Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/26050 https://dx.doi.org/10.1139/cjpp-77-4-286 |
Resumo: | The hypothalamic serotonergic system is involved in the regulation of food ingestion and energy metabolism. Since disturbances of both energy intake and expenditure can contribute to obesity, the objective of the present study was to evaluate the serotonergic response stimulated by food ingestion in two different models of obesity: the hyperphagic Zucker and the hypophagic and hypometabolic, monosodium glutamate (MSG) obese Wistar rat. for this we used microdialysis to examine the release of 5-hydroxytryptamine (serotonin, 5HT) and 5-hydroxyindoleacetic acid (5HIAA) in the lateral hypothalamus. Daily intake of MSG-obese rats was 40% lower while that of Zucker obese rats was 60% higher than that of the respective lean controls. in overnight-fasted animals, 20-min microdialysate samples were collected before (basal release) and during a 2-h period of access to a balanced palatable food mash. the animals began to eat during the first 20 min of food access, and food consumption was similar among the four groups in all six individual 20-min periods recorded. Ingestion of food increased 5HT release in all groups. in MSG-obese and lean Wistar rats, 5HT levels were similarly elevated during the whole experimental period. in the Zucker strain, 5HT increments of basal release tended to be higher in obese than in lean rats at 20 and 40 min, and a significantly higher increment was observed at 60 min after food access (40 and 135% for lean and obese, respectively). the area under the curve relating serotonin levels to the 120 min of food availability was significantly higher in Zucker obese (246.7 +/- 23.3) than MSG-obese (152.7 +/- 13.4), lean Wistar (151.9 +/- 11.1), and lean Zucker (173.5 +/- 24.0) rats. the present observation, of a food-induced serotonin release in the lateral hypothalamus of lean Wistar and Zucker rats, evidences that 5HT in the lateral hypothalamus is important in the normal response to feeding. in obese animals, the serotonin response was similar to (in the hypophagic-hypometabolic MSG model) or even higher than (in the hyperphagic Zucker model) that seen in the respective lean controls. This result indicates that the energy homeostasis disturbances of both these obesity models may not be ascribed to an impairment of the acute lateral hypothalamic serotonin response to a dietary stimulus. |
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Mori, Rosana Cristina Tieko [UNIFESP]Guimaraes, Regina Barros [UNIFESP]Nascimento, Claudia Maria da Penha Oller do [UNIFESP]Ribeiro, Eliane Beraldi [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:30:47Z2016-01-24T12:30:47Z1999-04-01Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada, v. 77, n. 4, p. 286-292, 1999.0008-4212https://repositorio.unifesp.br/handle/11600/26050https://dx.doi.org/10.1139/cjpp-77-4-28610.1139/cjpp-77-4-286WOS:000081297400007The hypothalamic serotonergic system is involved in the regulation of food ingestion and energy metabolism. Since disturbances of both energy intake and expenditure can contribute to obesity, the objective of the present study was to evaluate the serotonergic response stimulated by food ingestion in two different models of obesity: the hyperphagic Zucker and the hypophagic and hypometabolic, monosodium glutamate (MSG) obese Wistar rat. for this we used microdialysis to examine the release of 5-hydroxytryptamine (serotonin, 5HT) and 5-hydroxyindoleacetic acid (5HIAA) in the lateral hypothalamus. Daily intake of MSG-obese rats was 40% lower while that of Zucker obese rats was 60% higher than that of the respective lean controls. in overnight-fasted animals, 20-min microdialysate samples were collected before (basal release) and during a 2-h period of access to a balanced palatable food mash. the animals began to eat during the first 20 min of food access, and food consumption was similar among the four groups in all six individual 20-min periods recorded. Ingestion of food increased 5HT release in all groups. in MSG-obese and lean Wistar rats, 5HT levels were similarly elevated during the whole experimental period. in the Zucker strain, 5HT increments of basal release tended to be higher in obese than in lean rats at 20 and 40 min, and a significantly higher increment was observed at 60 min after food access (40 and 135% for lean and obese, respectively). the area under the curve relating serotonin levels to the 120 min of food availability was significantly higher in Zucker obese (246.7 +/- 23.3) than MSG-obese (152.7 +/- 13.4), lean Wistar (151.9 +/- 11.1), and lean Zucker (173.5 +/- 24.0) rats. the present observation, of a food-induced serotonin release in the lateral hypothalamus of lean Wistar and Zucker rats, evidences that 5HT in the lateral hypothalamus is important in the normal response to feeding. in obese animals, the serotonin response was similar to (in the hypophagic-hypometabolic MSG model) or even higher than (in the hyperphagic Zucker model) that seen in the respective lean controls. This result indicates that the energy homeostasis disturbances of both these obesity models may not be ascribed to an impairment of the acute lateral hypothalamic serotonin response to a dietary stimulus.Universidade Federal de São Paulo, Dept Physiol, Div Neurophysiol & Endocrin Physiol, BR-04023060 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, Div Neurophysiol & Endocrin Physiol, BR-04023060 São Paulo, BrazilWeb of Science286-292engNatl Research Council CanadaCanadian Journal of Physiology and PharmacologySerotoninFood intakeBrain microdialysisLateral hypothalamusMonosodium glutamate obesityZucker obesityLateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/260502021-09-30 17:25:19.359metadata only accessoai:repositorio.unifesp.br:11600/26050Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:15:50.305265Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
title |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
spellingShingle |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis Mori, Rosana Cristina Tieko [UNIFESP] Serotonin Food intake Brain microdialysis Lateral hypothalamus Monosodium glutamate obesity Zucker obesity |
title_short |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
title_full |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
title_fullStr |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
title_full_unstemmed |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
title_sort |
Lateral hypothalamic serotenergic responsiveness to food intake in rat obesity as measured by microdialysis |
author |
Mori, Rosana Cristina Tieko [UNIFESP] |
author_facet |
Mori, Rosana Cristina Tieko [UNIFESP] Guimaraes, Regina Barros [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
author_role |
author |
author2 |
Guimaraes, Regina Barros [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Mori, Rosana Cristina Tieko [UNIFESP] Guimaraes, Regina Barros [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] |
dc.subject.eng.fl_str_mv |
Serotonin Food intake Brain microdialysis Lateral hypothalamus Monosodium glutamate obesity Zucker obesity |
topic |
Serotonin Food intake Brain microdialysis Lateral hypothalamus Monosodium glutamate obesity Zucker obesity |
description |
The hypothalamic serotonergic system is involved in the regulation of food ingestion and energy metabolism. Since disturbances of both energy intake and expenditure can contribute to obesity, the objective of the present study was to evaluate the serotonergic response stimulated by food ingestion in two different models of obesity: the hyperphagic Zucker and the hypophagic and hypometabolic, monosodium glutamate (MSG) obese Wistar rat. for this we used microdialysis to examine the release of 5-hydroxytryptamine (serotonin, 5HT) and 5-hydroxyindoleacetic acid (5HIAA) in the lateral hypothalamus. Daily intake of MSG-obese rats was 40% lower while that of Zucker obese rats was 60% higher than that of the respective lean controls. in overnight-fasted animals, 20-min microdialysate samples were collected before (basal release) and during a 2-h period of access to a balanced palatable food mash. the animals began to eat during the first 20 min of food access, and food consumption was similar among the four groups in all six individual 20-min periods recorded. Ingestion of food increased 5HT release in all groups. in MSG-obese and lean Wistar rats, 5HT levels were similarly elevated during the whole experimental period. in the Zucker strain, 5HT increments of basal release tended to be higher in obese than in lean rats at 20 and 40 min, and a significantly higher increment was observed at 60 min after food access (40 and 135% for lean and obese, respectively). the area under the curve relating serotonin levels to the 120 min of food availability was significantly higher in Zucker obese (246.7 +/- 23.3) than MSG-obese (152.7 +/- 13.4), lean Wistar (151.9 +/- 11.1), and lean Zucker (173.5 +/- 24.0) rats. the present observation, of a food-induced serotonin release in the lateral hypothalamus of lean Wistar and Zucker rats, evidences that 5HT in the lateral hypothalamus is important in the normal response to feeding. in obese animals, the serotonin response was similar to (in the hypophagic-hypometabolic MSG model) or even higher than (in the hyperphagic Zucker model) that seen in the respective lean controls. This result indicates that the energy homeostasis disturbances of both these obesity models may not be ascribed to an impairment of the acute lateral hypothalamic serotonin response to a dietary stimulus. |
publishDate |
1999 |
dc.date.issued.fl_str_mv |
1999-04-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:30:47Z |
dc.date.available.fl_str_mv |
2016-01-24T12:30:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada, v. 77, n. 4, p. 286-292, 1999. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/26050 https://dx.doi.org/10.1139/cjpp-77-4-286 |
dc.identifier.issn.none.fl_str_mv |
0008-4212 |
dc.identifier.doi.none.fl_str_mv |
10.1139/cjpp-77-4-286 |
dc.identifier.wos.none.fl_str_mv |
WOS:000081297400007 |
identifier_str_mv |
Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada, v. 77, n. 4, p. 286-292, 1999. 0008-4212 10.1139/cjpp-77-4-286 WOS:000081297400007 |
url |
https://repositorio.unifesp.br/handle/11600/26050 https://dx.doi.org/10.1139/cjpp-77-4-286 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Canadian Journal of Physiology and Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
286-292 |
dc.publisher.none.fl_str_mv |
Natl Research Council Canada |
publisher.none.fl_str_mv |
Natl Research Council Canada |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460269428572160 |