Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains

Detalhes bibliográficos
Autor(a) principal: Hernandes, Rodrigo T. [UNIFESP]
Data de Publicação: 2011
Outros Autores: Velsko, Irina, Sampaio, Suely C. F. [UNIFESP], Elias Junior, Waldir Pereira [UNIFESP], Robins-Browne, Roy M., Gomes, Tania A. T. [UNIFESP], Giron, Jorge A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1128/AEM.05376-11
http://repositorio.unifesp.br/handle/11600/34255
Resumo: Atypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host.
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spelling Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli StrainsAtypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host.Univ Florida, Dept Mol Genet & Microbiol, Emerging Pathol Inst, Gainesville, FL 32610 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilInst Butantan, Bacteriol Lab, São Paulo, BrazilUniv Melbourne, Dept Microbiol & Immunol, Melbourne, Vic 3010, AustraliaRoyal Childrens Hosp, Murdoch Children Res Inst, Melbourne, Vic 3010, AustraliaUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceNIAID, NIHFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Australian National Health and Medical Research CouncilNIAID, NIH: AI66012FAPESP: 06/54359-6CNPq: 510178/2010-3Amer Soc MicrobiologyUniv FloridaUniversidade Federal de São Paulo (UNIFESP)Inst ButantanUniv MelbourneRoyal Childrens HospHernandes, Rodrigo T. [UNIFESP]Velsko, IrinaSampaio, Suely C. F. [UNIFESP]Elias Junior, Waldir Pereira [UNIFESP]Robins-Browne, Roy M.Gomes, Tania A. T. [UNIFESP]Giron, Jorge A.2016-01-24T14:17:29Z2016-01-24T14:17:29Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8391-8399application/pdfhttp://dx.doi.org/10.1128/AEM.05376-11Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011.10.1128/AEM.05376-11WOS000297164100025.pdf0099-2240http://repositorio.unifesp.br/handle/11600/34255WOS:000297164100025engApplied and Environmental Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T09:37:17Zoai:repositorio.unifesp.br/:11600/34255Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T09:37:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
title Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
spellingShingle Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
Hernandes, Rodrigo T. [UNIFESP]
title_short Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
title_full Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
title_fullStr Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
title_full_unstemmed Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
title_sort Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
author Hernandes, Rodrigo T. [UNIFESP]
author_facet Hernandes, Rodrigo T. [UNIFESP]
Velsko, Irina
Sampaio, Suely C. F. [UNIFESP]
Elias Junior, Waldir Pereira [UNIFESP]
Robins-Browne, Roy M.
Gomes, Tania A. T. [UNIFESP]
Giron, Jorge A.
author_role author
author2 Velsko, Irina
Sampaio, Suely C. F. [UNIFESP]
Elias Junior, Waldir Pereira [UNIFESP]
Robins-Browne, Roy M.
Gomes, Tania A. T. [UNIFESP]
Giron, Jorge A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Florida
Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Univ Melbourne
Royal Childrens Hosp
dc.contributor.author.fl_str_mv Hernandes, Rodrigo T. [UNIFESP]
Velsko, Irina
Sampaio, Suely C. F. [UNIFESP]
Elias Junior, Waldir Pereira [UNIFESP]
Robins-Browne, Roy M.
Gomes, Tania A. T. [UNIFESP]
Giron, Jorge A.
description Atypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2016-01-24T14:17:29Z
2016-01-24T14:17:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1128/AEM.05376-11
Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011.
10.1128/AEM.05376-11
WOS000297164100025.pdf
0099-2240
http://repositorio.unifesp.br/handle/11600/34255
WOS:000297164100025
url http://dx.doi.org/10.1128/AEM.05376-11
http://repositorio.unifesp.br/handle/11600/34255
identifier_str_mv Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011.
10.1128/AEM.05376-11
WOS000297164100025.pdf
0099-2240
WOS:000297164100025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied and Environmental Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8391-8399
application/pdf
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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