Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/31036 http://dx.doi.org/10.1074/jbc.M801990200 |
Resumo: | The role of intracellular Ca2+ (Ca-i(2+)) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca-i(2+) concentration ([Ca2+](i)) with activation of phospholipase C gamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. in addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1(+) population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+](i), moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit(+) population, and differentiation into macrophages without participation of GJ. It is likely that Ca-i(2+) participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+](i) would be related to cytokine-dependent proliferation with participation of GJ, whereas high increases in [Ca2+](i) would be related to macrophage differentiation without maintenance of the primitive population. |
id |
UFSP_9b3be4f613363f5ea8dc53b28605a4ef |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/31036 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Paredes-Gamero, Edgar Julian [UNIFESP]Leon, Carlos M. M. P. [UNIFESP]Borojevic, RadovanOshiro, Maria Etsuko Miyamoto [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)2016-01-24T13:51:53Z2016-01-24T13:51:53Z2008-11-14Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 283, n. 46, p. 31909-31919, 2008.0021-9258http://repositorio.unifesp.br/handle/11600/31036http://dx.doi.org/10.1074/jbc.M80199020010.1074/jbc.M801990200WOS:000260760800073The role of intracellular Ca2+ (Ca-i(2+)) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca-i(2+) concentration ([Ca2+](i)) with activation of phospholipase C gamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. in addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1(+) population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+](i), moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit(+) population, and differentiation into macrophages without participation of GJ. It is likely that Ca-i(2+) participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+](i) would be related to cytokine-dependent proliferation with participation of GJ, whereas high increases in [Ca2+](i) would be related to macrophage differentiation without maintenance of the primitive population.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Biophys, BR-04023062 São Paulo, BrazilUniv Fed Rio de Janeiro, Dept Histol & Embryol, BR-21941970 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04023062 São Paulo, BrazilWeb of Science31909-31919engAmer Soc Biochemistry Molecular Biology IncJournal of Biological ChemistryChanges in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/310362022-11-04 15:31:39.65metadata only accessoai:repositorio.unifesp.br:11600/31036Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T18:31:39Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
title |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
spellingShingle |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells Paredes-Gamero, Edgar Julian [UNIFESP] |
title_short |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
title_full |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
title_fullStr |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
title_full_unstemmed |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
title_sort |
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells |
author |
Paredes-Gamero, Edgar Julian [UNIFESP] |
author_facet |
Paredes-Gamero, Edgar Julian [UNIFESP] Leon, Carlos M. M. P. [UNIFESP] Borojevic, Radovan Oshiro, Maria Etsuko Miyamoto [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
author_role |
author |
author2 |
Leon, Carlos M. M. P. [UNIFESP] Borojevic, Radovan Oshiro, Maria Etsuko Miyamoto [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal do Rio de Janeiro (UFRJ) |
dc.contributor.author.fl_str_mv |
Paredes-Gamero, Edgar Julian [UNIFESP] Leon, Carlos M. M. P. [UNIFESP] Borojevic, Radovan Oshiro, Maria Etsuko Miyamoto [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
description |
The role of intracellular Ca2+ (Ca-i(2+)) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca-i(2+) concentration ([Ca2+](i)) with activation of phospholipase C gamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. in addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1(+) population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+](i), moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit(+) population, and differentiation into macrophages without participation of GJ. It is likely that Ca-i(2+) participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+](i) would be related to cytokine-dependent proliferation with participation of GJ, whereas high increases in [Ca2+](i) would be related to macrophage differentiation without maintenance of the primitive population. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-11-14 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:51:53Z |
dc.date.available.fl_str_mv |
2016-01-24T13:51:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 283, n. 46, p. 31909-31919, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/31036 http://dx.doi.org/10.1074/jbc.M801990200 |
dc.identifier.issn.none.fl_str_mv |
0021-9258 |
dc.identifier.doi.none.fl_str_mv |
10.1074/jbc.M801990200 |
dc.identifier.wos.none.fl_str_mv |
WOS:000260760800073 |
identifier_str_mv |
Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 283, n. 46, p. 31909-31919, 2008. 0021-9258 10.1074/jbc.M801990200 WOS:000260760800073 |
url |
http://repositorio.unifesp.br/handle/11600/31036 http://dx.doi.org/10.1074/jbc.M801990200 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Biological Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
31909-31919 |
dc.publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764185666322432 |