Concentração de proteínas no humor aquoso de pacientes com glaucoma primário de ângulo aberto em tratamento clínico

• Autor(a) principal: Prata, Tiago dos Santos [UNIFESP]
• Data de Publicação: 2007
• Outros Autores: Navajas, Eduardo Vitor [UNIFESP], Melo Junior, Luiz Alberto Soares [UNIFESP], Martins, João Roberto Maciel [UNIFESP], Nader, Helena Bonciani [UNIFESP], Belfort, Rubens Junior [UNIFESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0004-27492007000200006; http://repositorio.unifesp.br/handle/11600/3608
• topic: Glaucoma, open-angle; Proteins; Aqueous humor; Cataract; Intraocular pressure; Comparative study; Glaucoma de ângulo aberto; Proteínas; Humor aquoso; Catarata; Pressão intra-ocular; Estudo comparativo

PURPOSE: To compare total protein concentration in the aqueous humor of primary open-angle glaucoma and non-glaucomatous patients. METHODS: Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled primary open angle glaucoma (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed in 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20°C. Aqueous humor total protein concentration was determined using a colorimetric assay. RESULTS: The geometric mean of total protein concentration of the aqueous humor samples was 32 mg/dL (range: 8-137 mg/dL) in the primary open angle glaucoma group and 16 mg/dL (range: 2-85 mg/dL) in the control group. The ratio of the protein concentration between the two groups was 2.0 (95% confidence interval: 1.3 to 3.2; p=0.003). CONCLUSIONS: The total protein concentration in primary open-angle glaucoma aqueous humor was approximately two times higher than that in non-glaucomatous subjects.