Impact of doxorubicin treatment on the physiological functions of white adipose tissue
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57771 https://dx.doi.org/10.1371/journal.pone.0151548 |
Resumo: | White adipose tissue (WAT) plays a fundamental role in maintaining energy balance and important endocrine functions. The loss of WAT modifies adipokine secretion and disrupts homeostasis, potentially leading to severe metabolic effects and a reduced quality of life. Doxorubicin is a chemotherapeutic agent used clinically because of its good effectiveness against various types of cancer. However, doxorubicin has deleterious effects in many healthy tissues, including WAT, liver, and skeletal and cardiac muscles. Our objective was to investigate the effects of doxorubicin on white adipocytes through in vivo and in vitro experiments. Doxorubicin reduced the uptake of glucose by retroperitoneal adipocytes and 3T3-L1 cells via the inhibition of AMP-activated protein kinase Thr172 phosphorylation and glucose transporter 4 content. Doxorubicin also reduced the serum level of adiponectin and, to a greater extent, the expression of genes encoding lipogenic (Fas and Acc) and adipogenic factors (Pparg, C/ebpa, and Srebp1c) in retroperitoneal adipose tissue. In addition, doxorubicin inhibited both lipogenesis and lipolysis and reduced the hormone-sensitive lipase and adipose tissue triacylglycerol lipase protein levels. Therefore, our results demonstrate the impact of doxorubicin on WAT. These results are important to understand some side effects observed in patients receiving chemotherapy and should encourage new adjuvant treatments that aim to inhibit these side effects. |
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Biondo, Luana AmorimLima Junior, Edson AlvesSouza, Camila OliveiraCruz, Maysa Mariana [UNIFESP]Cunha, Roberta Dourado Cavalcante da [UNIFESP]Alonso-Vale, Maria Isabel Cardoso [UNIFESP]Oyama, Lila Missae [UNIFESP]Nascimento, Claudia Maria da Penha Oller do [UNIFESP]Pimentel, Gustavo DuarteSantos, Ronaldo Vagner Thomatieli dos [UNIFESP]Lira, Fabio Santos deRosa Neto, José Cesar2020-08-21T16:59:48Z2020-08-21T16:59:48Z2016Plos One. San Francisco, v. 11, n. 3, p. -, 2016.1932-6203https://repositorio.unifesp.br/handle/11600/57771https://dx.doi.org/10.1371/journal.pone.0151548WOS000372708900026.pdf10.1371/journal.pone.0151548WOS:000372708900026White adipose tissue (WAT) plays a fundamental role in maintaining energy balance and important endocrine functions. The loss of WAT modifies adipokine secretion and disrupts homeostasis, potentially leading to severe metabolic effects and a reduced quality of life. Doxorubicin is a chemotherapeutic agent used clinically because of its good effectiveness against various types of cancer. However, doxorubicin has deleterious effects in many healthy tissues, including WAT, liver, and skeletal and cardiac muscles. Our objective was to investigate the effects of doxorubicin on white adipocytes through in vivo and in vitro experiments. Doxorubicin reduced the uptake of glucose by retroperitoneal adipocytes and 3T3-L1 cells via the inhibition of AMP-activated protein kinase Thr172 phosphorylation and glucose transporter 4 content. Doxorubicin also reduced the serum level of adiponectin and, to a greater extent, the expression of genes encoding lipogenic (Fas and Acc) and adipogenic factors (Pparg, C/ebpa, and Srebp1c) in retroperitoneal adipose tissue. In addition, doxorubicin inhibited both lipogenesis and lipolysis and reduced the hormone-sensitive lipase and adipose tissue triacylglycerol lipase protein levels. Therefore, our results demonstrate the impact of doxorubicin on WAT. These results are important to understand some side effects observed in patients receiving chemotherapy and should encourage new adjuvant treatments that aim to inhibit these side effects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Inst Biomed Sci, Dept Cellular Biol & Dev, Immunometab Res Grp, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Physiol, Physiol Nutr Discipline, Sao Paulo, SP, BrazilUniv Fed Goias, Fac Nursing & Nutr, Goiania, Go, BrazilUniv Fed Sao Paulo UNIFESP, Dept Psychobiol, Sao Paulo, SP, BrazilState Univ Sao Paulo Julio de Mesquita Filho UNES, Dept Phys Educ, Presidente Prudente, SP, BrazilFed Univ Sao Paulo UNIFESP, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Physiol, Physiol Nutr Discipline, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Psychobiol, Sao Paulo, SP, BrazilFAPESP: 2013/09367-4Web of ScienceengPublic Library SciencePlos OneImpact of doxorubicin treatment on the physiological functions of white adipose tissueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSan Francisco113info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000372708900026.pdfapplication/pdf1782237${dspace.ui.url}/bitstream/11600/57771/1/WOS000372708900026.pdf6dd19d323d9d9ededcd9d6ec27f0a9d3MD51open accessTEXTWOS000372708900026.pdf.txtWOS000372708900026.pdf.txtExtracted texttext/plain50348${dspace.ui.url}/bitstream/11600/57771/8/WOS000372708900026.pdf.txtcbc2bf1cd99da3aef937d3905b0695cdMD58open accessTHUMBNAILWOS000372708900026.pdf.jpgWOS000372708900026.pdf.jpgIM Thumbnailimage/jpeg7519${dspace.ui.url}/bitstream/11600/57771/10/WOS000372708900026.pdf.jpgbf512b7f3d0a5d09c3dfb659b11191feMD510open access11600/577712023-06-05 19:34:44.295open accessoai:repositorio.unifesp.br:11600/57771Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:34:44Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
title |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
spellingShingle |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue Biondo, Luana Amorim |
title_short |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
title_full |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
title_fullStr |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
title_full_unstemmed |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
title_sort |
Impact of doxorubicin treatment on the physiological functions of white adipose tissue |
author |
Biondo, Luana Amorim |
author_facet |
Biondo, Luana Amorim Lima Junior, Edson Alves Souza, Camila Oliveira Cruz, Maysa Mariana [UNIFESP] Cunha, Roberta Dourado Cavalcante da [UNIFESP] Alonso-Vale, Maria Isabel Cardoso [UNIFESP] Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pimentel, Gustavo Duarte Santos, Ronaldo Vagner Thomatieli dos [UNIFESP] Lira, Fabio Santos de Rosa Neto, José Cesar |
author_role |
author |
author2 |
Lima Junior, Edson Alves Souza, Camila Oliveira Cruz, Maysa Mariana [UNIFESP] Cunha, Roberta Dourado Cavalcante da [UNIFESP] Alonso-Vale, Maria Isabel Cardoso [UNIFESP] Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pimentel, Gustavo Duarte Santos, Ronaldo Vagner Thomatieli dos [UNIFESP] Lira, Fabio Santos de Rosa Neto, José Cesar |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Biondo, Luana Amorim Lima Junior, Edson Alves Souza, Camila Oliveira Cruz, Maysa Mariana [UNIFESP] Cunha, Roberta Dourado Cavalcante da [UNIFESP] Alonso-Vale, Maria Isabel Cardoso [UNIFESP] Oyama, Lila Missae [UNIFESP] Nascimento, Claudia Maria da Penha Oller do [UNIFESP] Pimentel, Gustavo Duarte Santos, Ronaldo Vagner Thomatieli dos [UNIFESP] Lira, Fabio Santos de Rosa Neto, José Cesar |
description |
White adipose tissue (WAT) plays a fundamental role in maintaining energy balance and important endocrine functions. The loss of WAT modifies adipokine secretion and disrupts homeostasis, potentially leading to severe metabolic effects and a reduced quality of life. Doxorubicin is a chemotherapeutic agent used clinically because of its good effectiveness against various types of cancer. However, doxorubicin has deleterious effects in many healthy tissues, including WAT, liver, and skeletal and cardiac muscles. Our objective was to investigate the effects of doxorubicin on white adipocytes through in vivo and in vitro experiments. Doxorubicin reduced the uptake of glucose by retroperitoneal adipocytes and 3T3-L1 cells via the inhibition of AMP-activated protein kinase Thr172 phosphorylation and glucose transporter 4 content. Doxorubicin also reduced the serum level of adiponectin and, to a greater extent, the expression of genes encoding lipogenic (Fas and Acc) and adipogenic factors (Pparg, C/ebpa, and Srebp1c) in retroperitoneal adipose tissue. In addition, doxorubicin inhibited both lipogenesis and lipolysis and reduced the hormone-sensitive lipase and adipose tissue triacylglycerol lipase protein levels. Therefore, our results demonstrate the impact of doxorubicin on WAT. These results are important to understand some side effects observed in patients receiving chemotherapy and should encourage new adjuvant treatments that aim to inhibit these side effects. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-21T16:59:48Z |
dc.date.available.fl_str_mv |
2020-08-21T16:59:48Z |
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dc.identifier.citation.fl_str_mv |
Plos One. San Francisco, v. 11, n. 3, p. -, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57771 https://dx.doi.org/10.1371/journal.pone.0151548 |
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1932-6203 |
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WOS000372708900026.pdf |
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10.1371/journal.pone.0151548 |
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WOS:000372708900026 |
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Plos One. San Francisco, v. 11, n. 3, p. -, 2016. 1932-6203 WOS000372708900026.pdf 10.1371/journal.pone.0151548 WOS:000372708900026 |
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https://repositorio.unifesp.br/handle/11600/57771 https://dx.doi.org/10.1371/journal.pone.0151548 |
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