Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55497 https://dx.doi.org/10.1590/S1678-9946201759045 |
Resumo: | Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix r (G1P[8]) was introduced into Brazil's National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (Sao Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0- 4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix (R). |
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59Leite, Marcel [UNIFESP]Carmona, Rita Cássia CompagnoliCarraro, EmersonWatanabe, Aripuanã Sakurada Aranha [UNIFESP]Granato, Celso Francisco Hernandes [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Sao Paulo2020-07-17T14:03:33Z2020-07-17T14:03:33Z2017Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix r (G1P[8]) was introduced into Brazil's National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (Sao Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0- 4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix (R).Grp Fleury Automacao, Av Gen Waldomiro Lima 508, BR-04344070 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Med, Lab Virol Clin, Unidade Doencas Infecciosas, Sao Paulo, SP, BrazilAdolfo Lutz Inst, Nucl Doencas Enter, Ctr Virol, Sao Paulo, SP, BrazilUniv Estadual Centro Oeste, Guarapuava, Parana, BrazilUniv Fed Sao Paulo, Dept Med, Lab Virol Clin, Unidade Doencas Infecciosas, Sao Paulo, SP, BrazilWeb of ScienceFederal University of Sao PauloFleury Group-Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017.10.1590/S1678-9946201759045S0036-46652017005000223.pdf0036-4665S0036-46652017005000223https://repositorio.unifesp.br/handle/11600/55497https://dx.doi.org/10.1590/S1678-9946201759045WOS:000407427600002engInst Medicina Tropical Sao PauloRevista Do Instituto De Medicina Tropical De Sao PauloRotavirusEpidemiologyGenotypeVaccineRotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0036-46652017005000223.pdfapplication/pdf392329https://repositorio.unifesp.br/bitstreams/41a8a9ae-067a-4b71-95c0-c48a67488ed6/downloadbc64bfee5467ef4331675d1baf1f5707MD5111600/554972024-01-25 19:50:29.923oai:repositorio.unifesp.br/:11600/55497https://repositorio.unifesp.brRepositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652024-01-25T19:50:29Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
title |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
spellingShingle |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil Leite, Marcel [UNIFESP] Rotavirus Epidemiology Genotype Vaccine |
title_short |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
title_full |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
title_fullStr |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
title_full_unstemmed |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
title_sort |
Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil |
author |
Leite, Marcel [UNIFESP] |
author_facet |
Leite, Marcel [UNIFESP] Carmona, Rita Cássia Compagnoli Carraro, Emerson Watanabe, Aripuanã Sakurada Aranha [UNIFESP] Granato, Celso Francisco Hernandes [UNIFESP] |
author_role |
author |
author2 |
Carmona, Rita Cássia Compagnoli Carraro, Emerson Watanabe, Aripuanã Sakurada Aranha [UNIFESP] Granato, Celso Francisco Hernandes [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Leite, Marcel [UNIFESP] Carmona, Rita Cássia Compagnoli Carraro, Emerson Watanabe, Aripuanã Sakurada Aranha [UNIFESP] Granato, Celso Francisco Hernandes [UNIFESP] |
dc.subject.eng.fl_str_mv |
Rotavirus Epidemiology Genotype Vaccine |
topic |
Rotavirus Epidemiology Genotype Vaccine |
description |
Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix r (G1P[8]) was introduced into Brazil's National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (Sao Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0- 4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix (R). |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-17T14:03:33Z |
dc.date.available.fl_str_mv |
2020-07-17T14:03:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/55497 https://dx.doi.org/10.1590/S1678-9946201759045 |
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10.1590/S1678-9946201759045 |
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S0036-46652017005000223.pdf |
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0036-4665 |
dc.identifier.scielo.none.fl_str_mv |
S0036-46652017005000223 |
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WOS:000407427600002 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017. 10.1590/S1678-9946201759045 S0036-46652017005000223.pdf 0036-4665 S0036-46652017005000223 WOS:000407427600002 |
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https://repositorio.unifesp.br/handle/11600/55497 https://dx.doi.org/10.1590/S1678-9946201759045 |
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Revista Do Instituto De Medicina Tropical De Sao Paulo |
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Sao Paulo |
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Inst Medicina Tropical Sao Paulo |
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Inst Medicina Tropical Sao Paulo |
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