Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1128/AEM.05376-11 http://repositorio.unifesp.br/handle/11600/34255 |
Resumo: | Atypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host. |
id |
UFSP_96de63486b85d2acd06af8685635912e |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/34255 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli StrainsAtypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host.Univ Florida, Dept Mol Genet & Microbiol, Emerging Pathol Inst, Gainesville, FL 32610 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilInst Butantan, Bacteriol Lab, São Paulo, BrazilUniv Melbourne, Dept Microbiol & Immunol, Melbourne, Vic 3010, AustraliaRoyal Childrens Hosp, Murdoch Children Res Inst, Melbourne, Vic 3010, AustraliaUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceNIAID, NIHFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Australian National Health and Medical Research CouncilNIAID, NIH: AI66012FAPESP: 06/54359-6CNPq: 510178/2010-3Amer Soc MicrobiologyUniv FloridaUniversidade Federal de São Paulo (UNIFESP)Inst ButantanUniv MelbourneRoyal Childrens HospHernandes, Rodrigo T. [UNIFESP]Velsko, IrinaSampaio, Suely C. F. [UNIFESP]Elias Junior, Waldir Pereira [UNIFESP]Robins-Browne, Roy M.Gomes, Tania A. T. [UNIFESP]Giron, Jorge A.2016-01-24T14:17:29Z2016-01-24T14:17:29Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8391-8399application/pdfhttp://dx.doi.org/10.1128/AEM.05376-11Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011.10.1128/AEM.05376-11WOS000297164100025.pdf0099-2240http://repositorio.unifesp.br/handle/11600/34255WOS:000297164100025engApplied and Environmental Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T09:37:17Zoai:repositorio.unifesp.br/:11600/34255Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T09:37:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
title |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
spellingShingle |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains Hernandes, Rodrigo T. [UNIFESP] |
title_short |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
title_full |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
title_fullStr |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
title_full_unstemmed |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
title_sort |
Fimbrial Adhesins Produced by Atypical Enteropathogenic Escherichia coli Strains |
author |
Hernandes, Rodrigo T. [UNIFESP] |
author_facet |
Hernandes, Rodrigo T. [UNIFESP] Velsko, Irina Sampaio, Suely C. F. [UNIFESP] Elias Junior, Waldir Pereira [UNIFESP] Robins-Browne, Roy M. Gomes, Tania A. T. [UNIFESP] Giron, Jorge A. |
author_role |
author |
author2 |
Velsko, Irina Sampaio, Suely C. F. [UNIFESP] Elias Junior, Waldir Pereira [UNIFESP] Robins-Browne, Roy M. Gomes, Tania A. T. [UNIFESP] Giron, Jorge A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Florida Universidade Federal de São Paulo (UNIFESP) Inst Butantan Univ Melbourne Royal Childrens Hosp |
dc.contributor.author.fl_str_mv |
Hernandes, Rodrigo T. [UNIFESP] Velsko, Irina Sampaio, Suely C. F. [UNIFESP] Elias Junior, Waldir Pereira [UNIFESP] Robins-Browne, Roy M. Gomes, Tania A. T. [UNIFESP] Giron, Jorge A. |
description |
Atypical enteropathogenic Escherichia coli (aEPEC) has emerged as a significant cause of pediatric diarrhea worldwide; however, information regarding its adherence mechanisms to the human gut mucosa is lacking. in this study, we investigated the prevalence of several (fimA, ecpA, csgA, elfA, and hcpA) fimbrial genes in 71 aEPEC strains isolated from children with diarrhea (54 strains) and healthy individuals (17 strains) in Brazil and Australia by PCR. These genes are associated with adhesion and/or biofilm formation of pathogenic and commensal E. coli. Here, the most prevalent fimbrial genes found, in descending order, were hcpA (98.6%), ecpA (86%), fimA (76%), elfA (72%), and csgA (19.7%). Phenotypic expression of pili in aEPEC strains was assessed by several approaches. We were not able to detect the hemorrhagic coli pilus (HCP) or the E. coli laminin-binding fimbriae (ELF) in these strains by using immunofluorescence. Type 1 pili and curli were detected in 59% (by yeast agglutination) and 2.8% (by Congo red binding and immunofluorescence) of the strains, respectively. the E. coli common pilus (ECP) was evidenced in 36.6% of the strains on bacteria adhering to HeLa cells by immunofluorescence, suggesting that ECP could play an important role in cell adherence for some aEPEC strains. This study highlights the complex nature of the adherence mechanisms of aEPEC strains involving the coordinated function of fimbrial (e.g., ECP) and nonfimbrial (e.g., intimin) adhesins and indicates that these strains bear several pilus operons that could potentially be expressed in different niches favoring colonization and survival in and outside the host. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 2016-01-24T14:17:29Z 2016-01-24T14:17:29Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/AEM.05376-11 Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011. 10.1128/AEM.05376-11 WOS000297164100025.pdf 0099-2240 http://repositorio.unifesp.br/handle/11600/34255 WOS:000297164100025 |
url |
http://dx.doi.org/10.1128/AEM.05376-11 http://repositorio.unifesp.br/handle/11600/34255 |
identifier_str_mv |
Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 77, n. 23, p. 8391-8399, 2011. 10.1128/AEM.05376-11 WOS000297164100025.pdf 0099-2240 WOS:000297164100025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied and Environmental Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8391-8399 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268458082238464 |