SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57962 http://dx.doi.org/10.1038/srep21752 |
Resumo: | High glucose concentration in the airway surface liquid (ASL) is an important feature of diabetes that predisposes to respiratory infections. We investigated the role of alveolar epithelial SGLT1 activity on ASL glucose concentration and bacterial proliferation. Non-diabetic and diabetic rats were intranasally treated with saline, isoproterenol (to increase SGLT1 activity) or phlorizin (to decrease SGLT1 activity); 2 hours later, glucose concentration and bacterial proliferation (methicillin-resistant Sthaphylococcus aureus, MRSA and Pseudomonas aeruginosa, P. aeruginosa) were analyzed in bronchoalveolar lavage (BAL); and alveolar SGLT1 was analyzed by immunohistochemistry. BAL glucose concentration and bacterial proliferation increased in diabetic animals: isoproterenol stimulated SGLT1 migration to luminal membrane, and reduced (50%) the BAL glucose concentration; whereas phlorizin increased the BAL glucose concentration (100%). These regulations were accompanied by parallel changes of in vitro MRSA and P. aeruginosa proliferation in BAL (r = 0.9651 and r = 0.9613, respectively, Pearson correlation). The same regulations were observed in in vivo P. aeruginosa proliferation. In summary, the results indicate a relationship among SGLT1 activity, ASL glucose concentration and pulmonary bacterial proliferation. Besides, the study highlights that, in situations of pulmonary infection risk, such as in diabetic subjects, increased SGLT1 activity may prevent bacterial proliferation whereas decreased SGLT1 activity can exacerbate it. |
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Oliveira, Tales Lyra [UNIFESP]Candeia-Medeiros, NavyllaCavalcante-Araujo, Polliane M.Melo, Igor SantanaFavaro-Pipi, ElaineFatima, Luciana Alves [UNIFESP]Rocha, Antonio Augusto [UNIFESP]Goulart, Luiz RicardoMachado, Ubiratan Fabres [UNIFESP]Campos, Ruy R. [UNIFESP]Sabino-Silva, Robinson2020-08-21T17:00:22Z2020-08-21T17:00:22Z2016Scientific Reports. London, v. 6, p. -, 2016.2045-2322https://repositorio.unifesp.br/handle/11600/57962http://dx.doi.org/10.1038/srep21752WOS000370631800001.pdf10.1038/srep21752WOS:000370631800001High glucose concentration in the airway surface liquid (ASL) is an important feature of diabetes that predisposes to respiratory infections. We investigated the role of alveolar epithelial SGLT1 activity on ASL glucose concentration and bacterial proliferation. Non-diabetic and diabetic rats were intranasally treated with saline, isoproterenol (to increase SGLT1 activity) or phlorizin (to decrease SGLT1 activity); 2 hours later, glucose concentration and bacterial proliferation (methicillin-resistant Sthaphylococcus aureus, MRSA and Pseudomonas aeruginosa, P. aeruginosa) were analyzed in bronchoalveolar lavage (BAL); and alveolar SGLT1 was analyzed by immunohistochemistry. BAL glucose concentration and bacterial proliferation increased in diabetic animals: isoproterenol stimulated SGLT1 migration to luminal membrane, and reduced (50%) the BAL glucose concentration; whereas phlorizin increased the BAL glucose concentration (100%). These regulations were accompanied by parallel changes of in vitro MRSA and P. aeruginosa proliferation in BAL (r = 0.9651 and r = 0.9613, respectively, Pearson correlation). The same regulations were observed in in vivo P. aeruginosa proliferation. In summary, the results indicate a relationship among SGLT1 activity, ASL glucose concentration and pulmonary bacterial proliferation. Besides, the study highlights that, in situations of pulmonary infection risk, such as in diabetic subjects, increased SGLT1 activity may prevent bacterial proliferation whereas decreased SGLT1 activity can exacerbate it.CAPESFederal University of UberlandiaFAPEMIGFAPEALFAPESPFAPEAL fellowshipUniv Fed Alagoas, Inst Biol Sci & Hlth, Alagoas, BrazilUniv Fed Sao Paulo, Dept Physiol, Sao Paulo, BrazilUniv Fed Uberlandia, Natl Reference Ctr Leprosy & Sanit Dermatol, Uberlandia, MG, BrazilUniv Sao Paulo, Inst Biomed Sci, Dept Physiol, Sao Paulo, BrazilUniv Fed Uberlandia, Inst Genet & Biochem, Uberlandia, MG, BrazilUniv Calif Davis, Dept Med Microbiol & Immunol, Davis, CA USAUniv Fed Uberlandia, Inst Biomed Sci, Dept Physiol, Uberlandia, MG, BrazilUniv Fed Sao Paulo, Dept Physiol, Sao Paulo, BrazilFAPESP: 201/04831-1Web of Science-engNature Publishing GroupScientific ReportsSGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon6info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000370631800001.pdfapplication/pdf1763617${dspace.ui.url}/bitstream/11600/57962/1/WOS000370631800001.pdf66d847b07993b23b2e71793ebb538becMD51open accessTEXTWOS000370631800001.pdf.txtWOS000370631800001.pdf.txtExtracted texttext/plain45217${dspace.ui.url}/bitstream/11600/57962/2/WOS000370631800001.pdf.txteabe006b1cb12f738af7d6951018030bMD52open accessTHUMBNAILWOS000370631800001.pdf.jpgWOS000370631800001.pdf.jpgIM Thumbnailimage/jpeg7418${dspace.ui.url}/bitstream/11600/57962/4/WOS000370631800001.pdf.jpge0225f05e218bf2b7c6f22e34482ace4MD54open access11600/579622022-07-31 19:28:03.849open accessoai:repositorio.unifesp.br:11600/57962Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-31T22:28:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
title |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
spellingShingle |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation Oliveira, Tales Lyra [UNIFESP] |
title_short |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
title_full |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
title_fullStr |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
title_full_unstemmed |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
title_sort |
SGLT1 activity in lung alveolar cells of diabetic rats modulates airway surface liquid glucose concentration and bacterial proliferation |
author |
Oliveira, Tales Lyra [UNIFESP] |
author_facet |
Oliveira, Tales Lyra [UNIFESP] Candeia-Medeiros, Navylla Cavalcante-Araujo, Polliane M. Melo, Igor Santana Favaro-Pipi, Elaine Fatima, Luciana Alves [UNIFESP] Rocha, Antonio Augusto [UNIFESP] Goulart, Luiz Ricardo Machado, Ubiratan Fabres [UNIFESP] Campos, Ruy R. [UNIFESP] Sabino-Silva, Robinson |
author_role |
author |
author2 |
Candeia-Medeiros, Navylla Cavalcante-Araujo, Polliane M. Melo, Igor Santana Favaro-Pipi, Elaine Fatima, Luciana Alves [UNIFESP] Rocha, Antonio Augusto [UNIFESP] Goulart, Luiz Ricardo Machado, Ubiratan Fabres [UNIFESP] Campos, Ruy R. [UNIFESP] Sabino-Silva, Robinson |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Tales Lyra [UNIFESP] Candeia-Medeiros, Navylla Cavalcante-Araujo, Polliane M. Melo, Igor Santana Favaro-Pipi, Elaine Fatima, Luciana Alves [UNIFESP] Rocha, Antonio Augusto [UNIFESP] Goulart, Luiz Ricardo Machado, Ubiratan Fabres [UNIFESP] Campos, Ruy R. [UNIFESP] Sabino-Silva, Robinson |
description |
High glucose concentration in the airway surface liquid (ASL) is an important feature of diabetes that predisposes to respiratory infections. We investigated the role of alveolar epithelial SGLT1 activity on ASL glucose concentration and bacterial proliferation. Non-diabetic and diabetic rats were intranasally treated with saline, isoproterenol (to increase SGLT1 activity) or phlorizin (to decrease SGLT1 activity); 2 hours later, glucose concentration and bacterial proliferation (methicillin-resistant Sthaphylococcus aureus, MRSA and Pseudomonas aeruginosa, P. aeruginosa) were analyzed in bronchoalveolar lavage (BAL); and alveolar SGLT1 was analyzed by immunohistochemistry. BAL glucose concentration and bacterial proliferation increased in diabetic animals: isoproterenol stimulated SGLT1 migration to luminal membrane, and reduced (50%) the BAL glucose concentration; whereas phlorizin increased the BAL glucose concentration (100%). These regulations were accompanied by parallel changes of in vitro MRSA and P. aeruginosa proliferation in BAL (r = 0.9651 and r = 0.9613, respectively, Pearson correlation). The same regulations were observed in in vivo P. aeruginosa proliferation. In summary, the results indicate a relationship among SGLT1 activity, ASL glucose concentration and pulmonary bacterial proliferation. Besides, the study highlights that, in situations of pulmonary infection risk, such as in diabetic subjects, increased SGLT1 activity may prevent bacterial proliferation whereas decreased SGLT1 activity can exacerbate it. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-21T17:00:22Z |
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2020-08-21T17:00:22Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Scientific Reports. London, v. 6, p. -, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57962 http://dx.doi.org/10.1038/srep21752 |
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2045-2322 |
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WOS000370631800001.pdf |
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10.1038/srep21752 |
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WOS:000370631800001 |
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Scientific Reports. London, v. 6, p. -, 2016. 2045-2322 WOS000370631800001.pdf 10.1038/srep21752 WOS:000370631800001 |
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