Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.brainres.2015.12.055 https://repositorio.unifesp.br/handle/11600/57873 |
Resumo: | The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved. |
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Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsyEpilepsySeizure frequencyHippocampusNeuronal cellNon-neuronal cellThe relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.Univ Fed Sao Paulo UNIFESP, Dept Fisiol, Ed Ciencias Biomed, Rua Botucatu 862,5 Andar Vila Clementino, BR-04023900 Sao Paulo, SP, BrazilHosp Israelita Albert Einstein, Inst Cerebro INCE, Sao Paulo, SP, BrazilUniv Fed Rio de Janeiro, Inst Ciencias Biomed, Rio De Janeiro, RJ, BrazilUniv Fed Sao Paulo UNIFESP, Dept Neurol & Neurocirurgia, Sao Paulo, SP, BrazilUniv Mogi das Cruzes, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Fisiol, Ed Ciencias Biomed, Rua Botucatu 862,5 Andar Vila Clementino, BR-04023900 Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Neurol & Neurocirurgia, Sao Paulo, SP, BrazilWeb of ScienceCAPESFAPESPCNPqInstituto Nacional de Neurociencia Translacional (INNT)Elsevier Science Bv2020-08-21T17:00:07Z2020-08-21T17:00:07Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion179-186application/pdfhttp://dx.doi.org/10.1016/j.brainres.2015.12.055Brain Research. Amsterdam, v. 1634, p. 179-186, 2016.10.1016/j.brainres.2015.12.055WOS000372688300018.pdf0006-8993https://repositorio.unifesp.br/handle/11600/57873WOS:000372688300018engBrain ResearchAmsterdaminfo:eu-repo/semantics/openAccessLopim, Glauber Menezes [UNIFESP]Campos, Diego Vannucci [UNIFESP]da Silva, Sergio Gomesde Almeida, Alexandre Aparecido [UNIFESP]Lent, RobertoCavalheiro, Esper Abrao [UNIFESP]Arida, Ricardo Mario [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-09T05:09:03Zoai:repositorio.unifesp.br/:11600/57873Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-09T05:09:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
title |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
spellingShingle |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy Lopim, Glauber Menezes [UNIFESP] Epilepsy Seizure frequency Hippocampus Neuronal cell Non-neuronal cell |
title_short |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
title_full |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
title_fullStr |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
title_full_unstemmed |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
title_sort |
Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy |
author |
Lopim, Glauber Menezes [UNIFESP] |
author_facet |
Lopim, Glauber Menezes [UNIFESP] Campos, Diego Vannucci [UNIFESP] da Silva, Sergio Gomes de Almeida, Alexandre Aparecido [UNIFESP] Lent, Roberto Cavalheiro, Esper Abrao [UNIFESP] Arida, Ricardo Mario [UNIFESP] |
author_role |
author |
author2 |
Campos, Diego Vannucci [UNIFESP] da Silva, Sergio Gomes de Almeida, Alexandre Aparecido [UNIFESP] Lent, Roberto Cavalheiro, Esper Abrao [UNIFESP] Arida, Ricardo Mario [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Lopim, Glauber Menezes [UNIFESP] Campos, Diego Vannucci [UNIFESP] da Silva, Sergio Gomes de Almeida, Alexandre Aparecido [UNIFESP] Lent, Roberto Cavalheiro, Esper Abrao [UNIFESP] Arida, Ricardo Mario [UNIFESP] |
dc.subject.por.fl_str_mv |
Epilepsy Seizure frequency Hippocampus Neuronal cell Non-neuronal cell |
topic |
Epilepsy Seizure frequency Hippocampus Neuronal cell Non-neuronal cell |
description |
The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2020-08-21T17:00:07Z 2020-08-21T17:00:07Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.brainres.2015.12.055 Brain Research. Amsterdam, v. 1634, p. 179-186, 2016. 10.1016/j.brainres.2015.12.055 WOS000372688300018.pdf 0006-8993 https://repositorio.unifesp.br/handle/11600/57873 WOS:000372688300018 |
url |
http://dx.doi.org/10.1016/j.brainres.2015.12.055 https://repositorio.unifesp.br/handle/11600/57873 |
identifier_str_mv |
Brain Research. Amsterdam, v. 1634, p. 179-186, 2016. 10.1016/j.brainres.2015.12.055 WOS000372688300018.pdf 0006-8993 WOS:000372688300018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
179-186 application/pdf |
dc.coverage.none.fl_str_mv |
Amsterdam |
dc.publisher.none.fl_str_mv |
Elsevier Science Bv |
publisher.none.fl_str_mv |
Elsevier Science Bv |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268268952682496 |