Immunologic features of HIV-1-infected women on HAART at delivery

• Autor(a) principal: Ono, Erika [UNIFESP]
• Data de Publicação: 2008
• Outros Autores: Dos Santos, Amelia Miyashiro [UNIFESP], Machado, Daisy Maria [UNIFESP], Succi, Regina Célia de Menezes [UNIFESP], Amed, Abes Mahmed [UNIFESP], Salomão, Reinaldo [UNIFESP], Kallas, Esper Georges [UNIFESP], De Moraes-Pinto, Maria Isabel [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/0013000019959
• Texto Completo: http://dx.doi.org/10.1002/cyto.b.20418; http://repositorio.unifesp.br/handle/11600/30739
• topic: immunophenotyping; HAART; gestation; HIV

Background: the conjoint effect of HIV infection and pregnancy on the immune system of women submitted to the prophylactic antiretroviral therapy presently recommended is still poorly understood.Methods: We evaluated 44 HIV-infected women (HIV) and 45 HIV-negative women (CT) at parturition and we compared them to 20 healthy nonpregnant women (NP). Immunophenotyping of lymphocytes was done by four-color flow cytometry.Results: All HIV-infected women received HAART during pregnancy and 56.8% had viral load <50 copies/mL at delivery. CD4+T cells/mm(3) were lower in HIV (447) than CT (593) and NP (738) (P < 0.05). CD8+T cells/mm(3) were higher in HIV (799) than CT (384) and NP (395) (P < 0.05). NK cells/mm(3) were lower in HIV (146) than in CT (253) and NP (198) (P < 0.05). CD38 expression on CD4+T and on CD8+T cells was higher in HIV (CD4:12.1; CD8:14.9) than in CT(CD4:9.2; CD8:10.2) and NP(CD4:8.6; CD8:6.0) (P < 0.05). However, CD56 expression on CD8+T cells (a marker of cytolytic effector function) was lower in HIV(7%) than in CT(12%) and NP(9%) (P < 0.05).Conclusions: Even with low levels of viremia, HIV-infected women at delivery showed a different immunologic profile from both healthy non-HIV-infected women in the puerperium and nonpregnant women, with lower CD4+T and higher CD8+T cells, high levels of CD38 expression, but low CD56 expression on CD8+T cells and low NK cell numbers. (C) 2008 Clinical Cytometry Society.