Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27486 http://dx.doi.org/10.1086/379666 |
Resumo: | The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total of 118 typical and atypical strains of EPEC serotypes and non-EPEC serogroups isolated from children with or without acute diarrhea who were from different cities in Brazil were examined for virulence-associated markers and adherence to HEp-2 cells, and also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical strains were identical to typical strains with regard to the virulence factors encoded on the locus of enterocyte effacement (LEE). in contrast with typical EPEC strains, none of the atypical strains reacted with the bfpA probe, and half of the strains hybridized with the perA probe. Most atypical strains presented Tir sequences that correlated with enteropathogenic or enterohemorrhagic E. coli (98%), had LEE inserted in either selC or pheU (88%), and presented a typeable intimin (52%). Eighteen new serotypes were found in the EPEC strains. Atypical and typical EPEC strains belonged to different RAPD clusters. Most atypical strains showed a localized-like adherence pattern (61.5%). of the non-LEE-encoded virulence factors, enteroaggregative E. coli heat-stable enterotoxin was noted most frequently (45%) and was significantly associated with diarrhea (P=.01). Thus, this virulence marker may be used as an additional tool for the diagnosis of truly atypical pathogenic strains. |
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Dulguer, Michelle Vanzella [UNIFESP]Fabbricotti, Sandra Hilde [UNIFESP]Bando, S. Y.Moreira, C. A.Fagundes Neto, Ulysses [UNIFESP]Scaletsky, Isabel Cristina Affonso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T12:34:08Z2016-01-24T12:34:08Z2003-12-01Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 188, n. 11, p. 1685-1694, 2003.0022-1899http://repositorio.unifesp.br/handle/11600/27486http://dx.doi.org/10.1086/379666WOS000187493200011.pdf10.1086/379666WOS:000187493200011The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total of 118 typical and atypical strains of EPEC serotypes and non-EPEC serogroups isolated from children with or without acute diarrhea who were from different cities in Brazil were examined for virulence-associated markers and adherence to HEp-2 cells, and also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical strains were identical to typical strains with regard to the virulence factors encoded on the locus of enterocyte effacement (LEE). in contrast with typical EPEC strains, none of the atypical strains reacted with the bfpA probe, and half of the strains hybridized with the perA probe. Most atypical strains presented Tir sequences that correlated with enteropathogenic or enterohemorrhagic E. coli (98%), had LEE inserted in either selC or pheU (88%), and presented a typeable intimin (52%). Eighteen new serotypes were found in the EPEC strains. Atypical and typical EPEC strains belonged to different RAPD clusters. Most atypical strains showed a localized-like adherence pattern (61.5%). of the non-LEE-encoded virulence factors, enteroaggregative E. coli heat-stable enterotoxin was noted most frequently (45%) and was significantly associated with diarrhea (P=.01). Thus, this virulence marker may be used as an additional tool for the diagnosis of truly atypical pathogenic strains.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pediat, BR-04023062 São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pediat, BR-04023062 São Paulo, BrazilWeb of Science1685-1694engUniv Chicago PressJournal of Infectious DiseasesAtypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrheainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000187493200011.pdfapplication/pdf189568${dspace.ui.url}/bitstream/11600/27486/1/WOS000187493200011.pdf50deac52e9e67e43dd4e347677ff5a2aMD51open accessTEXTWOS000187493200011.pdf.txtWOS000187493200011.pdf.txtExtracted texttext/plain47412${dspace.ui.url}/bitstream/11600/27486/2/WOS000187493200011.pdf.txt959652c104a754576f6e981bf327e734MD52open access11600/274862022-09-27 11:31:15.821open accessoai:repositorio.unifesp.br:11600/27486Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T14:31:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
title |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
spellingShingle |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea Dulguer, Michelle Vanzella [UNIFESP] |
title_short |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
title_full |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
title_fullStr |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
title_full_unstemmed |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
title_sort |
Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea |
author |
Dulguer, Michelle Vanzella [UNIFESP] |
author_facet |
Dulguer, Michelle Vanzella [UNIFESP] Fabbricotti, Sandra Hilde [UNIFESP] Bando, S. Y. Moreira, C. A. Fagundes Neto, Ulysses [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author_role |
author |
author2 |
Fabbricotti, Sandra Hilde [UNIFESP] Bando, S. Y. Moreira, C. A. Fagundes Neto, Ulysses [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dulguer, Michelle Vanzella [UNIFESP] Fabbricotti, Sandra Hilde [UNIFESP] Bando, S. Y. Moreira, C. A. Fagundes Neto, Ulysses [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
description |
The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total of 118 typical and atypical strains of EPEC serotypes and non-EPEC serogroups isolated from children with or without acute diarrhea who were from different cities in Brazil were examined for virulence-associated markers and adherence to HEp-2 cells, and also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical strains were identical to typical strains with regard to the virulence factors encoded on the locus of enterocyte effacement (LEE). in contrast with typical EPEC strains, none of the atypical strains reacted with the bfpA probe, and half of the strains hybridized with the perA probe. Most atypical strains presented Tir sequences that correlated with enteropathogenic or enterohemorrhagic E. coli (98%), had LEE inserted in either selC or pheU (88%), and presented a typeable intimin (52%). Eighteen new serotypes were found in the EPEC strains. Atypical and typical EPEC strains belonged to different RAPD clusters. Most atypical strains showed a localized-like adherence pattern (61.5%). of the non-LEE-encoded virulence factors, enteroaggregative E. coli heat-stable enterotoxin was noted most frequently (45%) and was significantly associated with diarrhea (P=.01). Thus, this virulence marker may be used as an additional tool for the diagnosis of truly atypical pathogenic strains. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:34:08Z |
dc.date.available.fl_str_mv |
2016-01-24T12:34:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 188, n. 11, p. 1685-1694, 2003. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27486 http://dx.doi.org/10.1086/379666 |
dc.identifier.issn.none.fl_str_mv |
0022-1899 |
dc.identifier.file.none.fl_str_mv |
WOS000187493200011.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1086/379666 |
dc.identifier.wos.none.fl_str_mv |
WOS:000187493200011 |
identifier_str_mv |
Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 188, n. 11, p. 1685-1694, 2003. 0022-1899 WOS000187493200011.pdf 10.1086/379666 WOS:000187493200011 |
url |
http://repositorio.unifesp.br/handle/11600/27486 http://dx.doi.org/10.1086/379666 |
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eng |
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Univ Chicago Press |
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Univ Chicago Press |
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