False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos

Detalhes bibliográficos
Autor(a) principal: Deps, P. D.
Data de Publicação: 2004
Outros Autores: Michalany, Nilceo S. [UNIFESP], Tomimori-Yamashita, Jane [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://ijl.ilsl.br/detalhe_artigo.php?id=ODE=#
https://repositorio.unifesp.br/handle/11600/27902
Resumo: The authors studied 66 wild nine-banded armadillos from Brazil. the ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. the AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. the authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos.
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spelling False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillosThe authors studied 66 wild nine-banded armadillos from Brazil. the ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. the AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. the authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos.Fed Univ Espirito Santo, Dept Social Med, Infect Dis Unit, Vitoria, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Dermatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Dermatol, São Paulo, BrazilWeb of ScienceInt Journal LeprosyFed Univ Espirito SantoUniversidade Federal de São Paulo (UNIFESP)Deps, P. D.Michalany, Nilceo S. [UNIFESP]Tomimori-Yamashita, Jane [UNIFESP]2016-01-24T12:37:20Z2016-01-24T12:37:20Z2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion327-330http://ijl.ilsl.br/detalhe_artigo.php?id=ODE=#International Journal of Leprosy and Other Mycobacterial Diseases. Greenville: Int Journal Leprosy, v. 72, n. 3, p. 327-330, 2004.10.1489/0020-7349(2004)72<327:FPROTI>2.0.CO;20148-916Xhttps://repositorio.unifesp.br/handle/11600/27902WOS:000224716500010engInternational Journal of Leprosy and Other Mycobacterial Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-11-22T15:56:18Zoai:repositorio.unifesp.br/:11600/27902Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-11-22T15:56:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
title False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
spellingShingle False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
Deps, P. D.
title_short False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
title_full False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
title_fullStr False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
title_full_unstemmed False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
title_sort False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos
author Deps, P. D.
author_facet Deps, P. D.
Michalany, Nilceo S. [UNIFESP]
Tomimori-Yamashita, Jane [UNIFESP]
author_role author
author2 Michalany, Nilceo S. [UNIFESP]
Tomimori-Yamashita, Jane [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Fed Univ Espirito Santo
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Deps, P. D.
Michalany, Nilceo S. [UNIFESP]
Tomimori-Yamashita, Jane [UNIFESP]
description The authors studied 66 wild nine-banded armadillos from Brazil. the ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. the AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. the authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
2016-01-24T12:37:20Z
2016-01-24T12:37:20Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://ijl.ilsl.br/detalhe_artigo.php?id=ODE=#
International Journal of Leprosy and Other Mycobacterial Diseases. Greenville: Int Journal Leprosy, v. 72, n. 3, p. 327-330, 2004.
10.1489/0020-7349(2004)72<327:FPROTI>2.0.CO;2
0148-916X
https://repositorio.unifesp.br/handle/11600/27902
WOS:000224716500010
url http://ijl.ilsl.br/detalhe_artigo.php?id=ODE=#
https://repositorio.unifesp.br/handle/11600/27902
identifier_str_mv International Journal of Leprosy and Other Mycobacterial Diseases. Greenville: Int Journal Leprosy, v. 72, n. 3, p. 327-330, 2004.
10.1489/0020-7349(2004)72<327:FPROTI>2.0.CO;2
0148-916X
WOS:000224716500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Leprosy and Other Mycobacterial Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 327-330
dc.publisher.none.fl_str_mv Int Journal Leprosy
publisher.none.fl_str_mv Int Journal Leprosy
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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