Effect of hyperbaric oxygen therapy combined with autologous platelet concentrate applied in rabbit fibula fraction healing

• Autor(a) principal: Neves, Paulo Cesar Fagundes
• Data de Publicação: 2013
• Outros Autores: Abib, Simone de Campos Vieira [UNIFESP], Neves, Rogerio Fagundes [UNIFESP], Pircchio, Oronzo, Saad, Karen Ruggeri [UNIFESP], Saad, Paulo Fernandes [UNIFESP], Simoes, Ricardo Santos [UNIFESP], Moreira, Marcia Bento, Laurino, Cristiano Frota de Souza
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300000spgj
• DOI: 10.6061/clinics/2013(09)11
• Texto Completo: http://dx.doi.org/10.6061/clinics/2013(09)11; http://repositorio.unifesp.br/handle/11600/7947
• topic: Hyperbaric Oxygenation; Platelet-rich Plasma; Fibula, Fracture Consolidation

OBJECTIVES: The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures. METHODS: A total of 128 male New Zealand albino rabbits, between 6-8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red. RESULTS: Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group. CONCLUSION: Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.