Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37987 http://dx.doi.org/10.1186/1471-2180-14-184 |
Resumo: | Background: Biofilm formation by enteropathogenic Escherichia coli (EPEC) have been recently described in the prototype typical EPEC E2348/69 strain and in an atypical EPEC O55:H7 strain. in this study, we sought to evaluate biofilm formation in a collection of 126 atypical EPEC strains isolated from 92 diarrheic and 34 nondiarrheic children, belonging to different serotypes. the association of biofilm formation and adhesin-related genes were also investigated.Results: Biofilm formation occurred in 37 (29%)strains of different serotypes, when the assays were performed at 26 degrees C and 37 degrees C for 24 h. Among these, four strains (A79, A87, A88, and A111) formed a stronger biofilm than did the others. the frequency of biofilm producers was higher among isolates from patients compared with isolates from controls (34.8% vs 14.7%; P = 0.029). An association was found between biofilm formation and expression of type 1 fimbriae and curli (P < 0.05). Unlike the previously described aEPEC O55:H7, one aEPEC O119:HND strain (A111) formed a strong biofilm and pellicle at the air-liquid interface, but did not express curli. Transposon mutagenesis was used to identify biofilm-deficient mutants. Transposon insertion sequences of six mutants revealed similarity with type 1 fimbriae (fimC, fimD, and fimH), diguanylate cyclase, ATP synthase F1, beta subunit (atpD), and the uncharacterized YjiC protein. All these mutants were deficient in biofilm formation ability.Conclusion: This study showed that the ability to adhere to abiotic surfaces and form biofilm is present in an array of aEPEC strains. Moreover, it seems that the ability to form biofilms is associated with the presence of type 1 fimbriae and diguanylate cyclase. Characterization of additional biofilm formation mutants may reveal other mechanisms involved in biofilm formation and bring new insights into aEPEC adhesion and pathogenesis. |
id |
UFSP_9f1535dd4506e04c5344f79fed7e95b8 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/37987 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Nascimento, Heloisa H. [UNIFESP]Silva, Lucas E. P. [UNIFESP]Souza, Renata T. [UNIFESP]Silva, Neusa Pereira da [UNIFESP]Scaletsky, Isabel Cristina Affonso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:37:35Z2016-01-24T14:37:35Z2014-07-10Bmc Microbiology. London: Biomed Central Ltd, v. 14, 7 p., 2014.1471-2180http://repositorio.unifesp.br/handle/11600/37987http://dx.doi.org/10.1186/1471-2180-14-184WOS000339080400001.pdf10.1186/1471-2180-14-184WOS:000339080400001Background: Biofilm formation by enteropathogenic Escherichia coli (EPEC) have been recently described in the prototype typical EPEC E2348/69 strain and in an atypical EPEC O55:H7 strain. in this study, we sought to evaluate biofilm formation in a collection of 126 atypical EPEC strains isolated from 92 diarrheic and 34 nondiarrheic children, belonging to different serotypes. the association of biofilm formation and adhesin-related genes were also investigated.Results: Biofilm formation occurred in 37 (29%)strains of different serotypes, when the assays were performed at 26 degrees C and 37 degrees C for 24 h. Among these, four strains (A79, A87, A88, and A111) formed a stronger biofilm than did the others. the frequency of biofilm producers was higher among isolates from patients compared with isolates from controls (34.8% vs 14.7%; P = 0.029). An association was found between biofilm formation and expression of type 1 fimbriae and curli (P < 0.05). Unlike the previously described aEPEC O55:H7, one aEPEC O119:HND strain (A111) formed a strong biofilm and pellicle at the air-liquid interface, but did not express curli. Transposon mutagenesis was used to identify biofilm-deficient mutants. Transposon insertion sequences of six mutants revealed similarity with type 1 fimbriae (fimC, fimD, and fimH), diguanylate cyclase, ATP synthase F1, beta subunit (atpD), and the uncharacterized YjiC protein. All these mutants were deficient in biofilm formation ability.Conclusion: This study showed that the ability to adhere to abiotic surfaces and form biofilm is present in an array of aEPEC strains. Moreover, it seems that the ability to form biofilms is associated with the presence of type 1 fimbriae and diguanylate cyclase. Characterization of additional biofilm formation mutants may reveal other mechanisms involved in biofilm formation and bring new insights into aEPEC adhesion and pathogenesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, BR-04023062 São Paulo, BrazilWeb of Science7engBiomed Central LtdBmc MicrobiologyEnteropathogenic Escherichia coliBiofilm formationType 1 piliPhenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000339080400001.pdfapplication/pdf714030${dspace.ui.url}/bitstream/11600/37987/1/WOS000339080400001.pdfd0a37a46a45e7261220bfedde6afcae5MD51open accessTEXTWOS000339080400001.pdf.txtWOS000339080400001.pdf.txtExtracted texttext/plain31869${dspace.ui.url}/bitstream/11600/37987/2/WOS000339080400001.pdf.txte13ab4a828cccc46a35466e80642a8b8MD52open access11600/379872022-11-04 15:31:41.756open accessoai:repositorio.unifesp.br:11600/37987Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:29:25.637170Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
title |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
spellingShingle |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains Nascimento, Heloisa H. [UNIFESP] Enteropathogenic Escherichia coli Biofilm formation Type 1 pili |
title_short |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
title_full |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
title_fullStr |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
title_full_unstemmed |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
title_sort |
Phenotypic and genotypic characteristics associated with biofilm formation in clinical isolates of atypical enteropathogenic Escherichia coli (aEPEC) strains |
author |
Nascimento, Heloisa H. [UNIFESP] |
author_facet |
Nascimento, Heloisa H. [UNIFESP] Silva, Lucas E. P. [UNIFESP] Souza, Renata T. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author_role |
author |
author2 |
Silva, Lucas E. P. [UNIFESP] Souza, Renata T. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Nascimento, Heloisa H. [UNIFESP] Silva, Lucas E. P. [UNIFESP] Souza, Renata T. [UNIFESP] Silva, Neusa Pereira da [UNIFESP] Scaletsky, Isabel Cristina Affonso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Enteropathogenic Escherichia coli Biofilm formation Type 1 pili |
topic |
Enteropathogenic Escherichia coli Biofilm formation Type 1 pili |
description |
Background: Biofilm formation by enteropathogenic Escherichia coli (EPEC) have been recently described in the prototype typical EPEC E2348/69 strain and in an atypical EPEC O55:H7 strain. in this study, we sought to evaluate biofilm formation in a collection of 126 atypical EPEC strains isolated from 92 diarrheic and 34 nondiarrheic children, belonging to different serotypes. the association of biofilm formation and adhesin-related genes were also investigated.Results: Biofilm formation occurred in 37 (29%)strains of different serotypes, when the assays were performed at 26 degrees C and 37 degrees C for 24 h. Among these, four strains (A79, A87, A88, and A111) formed a stronger biofilm than did the others. the frequency of biofilm producers was higher among isolates from patients compared with isolates from controls (34.8% vs 14.7%; P = 0.029). An association was found between biofilm formation and expression of type 1 fimbriae and curli (P < 0.05). Unlike the previously described aEPEC O55:H7, one aEPEC O119:HND strain (A111) formed a strong biofilm and pellicle at the air-liquid interface, but did not express curli. Transposon mutagenesis was used to identify biofilm-deficient mutants. Transposon insertion sequences of six mutants revealed similarity with type 1 fimbriae (fimC, fimD, and fimH), diguanylate cyclase, ATP synthase F1, beta subunit (atpD), and the uncharacterized YjiC protein. All these mutants were deficient in biofilm formation ability.Conclusion: This study showed that the ability to adhere to abiotic surfaces and form biofilm is present in an array of aEPEC strains. Moreover, it seems that the ability to form biofilms is associated with the presence of type 1 fimbriae and diguanylate cyclase. Characterization of additional biofilm formation mutants may reveal other mechanisms involved in biofilm formation and bring new insights into aEPEC adhesion and pathogenesis. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-10 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:35Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 7 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37987 http://dx.doi.org/10.1186/1471-2180-14-184 |
dc.identifier.issn.none.fl_str_mv |
1471-2180 |
dc.identifier.file.none.fl_str_mv |
WOS000339080400001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2180-14-184 |
dc.identifier.wos.none.fl_str_mv |
WOS:000339080400001 |
identifier_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, 7 p., 2014. 1471-2180 WOS000339080400001.pdf 10.1186/1471-2180-14-184 WOS:000339080400001 |
url |
http://repositorio.unifesp.br/handle/11600/37987 http://dx.doi.org/10.1186/1471-2180-14-184 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Bmc Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/37987/1/WOS000339080400001.pdf ${dspace.ui.url}/bitstream/11600/37987/2/WOS000339080400001.pdf.txt |
bitstream.checksum.fl_str_mv |
d0a37a46a45e7261220bfedde6afcae5 e13ab4a828cccc46a35466e80642a8b8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460297982345216 |