Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26535 http://dx.doi.org/10.1038/sj.bjp.0704069 |
Resumo: | 1 the present study analyses the short- (15 min-2 h) and long-term (24-48 h) influences of calcitonin gene-related peptide (CGRP) on acetylcholinesterase (AChE) expression in the rat cultured skeletal muscle and the signal transduction events underlying CGRP actions.2 To assess the effect of CGRP on AChE synthesis, myotubes were pre-exposed to the irreversible AChE inhibitor diisopropyl fluorophosphate (DFP) and treated with CGRP or forskolin, an adenylyl cyclase (AC) activator. Treatment of myotubes with 1 - 100 nM CGRP for 2 h increased by up to 42% the synthesis of catalytically active AChE with a parallel increase in the intracellular cyclic AMP.3 the stimulation of AChE synthesis induced by CGRP was mimicked by direct activation of AC with 3 - 30 muM forskolin. in contrast, pre-treatment of cultures with 100 nM CGRP for 20 h reduced by 37% the subsequent synthesis of AChE, resulting in a 15% decrease in total AChE activity after 48 h CGRP treatment.4 Moreover, 24 h treatment of myotubes with 100 nM CGRP reduced by 54% the accumulation of cyclic AMP induced by a subsequent CGRP treatment.5 These findings indicate that, in skeletal muscle cells, CGRP modulates the AChE expression in a time-dependent manner, initially stimulating the enzyme synthesis through a cyclic AMP-dependent mechanism. the decreased AChE synthesis observed after long-term CGRP treatment suggests that CGRP signalling system is subject to desensitization or down-regulation, that might function as an important adaptative mechanism of the muscle fibre in response to long-term changes in neuromuscular transmission. |
id |
UFSP_a204f826069b1d744515d92a75686555 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/26535 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Costa Junior, Valter Luiz daLapa, Antonio José [UNIFESP]Godinho, Rosely Oliveira [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:31:22Z2016-01-24T12:31:22Z2001-05-01British Journal of Pharmacology. Basingstoke: Nature Publishing Group, v. 133, n. 2, p. 229-236, 2001.0007-1188http://repositorio.unifesp.br/handle/11600/26535http://dx.doi.org/10.1038/sj.bjp.070406910.1038/sj.bjp.0704069WOS:0001687508000021 the present study analyses the short- (15 min-2 h) and long-term (24-48 h) influences of calcitonin gene-related peptide (CGRP) on acetylcholinesterase (AChE) expression in the rat cultured skeletal muscle and the signal transduction events underlying CGRP actions.2 To assess the effect of CGRP on AChE synthesis, myotubes were pre-exposed to the irreversible AChE inhibitor diisopropyl fluorophosphate (DFP) and treated with CGRP or forskolin, an adenylyl cyclase (AC) activator. Treatment of myotubes with 1 - 100 nM CGRP for 2 h increased by up to 42% the synthesis of catalytically active AChE with a parallel increase in the intracellular cyclic AMP.3 the stimulation of AChE synthesis induced by CGRP was mimicked by direct activation of AC with 3 - 30 muM forskolin. in contrast, pre-treatment of cultures with 100 nM CGRP for 20 h reduced by 37% the subsequent synthesis of AChE, resulting in a 15% decrease in total AChE activity after 48 h CGRP treatment.4 Moreover, 24 h treatment of myotubes with 100 nM CGRP reduced by 54% the accumulation of cyclic AMP induced by a subsequent CGRP treatment.5 These findings indicate that, in skeletal muscle cells, CGRP modulates the AChE expression in a time-dependent manner, initially stimulating the enzyme synthesis through a cyclic AMP-dependent mechanism. the decreased AChE synthesis observed after long-term CGRP treatment suggests that CGRP signalling system is subject to desensitization or down-regulation, that might function as an important adaptative mechanism of the muscle fibre in response to long-term changes in neuromuscular transmission.Universidade Federal de São Paulo, Dept Pharmacol, Escola Paulista Med, INFAR, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, Escola Paulista Med, INFAR, BR-04044020 São Paulo, BrazilWeb of Science229-236engNature Publishing GroupBritish Journal of Pharmacologycalcitonin gene-related peptideacetylcholinesteraseadenylyl cyclaseneuromuscular junctionskeletal muscleShort- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/265352022-09-27 09:38:14.905metadata only accessoai:repositorio.unifesp.br:11600/26535Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T12:38:14Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
title |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
spellingShingle |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes Costa Junior, Valter Luiz da calcitonin gene-related peptide acetylcholinesterase adenylyl cyclase neuromuscular junction skeletal muscle |
title_short |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
title_full |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
title_fullStr |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
title_full_unstemmed |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
title_sort |
Short- and long-term influences of calcitonin gene-related peptide on the synthesis of acetylcholinesterase in mammalian myotubes |
author |
Costa Junior, Valter Luiz da |
author_facet |
Costa Junior, Valter Luiz da Lapa, Antonio José [UNIFESP] Godinho, Rosely Oliveira [UNIFESP] |
author_role |
author |
author2 |
Lapa, Antonio José [UNIFESP] Godinho, Rosely Oliveira [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Costa Junior, Valter Luiz da Lapa, Antonio José [UNIFESP] Godinho, Rosely Oliveira [UNIFESP] |
dc.subject.eng.fl_str_mv |
calcitonin gene-related peptide acetylcholinesterase adenylyl cyclase neuromuscular junction skeletal muscle |
topic |
calcitonin gene-related peptide acetylcholinesterase adenylyl cyclase neuromuscular junction skeletal muscle |
description |
1 the present study analyses the short- (15 min-2 h) and long-term (24-48 h) influences of calcitonin gene-related peptide (CGRP) on acetylcholinesterase (AChE) expression in the rat cultured skeletal muscle and the signal transduction events underlying CGRP actions.2 To assess the effect of CGRP on AChE synthesis, myotubes were pre-exposed to the irreversible AChE inhibitor diisopropyl fluorophosphate (DFP) and treated with CGRP or forskolin, an adenylyl cyclase (AC) activator. Treatment of myotubes with 1 - 100 nM CGRP for 2 h increased by up to 42% the synthesis of catalytically active AChE with a parallel increase in the intracellular cyclic AMP.3 the stimulation of AChE synthesis induced by CGRP was mimicked by direct activation of AC with 3 - 30 muM forskolin. in contrast, pre-treatment of cultures with 100 nM CGRP for 20 h reduced by 37% the subsequent synthesis of AChE, resulting in a 15% decrease in total AChE activity after 48 h CGRP treatment.4 Moreover, 24 h treatment of myotubes with 100 nM CGRP reduced by 54% the accumulation of cyclic AMP induced by a subsequent CGRP treatment.5 These findings indicate that, in skeletal muscle cells, CGRP modulates the AChE expression in a time-dependent manner, initially stimulating the enzyme synthesis through a cyclic AMP-dependent mechanism. the decreased AChE synthesis observed after long-term CGRP treatment suggests that CGRP signalling system is subject to desensitization or down-regulation, that might function as an important adaptative mechanism of the muscle fibre in response to long-term changes in neuromuscular transmission. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:31:22Z |
dc.date.available.fl_str_mv |
2016-01-24T12:31:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
British Journal of Pharmacology. Basingstoke: Nature Publishing Group, v. 133, n. 2, p. 229-236, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26535 http://dx.doi.org/10.1038/sj.bjp.0704069 |
dc.identifier.issn.none.fl_str_mv |
0007-1188 |
dc.identifier.doi.none.fl_str_mv |
10.1038/sj.bjp.0704069 |
dc.identifier.wos.none.fl_str_mv |
WOS:000168750800002 |
identifier_str_mv |
British Journal of Pharmacology. Basingstoke: Nature Publishing Group, v. 133, n. 2, p. 229-236, 2001. 0007-1188 10.1038/sj.bjp.0704069 WOS:000168750800002 |
url |
http://repositorio.unifesp.br/handle/11600/26535 http://dx.doi.org/10.1038/sj.bjp.0704069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
British Journal of Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
229-236 |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764160155516928 |