Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy

Detalhes bibliográficos
Autor(a) principal: Lopim, Glauber Menezes [UNIFESP]
Data de Publicação: 2016
Outros Autores: Campos, Diego Vannucci [UNIFESP], da Silva, Sergio Gomes, de Almeida, Alexandre Aparecido [UNIFESP], Lent, Roberto, Cavalheiro, Esper Abrao [UNIFESP], Arida, Ricardo Mario [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.brainres.2015.12.055
https://repositorio.unifesp.br/handle/11600/57873
Resumo: The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.
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spelling Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsyEpilepsySeizure frequencyHippocampusNeuronal cellNon-neuronal cellThe relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.Univ Fed Sao Paulo UNIFESP, Dept Fisiol, Ed Ciencias Biomed, Rua Botucatu 862,5 Andar Vila Clementino, BR-04023900 Sao Paulo, SP, BrazilHosp Israelita Albert Einstein, Inst Cerebro INCE, Sao Paulo, SP, BrazilUniv Fed Rio de Janeiro, Inst Ciencias Biomed, Rio De Janeiro, RJ, BrazilUniv Fed Sao Paulo UNIFESP, Dept Neurol & Neurocirurgia, Sao Paulo, SP, BrazilUniv Mogi das Cruzes, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Fisiol, Ed Ciencias Biomed, Rua Botucatu 862,5 Andar Vila Clementino, BR-04023900 Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Dept Neurol & Neurocirurgia, Sao Paulo, SP, BrazilWeb of ScienceCAPESFAPESPCNPqInstituto Nacional de Neurociencia Translacional (INNT)Elsevier Science Bv2020-08-21T17:00:07Z2020-08-21T17:00:07Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion179-186application/pdfhttp://dx.doi.org/10.1016/j.brainres.2015.12.055Brain Research. Amsterdam, v. 1634, p. 179-186, 2016.10.1016/j.brainres.2015.12.055WOS000372688300018.pdf0006-8993https://repositorio.unifesp.br/handle/11600/57873WOS:000372688300018engBrain ResearchAmsterdaminfo:eu-repo/semantics/openAccessLopim, Glauber Menezes [UNIFESP]Campos, Diego Vannucci [UNIFESP]da Silva, Sergio Gomesde Almeida, Alexandre Aparecido [UNIFESP]Lent, RobertoCavalheiro, Esper Abrao [UNIFESP]Arida, Ricardo Mario [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-09T05:09:03Zoai:repositorio.unifesp.br/:11600/57873Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-09T05:09:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
title Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
spellingShingle Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
Lopim, Glauber Menezes [UNIFESP]
Epilepsy
Seizure frequency
Hippocampus
Neuronal cell
Non-neuronal cell
title_short Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
title_full Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
title_fullStr Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
title_full_unstemmed Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
title_sort Relationship between seizure frequency and number of neuronal and non-neuronal cells in the hippocampus throughout the life of rats with epilepsy
author Lopim, Glauber Menezes [UNIFESP]
author_facet Lopim, Glauber Menezes [UNIFESP]
Campos, Diego Vannucci [UNIFESP]
da Silva, Sergio Gomes
de Almeida, Alexandre Aparecido [UNIFESP]
Lent, Roberto
Cavalheiro, Esper Abrao [UNIFESP]
Arida, Ricardo Mario [UNIFESP]
author_role author
author2 Campos, Diego Vannucci [UNIFESP]
da Silva, Sergio Gomes
de Almeida, Alexandre Aparecido [UNIFESP]
Lent, Roberto
Cavalheiro, Esper Abrao [UNIFESP]
Arida, Ricardo Mario [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopim, Glauber Menezes [UNIFESP]
Campos, Diego Vannucci [UNIFESP]
da Silva, Sergio Gomes
de Almeida, Alexandre Aparecido [UNIFESP]
Lent, Roberto
Cavalheiro, Esper Abrao [UNIFESP]
Arida, Ricardo Mario [UNIFESP]
dc.subject.por.fl_str_mv Epilepsy
Seizure frequency
Hippocampus
Neuronal cell
Non-neuronal cell
topic Epilepsy
Seizure frequency
Hippocampus
Neuronal cell
Non-neuronal cell
description The relationship between seizure frequency and cell death has been a subject of controversy. To tackle this issue, we determined the frequency of seizures and the total number of hippocampal cells throughout the life of rats with epilepsy using the pilocarpine model. Seizure frequency varied in animals with epilepsy according to which period of life they were in, with a progressive increase in the number of seizures until 180 days (sixth months) of epileptic life followed by a decrease (330 days-eleventh month) and subsequently stabilization of seizures. Cell counts by means of isotropic fractionation showed a reduction in the number of hippocampal neuronal cells following 30, 90, 180 and 360 days of spontaneous recurrent seizures (SRS) in rats compared to their controls (about 25%-30% of neuronal cell reduction). In addition, animals with 360 days of SRS showed a reduction in the number of neuronal cells when compared with animals with 90 and 180 days of seizures. The total number of hippocampal non-neuronal cells was reduced in rats with epilepsy after 30 days of SRS, but no significant alteration was observed on the 90th, 180th and 360th days. The total number of neuronal cells was negatively correlated with seizure frequency, indicating an association between occurrence of epileptic seizures throughout life and neuronal loss. In sum, our results add novel data to the literature concerning the time-course of SRS and hippocampal cell number throughout epileptic life. (C) 2016 Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-08-21T17:00:07Z
2020-08-21T17:00:07Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.brainres.2015.12.055
Brain Research. Amsterdam, v. 1634, p. 179-186, 2016.
10.1016/j.brainres.2015.12.055
WOS000372688300018.pdf
0006-8993
https://repositorio.unifesp.br/handle/11600/57873
WOS:000372688300018
url http://dx.doi.org/10.1016/j.brainres.2015.12.055
https://repositorio.unifesp.br/handle/11600/57873
identifier_str_mv Brain Research. Amsterdam, v. 1634, p. 179-186, 2016.
10.1016/j.brainres.2015.12.055
WOS000372688300018.pdf
0006-8993
WOS:000372688300018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brain Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 179-186
application/pdf
dc.coverage.none.fl_str_mv Amsterdam
dc.publisher.none.fl_str_mv Elsevier Science Bv
publisher.none.fl_str_mv Elsevier Science Bv
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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