Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model

Detalhes bibliográficos
Autor(a) principal: Calió, Michele Longoni [UNIFESP]
Data de Publicação: 2014
Outros Autores: Marinho, Darci Sousa [UNIFESP], Ko, Gui Mi [UNIFESP], Ribeiro, Renata Rodrigues [UNIFESP], Carbonel, Adriana Ferraz [UNIFESP], Oyama, Lila Missae [UNIFESP], Ormanji, Milene Subtil [UNIFESP], Guirao, Tatiana Pinoti [UNIFESP], Calió, Pedro Luiz, Reis, Luciana Aparecida [UNIFESP], Simões, Manuel de Jesus [UNIFESP], Lisboa-Nascimento, Telma [UNIFESP], Ferreira, Alice Teixeira [UNIFESP], Bertoncini, Clelia Rejane Antonio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.freeradbiomed.2014.01.024
http://repositorio.unifesp.br/handle/11600/37682
Resumo: Stroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. the aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. the results of qRT-PCR assays showed higher levels of the antiapoptotic BcI-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. in addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats. (c) 2014 the Authors. Published by Elsevier Inc.
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spelling Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke modelMesenchymal stem cellsStrokeApoptosisSuperoxideLipid peroxidationFree radicalsStroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. the aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. the results of qRT-PCR assays showed higher levels of the antiapoptotic BcI-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. in addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats. (c) 2014 the Authors. Published by Elsevier Inc.Universidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Desenvolvimento Modelos Expt Med & Biol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniv Santa Cecilia, Dept Odontol, Santos, SP, BrazilUniversidade Federal de São Paulo, Dept Nefrol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Desenvolvimento Modelos Expt Med & Biol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Nefrol, BR-04023062 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 05/60630-1FAPESP: 10/00106-5Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Univ Santa CeciliaCalió, Michele Longoni [UNIFESP]Marinho, Darci Sousa [UNIFESP]Ko, Gui Mi [UNIFESP]Ribeiro, Renata Rodrigues [UNIFESP]Carbonel, Adriana Ferraz [UNIFESP]Oyama, Lila Missae [UNIFESP]Ormanji, Milene Subtil [UNIFESP]Guirao, Tatiana Pinoti [UNIFESP]Calió, Pedro LuizReis, Luciana Aparecida [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Lisboa-Nascimento, Telma [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Bertoncini, Clelia Rejane Antonio [UNIFESP]2016-01-24T14:37:08Z2016-01-24T14:37:08Z2014-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion141-154application/pdfhttp://dx.doi.org/10.1016/j.freeradbiomed.2014.01.024Free Radical Biology and Medicine. New York: Elsevier B.V., v. 70, p. 141-154, 2014.10.1016/j.freeradbiomed.2014.01.024WOS000335487100014.pdf0891-5849http://repositorio.unifesp.br/handle/11600/37682WOS:000335487100014engFree Radical Biology and Medicineinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T21:57:23Zoai:repositorio.unifesp.br/:11600/37682Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T21:57:23Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
title Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
spellingShingle Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
Calió, Michele Longoni [UNIFESP]
Mesenchymal stem cells
Stroke
Apoptosis
Superoxide
Lipid peroxidation
Free radicals
title_short Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
title_full Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
title_fullStr Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
title_full_unstemmed Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
title_sort Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model
author Calió, Michele Longoni [UNIFESP]
author_facet Calió, Michele Longoni [UNIFESP]
Marinho, Darci Sousa [UNIFESP]
Ko, Gui Mi [UNIFESP]
Ribeiro, Renata Rodrigues [UNIFESP]
Carbonel, Adriana Ferraz [UNIFESP]
Oyama, Lila Missae [UNIFESP]
Ormanji, Milene Subtil [UNIFESP]
Guirao, Tatiana Pinoti [UNIFESP]
Calió, Pedro Luiz
Reis, Luciana Aparecida [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Lisboa-Nascimento, Telma [UNIFESP]
Ferreira, Alice Teixeira [UNIFESP]
Bertoncini, Clelia Rejane Antonio [UNIFESP]
author_role author
author2 Marinho, Darci Sousa [UNIFESP]
Ko, Gui Mi [UNIFESP]
Ribeiro, Renata Rodrigues [UNIFESP]
Carbonel, Adriana Ferraz [UNIFESP]
Oyama, Lila Missae [UNIFESP]
Ormanji, Milene Subtil [UNIFESP]
Guirao, Tatiana Pinoti [UNIFESP]
Calió, Pedro Luiz
Reis, Luciana Aparecida [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Lisboa-Nascimento, Telma [UNIFESP]
Ferreira, Alice Teixeira [UNIFESP]
Bertoncini, Clelia Rejane Antonio [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Univ Santa Cecilia
dc.contributor.author.fl_str_mv Calió, Michele Longoni [UNIFESP]
Marinho, Darci Sousa [UNIFESP]
Ko, Gui Mi [UNIFESP]
Ribeiro, Renata Rodrigues [UNIFESP]
Carbonel, Adriana Ferraz [UNIFESP]
Oyama, Lila Missae [UNIFESP]
Ormanji, Milene Subtil [UNIFESP]
Guirao, Tatiana Pinoti [UNIFESP]
Calió, Pedro Luiz
Reis, Luciana Aparecida [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Lisboa-Nascimento, Telma [UNIFESP]
Ferreira, Alice Teixeira [UNIFESP]
Bertoncini, Clelia Rejane Antonio [UNIFESP]
dc.subject.por.fl_str_mv Mesenchymal stem cells
Stroke
Apoptosis
Superoxide
Lipid peroxidation
Free radicals
topic Mesenchymal stem cells
Stroke
Apoptosis
Superoxide
Lipid peroxidation
Free radicals
description Stroke is the most common cause of motor disabilities and is a major cause of mortality worldwide. Adult stem cells have been shown to be effective against neuronal degeneration through mechanisms that include both the recovery of neurotransmitter activity and a decrease in apoptosis and oxidative stress. We chose the lineage stroke-prone spontaneously hypertensive rat (SHRSP) as a model for stem cell therapy. SHRSP rats can develop such severe hypertension that they generally suffer a stroke at approximately 1 year of age. the aim of this study was to evaluate whether mesenchymal stem cells (MSCs) decrease apoptotic death and oxidative stress in existing SHRSP brain tissue. the results of qRT-PCR assays showed higher levels of the antiapoptotic BcI-2 gene in the MSC-treated animals, compared with untreated. Our study also showed that superoxide, apoptotic cells, and by-products of lipid peroxidation decreased in MSC-treated SHRSP to levels similar those found in the animal controls, Wistar Kyoto rats. in addition, we saw a repair of morphological damage at the hippocampal region after MSC transplantation. These data suggest that MSCs have neuroprotective and antioxidant potential in stroke-prone spontaneously hypertensive rats. (c) 2014 the Authors. Published by Elsevier Inc.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-01
2016-01-24T14:37:08Z
2016-01-24T14:37:08Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.freeradbiomed.2014.01.024
Free Radical Biology and Medicine. New York: Elsevier B.V., v. 70, p. 141-154, 2014.
10.1016/j.freeradbiomed.2014.01.024
WOS000335487100014.pdf
0891-5849
http://repositorio.unifesp.br/handle/11600/37682
WOS:000335487100014
url http://dx.doi.org/10.1016/j.freeradbiomed.2014.01.024
http://repositorio.unifesp.br/handle/11600/37682
identifier_str_mv Free Radical Biology and Medicine. New York: Elsevier B.V., v. 70, p. 141-154, 2014.
10.1016/j.freeradbiomed.2014.01.024
WOS000335487100014.pdf
0891-5849
WOS:000335487100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Free Radical Biology and Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 141-154
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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