Estudo da regeneração esplênica autóloga e heterotópica em ratos

Detalhes bibliográficos
Autor(a) principal: Souza, José Carlos Lacerda De
Data de Publicação: 2005
Outros Autores: Athiê, Emilio, Marigo, Carlos, Rahal, Fares, Fagundes, Djalma José [UNIFESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502005000300011
http://repositorio.unifesp.br/handle/11600/2583
Resumo: PURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen.
id UFSP_9824ad107214fb1cd9adf018fca905d9
oai_identifier_str oai:repositorio.unifesp.br/:11600/2583
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Estudo da regeneração esplênica autóloga e heterotópica em ratosAntologus and heterotopic splenic regeneration in ratsSpleenTransplantSplenosisWistar ratsBaçoTransplante esplênicoEsplenosePURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen.OBJETIVO: estudar a regeneração esplênica dos fragmentos de baço autólogos e heterotópicos implantados na cavidade peritoneal e na tela subcutânea em ratos e comparar os seus aspectos histológicos e citológicos com baço normal. MÉTODOS: 44 ratos Wistar foram submetidos a esplenectomia e receberam fragmentos de auto-implante: GA = na tela subcutânea (n=22) e GB na cavidade peritoneal (n=22). GAI (n=11) e GAII (n=11) receberam implante de fragmento único. GBIII (n=11) e GBIV (n=11) receberam implantes de quatro fragmentos. O baço remanescente foi usado como controle. Após 5 semanas de foram submetidos à eutanásia e foram investigados os aspectos macroscópicos e microscópicos (histológicos e citológicos) dos fragmentos nos seus locais de implante. Os resultados foram submetidos a testes estatísticos não paramétricos(p>0,05). RESULTADOS: Não houve diferença estatística significante entre o grupo que recebeu implante na tela subcutânea (n=22) e o grupo da cavidade peritoneal (n=22). Quanto à presença ou ausência de tecidos esplênicos regenerados (p=0,182), também não apresentou diferença com significância estatística. Os estudos histológicos e citológicos do tecido regenerado não evidenciaram diferenças em relação ao grupo controle. CONCLUSÃO: A regeneração do tecido esplênico autólogo e heterotópico em ratos ocorre nas mesmas proporções tanto na tela subcutânea como na cavidade peritoneal. Os aspectos histológicos e citológicos são semelhantes aos do baço normal.Santa Casa de São Paulo Faculdade de MedicinaUniversidade Estadual de LondrinaUNIFESP-EPMUNIFESP, EPMSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaSanta Casa de São Paulo Faculdade de MedicinaUniversidade Estadual de LondrinaUniversidade Federal de São Paulo (UNIFESP)Souza, José Carlos Lacerda DeAthiê, EmilioMarigo, CarlosRahal, FaresFagundes, Djalma José [UNIFESP]2015-06-14T13:31:39Z2015-06-14T13:31:39Z2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion253-257application/pdfhttp://dx.doi.org/10.1590/S0102-86502005000300011Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005.10.1590/S0102-86502005000300011S0102-86502005000300011.pdf0102-8650S0102-86502005000300011http://repositorio.unifesp.br/handle/11600/2583porActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T09:02:26Zoai:repositorio.unifesp.br/:11600/2583Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T09:02:26Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Estudo da regeneração esplênica autóloga e heterotópica em ratos
Antologus and heterotopic splenic regeneration in rats
title Estudo da regeneração esplênica autóloga e heterotópica em ratos
spellingShingle Estudo da regeneração esplênica autóloga e heterotópica em ratos
Souza, José Carlos Lacerda De
Spleen
Transplant
Splenosis
Wistar rats
Baço
Transplante esplênico
Esplenose
title_short Estudo da regeneração esplênica autóloga e heterotópica em ratos
title_full Estudo da regeneração esplênica autóloga e heterotópica em ratos
title_fullStr Estudo da regeneração esplênica autóloga e heterotópica em ratos
title_full_unstemmed Estudo da regeneração esplênica autóloga e heterotópica em ratos
title_sort Estudo da regeneração esplênica autóloga e heterotópica em ratos
author Souza, José Carlos Lacerda De
author_facet Souza, José Carlos Lacerda De
Athiê, Emilio
Marigo, Carlos
Rahal, Fares
Fagundes, Djalma José [UNIFESP]
author_role author
author2 Athiê, Emilio
Marigo, Carlos
Rahal, Fares
Fagundes, Djalma José [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Santa Casa de São Paulo Faculdade de Medicina
Universidade Estadual de Londrina
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Souza, José Carlos Lacerda De
Athiê, Emilio
Marigo, Carlos
Rahal, Fares
Fagundes, Djalma José [UNIFESP]
dc.subject.por.fl_str_mv Spleen
Transplant
Splenosis
Wistar rats
Baço
Transplante esplênico
Esplenose
topic Spleen
Transplant
Splenosis
Wistar rats
Baço
Transplante esplênico
Esplenose
description PURPOSE: verify the cytological and histological aspects of the regeneration of fragments of autologous and heterotopic spleen implanted in peritoneal cavity and subcutaneous screen in Wistar albino rats. METHODS: forty four Wistar rats were assigned to one of two groups A(n=22) and B(n=22), which received their implants in subcutaneous screen and peritoneal cavity, respectively, under anesthesia. Those groups were redistributed in two other subgroups A1 (n=11) and B1(n=11), which received one and four splenic fragments in the areas of study. The remaining spleen was guided for cytological and histological processing, and was used as a control group. After five weeks, they were submitted to euthanasia, and peritoneal cavity opening took place as well as subcutaneous screen was taken off in order to search for regenerated splenic tissue. Cytological and histological assessment in the regenerated splenic tissue was performed, and the results were compared to the control group. Statistical analysis no parametric tests (p=>0,05) were used for. RESULTS: there were no statistically significant differences in relation to regenerated splenic tissue in subcutaneous screen (n=22) and the peritoneal cavity (n=22). In relation between presence and absence of regeneration, they're no statistically significant differences (p=0,182). Cytological and histological assessment did not show any statistically significant difference in relation to the control group. CONCLUSION: regeneration of autologous and heterotopic splenic tissue in mice is viable, and occurs frequently. Cytological and histological aspects are similar to normal spleen.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
2015-06-14T13:31:39Z
2015-06-14T13:31:39Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502005000300011
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005.
10.1590/S0102-86502005000300011
S0102-86502005000300011.pdf
0102-8650
S0102-86502005000300011
http://repositorio.unifesp.br/handle/11600/2583
url http://dx.doi.org/10.1590/S0102-86502005000300011
http://repositorio.unifesp.br/handle/11600/2583
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 20, n. 3, p. 253-257, 2005.
10.1590/S0102-86502005000300011
S0102-86502005000300011.pdf
0102-8650
S0102-86502005000300011
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 253-257
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268315036549120