Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/37089 https://dx.doi.org/10.1186/1475-2867-13-123 |
Resumo: | Over the last few years, studies have suggested that oxidative stress plays a role in the regulation of hematopoietic cell homeostasis. in particular, the effects of hydrogen peroxide (H2O2) range from hematopoietic cell proliferation to cell death, depending on its concentration in the intracellular milieu. in this work, we evaluated the effects of an oxidative environment on normal and leukemic hematopoietic cells by stimulating normal human (umbilical cord blood) and murine (bone marrow) hematopoietic cells, as well as human myeloid leukemic cells (HL-60 lineage), upon H2O2 stimulus. Total cell populations and primitive subsets were evaluated for each cell type. H2O2 stimulus induces HL-60 cell death, whereas the viability of human and murine normal cells was not affected. the effects of H2O2 stimulus on hematopoietic stem/progenitor cell subsets were examined and the normal primitive cells were found to be unaffected; however, the percentage of leukemic stem cells (LSC) increased in response to H2O2, while clonogenic ability of these cells to generate myeloid clones was inhibited. in addition, H2O2 stimulus caused a decrease in the levels of p-AKT in HL-60 cells, which most likely mediates the observed decrease of viability. in summary, we found that at low concentrations, H2O2 preferentially affects both the LSC subset and total HL-60 cells without damage normal cells. |
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Nogueira-Pedro, Amanda [UNIFESP]Cesario, Thalyta Aparecida Munhoz [UNIFESP]Dias, Carolina Carvalho [UNIFESP]Origassa, Clarice Silvia Taemi [UNIFESP]Eça, Lilian Piñero MarcolinParedes-Gamero, Edgar Julian [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Universidade Federal de São Paulo (UNIFESP)IPCTRON Stem Cell Res Inst2016-01-24T14:34:52Z2016-01-24T14:34:52Z2013-12-23Cancer Cell International. London: Biomed Central Ltd, v. 13, 9 p., 2013.1475-2867https://repositorio.unifesp.br/handle/11600/37089https://dx.doi.org/10.1186/1475-2867-13-123WOS000329784700001.pdf10.1186/1475-2867-13-123WOS:000329784700001Over the last few years, studies have suggested that oxidative stress plays a role in the regulation of hematopoietic cell homeostasis. in particular, the effects of hydrogen peroxide (H2O2) range from hematopoietic cell proliferation to cell death, depending on its concentration in the intracellular milieu. in this work, we evaluated the effects of an oxidative environment on normal and leukemic hematopoietic cells by stimulating normal human (umbilical cord blood) and murine (bone marrow) hematopoietic cells, as well as human myeloid leukemic cells (HL-60 lineage), upon H2O2 stimulus. Total cell populations and primitive subsets were evaluated for each cell type. H2O2 stimulus induces HL-60 cell death, whereas the viability of human and murine normal cells was not affected. the effects of H2O2 stimulus on hematopoietic stem/progenitor cell subsets were examined and the normal primitive cells were found to be unaffected; however, the percentage of leukemic stem cells (LSC) increased in response to H2O2, while clonogenic ability of these cells to generate myeloid clones was inhibited. in addition, H2O2 stimulus caused a decrease in the levels of p-AKT in HL-60 cells, which most likely mediates the observed decrease of viability. in summary, we found that at low concentrations, H2O2 preferentially affects both the LSC subset and total HL-60 cells without damage normal cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Dept Biophys, BR-04062023 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04062023 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, BR-04062023 São Paulo, BrazilIPCTRON Stem Cell Res Inst, BR-04037000 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04062023 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biochem, BR-04062023 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Med, BR-04062023 São Paulo, BrazilUniversidade Federal de São Paulo, BR-04039032 São Paulo, BrazilFAPESP: 2009/52852-5Web of Science9engBiomed Central LtdCancer Cell InternationalHematopoietic stem cellHL-60Leukemic stem cellHydrogen peroxideHydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent mannerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000329784700001.pdfapplication/pdf1271220${dspace.ui.url}/bitstream/11600/37089/1/WOS000329784700001.pdf488cea4b6191150c45e692f4f7c0e332MD51open accessTEXTWOS000329784700001.pdf.txtWOS000329784700001.pdf.txtExtracted texttext/plain39703${dspace.ui.url}/bitstream/11600/37089/2/WOS000329784700001.pdf.txt3b88df218aa13b4da803920d20de5e7cMD52open access11600/370892023-10-16 19:34:15.538open accessoai:repositorio.unifesp.br:11600/37089Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-10-16T22:34:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
title |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
spellingShingle |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner Nogueira-Pedro, Amanda [UNIFESP] Hematopoietic stem cell HL-60 Leukemic stem cell Hydrogen peroxide |
title_short |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
title_full |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
title_fullStr |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
title_full_unstemmed |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
title_sort |
Hydrogen peroxide (H2O2) induces leukemic but not normal hematopoietic cell death in a dose-dependent manner |
author |
Nogueira-Pedro, Amanda [UNIFESP] |
author_facet |
Nogueira-Pedro, Amanda [UNIFESP] Cesario, Thalyta Aparecida Munhoz [UNIFESP] Dias, Carolina Carvalho [UNIFESP] Origassa, Clarice Silvia Taemi [UNIFESP] Eça, Lilian Piñero Marcolin Paredes-Gamero, Edgar Julian [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
author_role |
author |
author2 |
Cesario, Thalyta Aparecida Munhoz [UNIFESP] Dias, Carolina Carvalho [UNIFESP] Origassa, Clarice Silvia Taemi [UNIFESP] Eça, Lilian Piñero Marcolin Paredes-Gamero, Edgar Julian [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) IPCTRON Stem Cell Res Inst |
dc.contributor.author.fl_str_mv |
Nogueira-Pedro, Amanda [UNIFESP] Cesario, Thalyta Aparecida Munhoz [UNIFESP] Dias, Carolina Carvalho [UNIFESP] Origassa, Clarice Silvia Taemi [UNIFESP] Eça, Lilian Piñero Marcolin Paredes-Gamero, Edgar Julian [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] |
dc.subject.eng.fl_str_mv |
Hematopoietic stem cell HL-60 Leukemic stem cell Hydrogen peroxide |
topic |
Hematopoietic stem cell HL-60 Leukemic stem cell Hydrogen peroxide |
description |
Over the last few years, studies have suggested that oxidative stress plays a role in the regulation of hematopoietic cell homeostasis. in particular, the effects of hydrogen peroxide (H2O2) range from hematopoietic cell proliferation to cell death, depending on its concentration in the intracellular milieu. in this work, we evaluated the effects of an oxidative environment on normal and leukemic hematopoietic cells by stimulating normal human (umbilical cord blood) and murine (bone marrow) hematopoietic cells, as well as human myeloid leukemic cells (HL-60 lineage), upon H2O2 stimulus. Total cell populations and primitive subsets were evaluated for each cell type. H2O2 stimulus induces HL-60 cell death, whereas the viability of human and murine normal cells was not affected. the effects of H2O2 stimulus on hematopoietic stem/progenitor cell subsets were examined and the normal primitive cells were found to be unaffected; however, the percentage of leukemic stem cells (LSC) increased in response to H2O2, while clonogenic ability of these cells to generate myeloid clones was inhibited. in addition, H2O2 stimulus caused a decrease in the levels of p-AKT in HL-60 cells, which most likely mediates the observed decrease of viability. in summary, we found that at low concentrations, H2O2 preferentially affects both the LSC subset and total HL-60 cells without damage normal cells. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-23 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:52Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Cancer Cell International. London: Biomed Central Ltd, v. 13, 9 p., 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/37089 https://dx.doi.org/10.1186/1475-2867-13-123 |
dc.identifier.issn.none.fl_str_mv |
1475-2867 |
dc.identifier.file.none.fl_str_mv |
WOS000329784700001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1475-2867-13-123 |
dc.identifier.wos.none.fl_str_mv |
WOS:000329784700001 |
identifier_str_mv |
Cancer Cell International. London: Biomed Central Ltd, v. 13, 9 p., 2013. 1475-2867 WOS000329784700001.pdf 10.1186/1475-2867-13-123 WOS:000329784700001 |
url |
https://repositorio.unifesp.br/handle/11600/37089 https://dx.doi.org/10.1186/1475-2867-13-123 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Cancer Cell International |
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openAccess |
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9 |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
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