Estudo da regeneração esplênica autóloga e heterotópica em ratos
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502005000300011 http://repositorio.unifesp.br/handle/11600/2583 |
Resumo: | PURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen. |
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Estudo da regeneração esplênica autóloga e heterotópica em ratosAntologus and heterotopic splenic regeneration in ratsSpleenTransplantSplenosisWistar ratsBaçoTransplante esplênicoEsplenosePURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen.OBJETIVO: estudar a regeneração esplênica dos fragmentos de baço autólogos e heterotópicos implantados na cavidade peritoneal e na tela subcutânea em ratos e comparar os seus aspectos histológicos e citológicos com baço normal. MÉTODOS: 44 ratos Wistar foram submetidos a esplenectomia e receberam fragmentos de auto-implante: GA = na tela subcutânea (n=22) e GB na cavidade peritoneal (n=22). GAI (n=11) e GAII (n=11) receberam implante de fragmento único. GBIII (n=11) e GBIV (n=11) receberam implantes de quatro fragmentos. O baço remanescente foi usado como controle. Após 5 semanas de foram submetidos à eutanásia e foram investigados os aspectos macroscópicos e microscópicos (histológicos e citológicos) dos fragmentos nos seus locais de implante. Os resultados foram submetidos a testes estatísticos não paramétricos(p>0,05). RESULTADOS: Não houve diferença estatística significante entre o grupo que recebeu implante na tela subcutânea (n=22) e o grupo da cavidade peritoneal (n=22). Quanto à presença ou ausência de tecidos esplênicos regenerados (p=0,182), também não apresentou diferença com significância estatística. Os estudos histológicos e citológicos do tecido regenerado não evidenciaram diferenças em relação ao grupo controle. CONCLUSÃO: A regeneração do tecido esplênico autólogo e heterotópico em ratos ocorre nas mesmas proporções tanto na tela subcutânea como na cavidade peritoneal. Os aspectos histológicos e citológicos são semelhantes aos do baço normal.Santa Casa de São Paulo Faculdade de MedicinaUniversidade Estadual de LondrinaUNIFESP-EPMUNIFESP, EPMSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaSanta Casa de São Paulo Faculdade de MedicinaUniversidade Estadual de LondrinaUniversidade Federal de São Paulo (UNIFESP)Souza, José Carlos Lacerda DeAthiê, EmilioMarigo, CarlosRahal, FaresFagundes, Djalma José [UNIFESP]2015-06-14T13:31:39Z2015-06-14T13:31:39Z2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion253-257application/pdfhttp://dx.doi.org/10.1590/S0102-86502005000300011Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005.10.1590/S0102-86502005000300011S0102-86502005000300011.pdf0102-8650S0102-86502005000300011http://repositorio.unifesp.br/handle/11600/2583porActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T09:02:26Zoai:repositorio.unifesp.br/:11600/2583Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T09:02:26Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Estudo da regeneração esplênica autóloga e heterotópica em ratos Antologus and heterotopic splenic regeneration in rats |
title |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
spellingShingle |
Estudo da regeneração esplênica autóloga e heterotópica em ratos Souza, José Carlos Lacerda De Spleen Transplant Splenosis Wistar rats Baço Transplante esplênico Esplenose |
title_short |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
title_full |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
title_fullStr |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
title_full_unstemmed |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
title_sort |
Estudo da regeneração esplênica autóloga e heterotópica em ratos |
author |
Souza, José Carlos Lacerda De |
author_facet |
Souza, José Carlos Lacerda De Athiê, Emilio Marigo, Carlos Rahal, Fares Fagundes, Djalma José [UNIFESP] |
author_role |
author |
author2 |
Athiê, Emilio Marigo, Carlos Rahal, Fares Fagundes, Djalma José [UNIFESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Santa Casa de São Paulo Faculdade de Medicina Universidade Estadual de Londrina Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Souza, José Carlos Lacerda De Athiê, Emilio Marigo, Carlos Rahal, Fares Fagundes, Djalma José [UNIFESP] |
dc.subject.por.fl_str_mv |
Spleen Transplant Splenosis Wistar rats Baço Transplante esplênico Esplenose |
topic |
Spleen Transplant Splenosis Wistar rats Baço Transplante esplênico Esplenose |
description |
PURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 2015-06-14T13:31:39Z 2015-06-14T13:31:39Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502005000300011 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005. 10.1590/S0102-86502005000300011 S0102-86502005000300011.pdf 0102-8650 S0102-86502005000300011 http://repositorio.unifesp.br/handle/11600/2583 |
url |
http://dx.doi.org/10.1590/S0102-86502005000300011 http://repositorio.unifesp.br/handle/11600/2583 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005. 10.1590/S0102-86502005000300011 S0102-86502005000300011.pdf 0102-8650 S0102-86502005000300011 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
253-257 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268315036549120 |