Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | https://dx.doi.org/10.1590/S0102-86502009000500011 https://repositorio.unifesp.br/handle/11600/5282 |
Resumo: | PURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest. |
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Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbitsEstudo comparativo da técnica para a obtenção de células tronco pela coleta de medula óssea entre a crista ilíaca e epífise do fêmur em coelhosBone MarrowAdult Stem CellsRabbitsMedula ÓsseaCélulas-Tronco AdultasCoelhosPURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.OBJETIVO: Avaliar a técnica mais promissora para a coleta de células tronco adultas de medula óssea de coelhos para a utilização do mesmo como modelo experimental na medicina regenerativa. MÉTODOS: Foram utilizados 30 coelhos divididos em 2 grupos: GRUPO A, n=8, onde realizamos a coleta de sangue de medula óssea (MO) da crista ilíaca e grupo B, n=22, onde realizamos a coleta de sangue da medula óssea da epífise do fêmur. Após as coletas, realizamos a separação das células mononucleadas através do gradiente de densidade (Ficoll-Hystopaque). Através da câmara de Neubauer realizamos a contagem das células mononucleadas por ml. Testamos a viabilidade celular através do método Tripan Blue. RESULTADOS: Na coleta de sangue de MO na crista ilíaca obtivemos a média de 1 ml durante 1 hora de procedimento cirúrgico, obtendo a quantidade de 3,6 .10(6) células/ml, enquanto que a punção na epífise do fêmur obtivemos a média de 3 ml durante 30 minutos de procedimento cirúrgico obtendo a quantidade de 11,79.10(6) cél./ml diminuindo o óbito dos animais. CONCLUSÃO: A análise para a obtenção de maior número de células mononucleadas/ml de sangue de medula óssea de coelho foi mais satisfatória na região da epífise do fêmur em comparação com a crista ilíaca.Stem Cell Research InstituteSacred Heart UniversityFederal University of São PauloUniversidade Federal de São Paulo (UNIFESP) Department of Surgery Division Experimental SurgeryUniversidade Federal de São Paulo (UNIFESP) Department of OtorhinolaryngologyUniversidade Federal de São Paulo (UNIFESP) Department of Molecular BiologyLusiada University of Santos Department Plastic SurgeryUNIFESP, Department of Surgery Division Experimental SurgeryUNIFESP, Department of OtorhinolaryngologyUNIFESP, Department of Molecular BiologySciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaStem Cell Research InstituteSacred Heart UniversityUniversidade Federal de São Paulo (UNIFESP)Lusiada University of Santos Department Plastic SurgeryEça, Lilian Piñero MarcolinRamalho, Renata BelmonteOliveira, Isis Sousa [UNIFESP]Gomes, Paulo Oliveira [UNIFESP]Pontes, Paulo [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Mazzetti, Marcelo Paulo Vaccari [UNIFESP]2015-06-14T13:41:12Z2015-06-14T13:41:12Z2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion400-404application/pdfhttps://dx.doi.org/10.1590/S0102-86502009000500011Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 24, n. 5, p. 400-404, 2009.10.1590/S0102-86502009000500011S0102-86502009000500011.pdf0102-8650S0102-86502009000500011https://repositorio.unifesp.br/handle/11600/5282WOS:000271115100011engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T18:10:09Zoai:repositorio.unifesp.br/:11600/5282Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T18:10:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits Estudo comparativo da técnica para a obtenção de células tronco pela coleta de medula óssea entre a crista ilíaca e epífise do fêmur em coelhos |
| title |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| spellingShingle |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits Eça, Lilian Piñero Marcolin Bone Marrow Adult Stem Cells Rabbits Medula Óssea Células-Tronco Adultas Coelhos |
| title_short |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| title_full |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| title_fullStr |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| title_full_unstemmed |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| title_sort |
Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits |
| author |
Eça, Lilian Piñero Marcolin |
| author_facet |
Eça, Lilian Piñero Marcolin Ramalho, Renata Belmonte Oliveira, Isis Sousa [UNIFESP] Gomes, Paulo Oliveira [UNIFESP] Pontes, Paulo [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] Mazzetti, Marcelo Paulo Vaccari [UNIFESP] |
| author_role |
author |
| author2 |
Ramalho, Renata Belmonte Oliveira, Isis Sousa [UNIFESP] Gomes, Paulo Oliveira [UNIFESP] Pontes, Paulo [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] Mazzetti, Marcelo Paulo Vaccari [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Stem Cell Research Institute Sacred Heart University Universidade Federal de São Paulo (UNIFESP) Lusiada University of Santos Department Plastic Surgery |
| dc.contributor.author.fl_str_mv |
Eça, Lilian Piñero Marcolin Ramalho, Renata Belmonte Oliveira, Isis Sousa [UNIFESP] Gomes, Paulo Oliveira [UNIFESP] Pontes, Paulo [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] Mazzetti, Marcelo Paulo Vaccari [UNIFESP] |
| dc.subject.por.fl_str_mv |
Bone Marrow Adult Stem Cells Rabbits Medula Óssea Células-Tronco Adultas Coelhos |
| topic |
Bone Marrow Adult Stem Cells Rabbits Medula Óssea Células-Tronco Adultas Coelhos |
| description |
PURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-10-01 2015-06-14T13:41:12Z 2015-06-14T13:41:12Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S0102-86502009000500011 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 24, n. 5, p. 400-404, 2009. 10.1590/S0102-86502009000500011 S0102-86502009000500011.pdf 0102-8650 S0102-86502009000500011 https://repositorio.unifesp.br/handle/11600/5282 WOS:000271115100011 |
| url |
https://dx.doi.org/10.1590/S0102-86502009000500011 https://repositorio.unifesp.br/handle/11600/5282 |
| identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 24, n. 5, p. 400-404, 2009. 10.1590/S0102-86502009000500011 S0102-86502009000500011.pdf 0102-8650 S0102-86502009000500011 WOS:000271115100011 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Acta Cirurgica Brasileira |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
400-404 application/pdf |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268271151546368 |