.identifier.scielo.none.fl_str_mv |
S1677-5538.IBJU.2013.03.16 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1677-5538.IBJU.2013.03.16 |
dc.identifier.wos.none.fl_str_mv |
WOS:000323502700018 |
identifier_str_mv |
International Braz J Urol. Rio de Janeiro: Brazilian Soc Urol, v. 39, n. 3, p. 414-423, 2013. 1677-5538 S1677-5538.IBJU.2013.03.16 10.1590/S1677-5538.IBJU.2013.03.16 WOS:000323502700018 |
url |
http://repositorio.unifesp.br/handle/11600/36254 http://dx.doi.org/10.1590/S1677-5538.IBJU.2013.03.16 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
International Braz J Urol |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
414-423 |
dc.publisher.none.fl_str_mv |
Brazilian Soc Urol |
publisher.none.fl_str_mv |
Brazilian Soc Urol |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764172157517824 |