Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57587 http://dx.doi.org/10.1590/0102-33062016abb0149 |
Resumo: | To describe potential intraspecific variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fluorescence assay. Analysis using the probe ELF-97 (R) provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphate-enriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. The P. tricornutum strains exhibited differences in their development when incubated in the phosphate-enriched media. The development of the Ub7 strain differed by exhibiting "luxury uptake" and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. The Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecific differences in alkaline phosphatase activity, and hence differences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under different external phosphate concentrations. |
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de Lima, Domenica TeixeiraPiedras, Fernanda ReinhardtNogueira, Flavia Previero [UNIFESP]Richard, Eduardo da CunhaCastaldi Tocci, Bianca ReisBarrerea Alba, Jose JuanOliveira Moser, Gleyci Aparecida2020-08-14T13:44:19Z2020-08-14T13:44:19Z2016Acta Botanica Brasilica. Sao Paulo Sp, v. 30, n. 3, p. 445-454, 2016.0102-3306https://repositorio.unifesp.br/handle/11600/57587http://dx.doi.org/10.1590/0102-33062016abb0149WOS000385543100011.pdf10.1590/0102-33062016abb0149WOS:000385543100011To describe potential intraspecific variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fluorescence assay. Analysis using the probe ELF-97 (R) provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphate-enriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. The P. tricornutum strains exhibited differences in their development when incubated in the phosphate-enriched media. The development of the Ub7 strain differed by exhibiting "luxury uptake" and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. The Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecific differences in alkaline phosphatase activity, and hence differences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under different external phosphate concentrations.Univ Estado Rio de Janeiro, Fac Oceanog, Lab Ecol & Cult Fitoplancton Marinho, BR-20550900 Rio De Janeiro, RJ, BrazilUniv Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, BR-11030400 Santos, SP, BrazilInst Nacl Pesquisas Espaciais, Km 39, BR-12630000 Cachoeira Paulista, SP, BrazilUniv Fed Sao Paulo, Dept Ciencias Mar, Campus Baixada Santista, BR-11030400 Santos, SP, BrazilWeb of Science445-454engSoc Botanica BrasilActa Botanica Brasilicaalkaline phosphataseauxotrophymarine diatomorganic phosphorus incorporationphosphate limitationIntraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSao Paulo Sp303info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000385543100011.pdfapplication/pdf1514563${dspace.ui.url}/bitstream/11600/57587/1/WOS000385543100011.pdf07d709d297d31c99bba9a986af3d9942MD51open accessTEXTWOS000385543100011.pdf.txtWOS000385543100011.pdf.txtExtracted texttext/plain47786${dspace.ui.url}/bitstream/11600/57587/5/WOS000385543100011.pdf.txt1341c5fbb4df223a16a23f64359e0721MD55open accessTHUMBNAILWOS000385543100011.pdf.jpgWOS000385543100011.pdf.jpgIM Thumbnailimage/jpeg7414${dspace.ui.url}/bitstream/11600/57587/7/WOS000385543100011.pdf.jpge4c8a0363fea908c1e381ea954b989feMD57open access11600/575872023-06-05 19:40:00.732open accessoai:repositorio.unifesp.br:11600/57587Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:40Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
title |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
spellingShingle |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) de Lima, Domenica Teixeira alkaline phosphatase auxotrophy marine diatom organic phosphorus incorporation phosphate limitation |
title_short |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
title_full |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
title_fullStr |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
title_full_unstemmed |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
title_sort |
Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) |
author |
de Lima, Domenica Teixeira |
author_facet |
de Lima, Domenica Teixeira Piedras, Fernanda Reinhardt Nogueira, Flavia Previero [UNIFESP] Richard, Eduardo da Cunha Castaldi Tocci, Bianca Reis Barrerea Alba, Jose Juan Oliveira Moser, Gleyci Aparecida |
author_role |
author |
author2 |
Piedras, Fernanda Reinhardt Nogueira, Flavia Previero [UNIFESP] Richard, Eduardo da Cunha Castaldi Tocci, Bianca Reis Barrerea Alba, Jose Juan Oliveira Moser, Gleyci Aparecida |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
de Lima, Domenica Teixeira Piedras, Fernanda Reinhardt Nogueira, Flavia Previero [UNIFESP] Richard, Eduardo da Cunha Castaldi Tocci, Bianca Reis Barrerea Alba, Jose Juan Oliveira Moser, Gleyci Aparecida |
dc.subject.eng.fl_str_mv |
alkaline phosphatase auxotrophy marine diatom organic phosphorus incorporation phosphate limitation |
topic |
alkaline phosphatase auxotrophy marine diatom organic phosphorus incorporation phosphate limitation |
description |
To describe potential intraspecific variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fluorescence assay. Analysis using the probe ELF-97 (R) provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphate-enriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. The P. tricornutum strains exhibited differences in their development when incubated in the phosphate-enriched media. The development of the Ub7 strain differed by exhibiting "luxury uptake" and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. The Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecific differences in alkaline phosphatase activity, and hence differences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under different external phosphate concentrations. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-14T13:44:19Z |
dc.date.available.fl_str_mv |
2020-08-14T13:44:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Botanica Brasilica. Sao Paulo Sp, v. 30, n. 3, p. 445-454, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57587 http://dx.doi.org/10.1590/0102-33062016abb0149 |
dc.identifier.issn.none.fl_str_mv |
0102-3306 |
dc.identifier.file.none.fl_str_mv |
WOS000385543100011.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1590/0102-33062016abb0149 |
dc.identifier.wos.none.fl_str_mv |
WOS:000385543100011 |
identifier_str_mv |
Acta Botanica Brasilica. Sao Paulo Sp, v. 30, n. 3, p. 445-454, 2016. 0102-3306 WOS000385543100011.pdf 10.1590/0102-33062016abb0149 WOS:000385543100011 |
url |
https://repositorio.unifesp.br/handle/11600/57587 http://dx.doi.org/10.1590/0102-33062016abb0149 |
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eng |
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Acta Botanica Brasilica |
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openAccess |
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445-454 |
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Sao Paulo Sp |
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Soc Botanica Brasil |
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Soc Botanica Brasil |
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