Captive wild birds as reservoirs of enteropathogenic E-coli (EPEC) and Shiga-toxin producing E-coli (STEC)

• Autor(a) principal: Sanches, Lilian Aparecida
• Data de Publicação: 2017
• Outros Autores: Gomes, Marcelo da Silva, Friciello Teixeira, Rodrigo Hidalgo, Vieira Cunha, Marcos Paulo, Xavier de Oliveira, Maria Gabriela, Midolli Vieira, Monica Aparecida [UNIFESP], Tardelli Gomes, Tania Aparecida [UNIFESP], Knobl, Terezinha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1016/j.bjm.2017.03.003; https://repositorio.unifesp.br/handle/11600/57231
• topic: Diarrheagenic E. coli; Atypical EPEC; STEC; Avian diseases

Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of Sao Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp (EPEC), stxl and stx2 for STEC. The results of these tests revealed 23/401 (5.74%) positive strains for eae gene, 16/401 positive strains for the bfp gene (3.99%) and 3/401 positive for stx2 gene (0.75%) distributed among the orders of Psittaciformes, Strigiformes and Columbiformes. None of strains were positive for stxl gene. These data reveal the infection by STEC, typical and atypical EPEC in captive birds. The frequency of these pathotypes is low and restricted to few orders, but the data suggest the potential public health risk that these birds represent as reservoirs of diarrheagenic E. coli. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.