Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors

Detalhes bibliográficos
Autor(a) principal: Salgado, Flavio Luiz Lima [UNIFESP]
Data de Publicação: 2016
Outros Autores: Artigiani-Neto, Ricardo [UNIFESP], Lopes Filho, Gaspar de Jesus [UNIFESP]
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/0102-6720201600040003
https://repositorio.unifesp.br/handle/11600/56938
Resumo: Background: Healing is an innate biological phenomenon, and carcinogenesis acquired, but with common humoral and cellular elements. Carcinogenesis interferes negatively in healing. Aim: To evaluate the histological changes in laparotomy scars of healthy Balb/c mice and with an Ehrlich tumor in its various forms of presentation. Methods: Fifty-four mice were divided into three groups of 18 animals. First group was the control
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spelling Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich TumorsAvaliação dos fatores de crescimento e cox-2 na cicatrização de camundongos com neoplasia de EhrlichCarcinomaEhrlich TumorWound healingCyclooxygenase 2Vascular endothelial growth factor receptor-1Fibroblast growth factor 2CarcinomaTumor de EhrlichCicatrizaçãoCyclo-oxygenase 2Fator de crescimento do endotélio vascularFator de crescimento dos fibroblastos 2Receptor-1Background: Healing is an innate biological phenomenon, and carcinogenesis acquired, but with common humoral and cellular elements. Carcinogenesis interferes negatively in healing. Aim: To evaluate the histological changes in laparotomy scars of healthy Balb/c mice and with an Ehrlich tumor in its various forms of presentation. Methods: Fifty-four mice were divided into three groups of 18 animals. First group was the controlthe second had Ehrlich tumor with ascitesand the third had the subcutaneous form of this tumor. Seven days after tumor inoculation, all 54 mice were submitted to laparotomy. All of the animals in the experiment were operated on again on 7th day after surgery, with resection of the scar and subsequent euthanasia of the animal. The scars were sent for histological assessment using immunohistochemical techniques to evaluate Cox-2 (cyclooxygenase 2), VEGF (vascular endothelial growth factor) and FGF (fibroblast growth factor). Semi-quantitatively analysis was done in the laparotomy scars and in the abdominal walls far away from the site of the operation. Results: Assessing the weight of the animals, the correct inoculation of the tumor and weight gain in the group with tumoral ascites was observed. The histological studies showed that groups with the tumor showed a statistically significant higher presence of Cox-2 compared to the control. In the Cox-2 analysis of the abdominal wall, the ascites group showed the most significant difference. VEGF did not present any significant differences between the three groups, regardless of the site. The FGF showed a significant increase in animals with the tumor. Conclusion: Histological findings in both laparotomy scar and the abdominal wall showed that with Ehrlich's neoplasia there was an exacerbated inflammatory response, translated by more intense expression of Cox-2 and greater fibroblast proliferation, translated by more intense expression of FGF, that is, it stimulated both the immediate inflammatory reactions, observed with Cox-2 reactions, and late scarring by fibroblasts and FGF.Racional: A cicatrização é fenômeno biológico inato, e a carcinogênese adquirido, mas com elementos humorais e celulares comuns. A carcinogênese interfere de forma negativa na cicatrização. Objetivo: Avaliar as modificações histológicas nas cicatrizes laparotômicas de camundongos Balb/c sadios como controles, e com a neoplasia de Ehrlich, em suas diferentes formas de apresentação. Métodos: Foram utilizados 54 camundongos, divididos em três grupos de 18 animais cada um. O primeiro era controle; o segundo com a neoplasia de Ehrlich em sua forma ascítica; e o terceiro na forma subcutânea. Sete dias após a inoculação do tumor, todos os 54 camundongos foram submetidos à laparotomia e reoperados no sétimo dia de pós-operatório, com ressecção da cicatriz e posterior eutanásia. As cicatrizes foram encaminhadas para estudo histológico com técnicas imunoistoquímicas para avaliar Cox-2 (ciclo-oxigenase 2), VEGF (fator de crescimento do endotélio vascular) e FGF (fator de crescimento dos fibroblastos) e analisadas de forma semiquantitativana tanto na cicatriz laparotômica como na parede abdominal mais distante do local operado. Resultados: Avaliando o peso, observou-se a correta inoculação do tumor e o aumento de peso no grupo com a neoplasia na modalidade ascítica. Os estudos histológicos mostraram que os grupos com a neoplasia apresentaram maior presença da Cox-2 em relação ao controle, estatisticamente significante. No estudo da Cox-2 da parede abdominal foi o local em que o grupo ascítico apresentou a diferença mais expressiva. O VEGF não apresentou diferenças significantes entre os três grupos, independentemente do local estudado. O FGF teve aumento significante nos animais com neoplasia. Conclusão: Os achados histológicos encontrados tanto na cicatriz das laparotomias quanto na parede abdominal mostraram que com a neoplasia de Ehrlich houve resposta inflamatória exacerbada, traduzida por expressão mais intensa da Cox-2 e maior proliferação fibroblástica, traduzida por expressão mais intensa do FGF, ou seja, estimulou tanto as reações inflamatórias imediatas, observadas nas reações da Cox-2, como nas cicatriciais tardias com os fibroblastos e o FGF.Fed Univ Sao Paulo UNIFESP, Paulista Sch Med, Postgrad Program Interdisciplinary Surg Sci, Sao Paulo, SP, BrazilPrograma de Pós-graduação em Ciência Cirúrgica Interdisciplinar, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, São Paulo, SP, BrasilWeb of ScienceFundo Nacional de Saude - Sistema Nacional de Transplante (SNT)Colegio Brasileiro Cirurgia Digestiva-Cbcd2020-07-31T12:47:37Z2020-07-31T12:47:37Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion223-226application/pdfapplication/pdfhttp://dx.doi.org/10.1590/0102-6720201600040003Abcd-Arquivos Brasileiros De Cirurgia Digestiva-Brazilian Archives Of Digestive Surgery. Sao Paulo Sp, v. 29, n. 4, p. 223-226, 2016.10.1590/0102-6720201600040003S0102-67202016000400223-en.pdfS0102-67202016000400223-pt.pdf0102-6720S0102-67202016000400223https://repositorio.unifesp.br/handle/11600/56938WOS:000392703900003porengAbcd-Arquivos Brasileiros De Cirurgia Digestiva-Brazilian Archives Of Digestive SurgerySao Paulo Spinfo:eu-repo/semantics/openAccessSalgado, Flavio Luiz Lima [UNIFESP]Artigiani-Neto, Ricardo [UNIFESP]Lopes Filho, Gaspar de Jesus [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-11T17:04:19Zoai:repositorio.unifesp.br/:11600/56938Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-11T17:04:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
Avaliação dos fatores de crescimento e cox-2 na cicatrização de camundongos com neoplasia de Ehrlich
title Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
spellingShingle Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
Salgado, Flavio Luiz Lima [UNIFESP]
Carcinoma
Ehrlich Tumor
Wound healing
Cyclooxygenase 2
Vascular endothelial growth factor receptor-1
Fibroblast growth factor 2
Carcinoma
Tumor de Ehrlich
Cicatrização
Cyclo-oxygenase 2
Fator de crescimento do endotélio vascular
Fator de crescimento dos fibroblastos 2
Receptor-1
title_short Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
title_full Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
title_fullStr Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
title_full_unstemmed Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
title_sort Growth Factors And Cox2 In Wound Healing: An Experimental Study With Ehrlich Tumors
author Salgado, Flavio Luiz Lima [UNIFESP]
author_facet Salgado, Flavio Luiz Lima [UNIFESP]
Artigiani-Neto, Ricardo [UNIFESP]
Lopes Filho, Gaspar de Jesus [UNIFESP]
author_role author
author2 Artigiani-Neto, Ricardo [UNIFESP]
Lopes Filho, Gaspar de Jesus [UNIFESP]
author2_role author
author
dc.contributor.author.fl_str_mv Salgado, Flavio Luiz Lima [UNIFESP]
Artigiani-Neto, Ricardo [UNIFESP]
Lopes Filho, Gaspar de Jesus [UNIFESP]
dc.subject.por.fl_str_mv Carcinoma
Ehrlich Tumor
Wound healing
Cyclooxygenase 2
Vascular endothelial growth factor receptor-1
Fibroblast growth factor 2
Carcinoma
Tumor de Ehrlich
Cicatrização
Cyclo-oxygenase 2
Fator de crescimento do endotélio vascular
Fator de crescimento dos fibroblastos 2
Receptor-1
topic Carcinoma
Ehrlich Tumor
Wound healing
Cyclooxygenase 2
Vascular endothelial growth factor receptor-1
Fibroblast growth factor 2
Carcinoma
Tumor de Ehrlich
Cicatrização
Cyclo-oxygenase 2
Fator de crescimento do endotélio vascular
Fator de crescimento dos fibroblastos 2
Receptor-1
description Background: Healing is an innate biological phenomenon, and carcinogenesis acquired, but with common humoral and cellular elements. Carcinogenesis interferes negatively in healing. Aim: To evaluate the histological changes in laparotomy scars of healthy Balb/c mice and with an Ehrlich tumor in its various forms of presentation. Methods: Fifty-four mice were divided into three groups of 18 animals. First group was the control
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-07-31T12:47:37Z
2020-07-31T12:47:37Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0102-6720201600040003
Abcd-Arquivos Brasileiros De Cirurgia Digestiva-Brazilian Archives Of Digestive Surgery. Sao Paulo Sp, v. 29, n. 4, p. 223-226, 2016.
10.1590/0102-6720201600040003
S0102-67202016000400223-en.pdf
S0102-67202016000400223-pt.pdf
0102-6720
S0102-67202016000400223
https://repositorio.unifesp.br/handle/11600/56938
WOS:000392703900003
url http://dx.doi.org/10.1590/0102-6720201600040003
https://repositorio.unifesp.br/handle/11600/56938
identifier_str_mv Abcd-Arquivos Brasileiros De Cirurgia Digestiva-Brazilian Archives Of Digestive Surgery. Sao Paulo Sp, v. 29, n. 4, p. 223-226, 2016.
10.1590/0102-6720201600040003
S0102-67202016000400223-en.pdf
S0102-67202016000400223-pt.pdf
0102-6720
S0102-67202016000400223
WOS:000392703900003
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv Abcd-Arquivos Brasileiros De Cirurgia Digestiva-Brazilian Archives Of Digestive Surgery
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 223-226
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Sao Paulo Sp
dc.publisher.none.fl_str_mv Colegio Brasileiro Cirurgia Digestiva-Cbcd
publisher.none.fl_str_mv Colegio Brasileiro Cirurgia Digestiva-Cbcd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268432071262208

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